Overexpression of microRNA gga-miR-21 in chicken fibroblasts suppresses replication of infectious bursal disease virus through inhibiting VP1 translation

Wang, Yongshan; Ouyang, Wei; Pan, Qunxing; Wang, Xiaoli; Xia, Xingxia; Bi, Zhenwei; Wang, Yongqiang; Wang, Xiaomei

doi:10.1016/j.antiviral.2013.08.001

Cited by 43 publications

(29 citation statements)

References 23 publications

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“…Recently, miRNAs, as new players of regulatory control over gene expression programs, have been well studied, especially in human cancers. The role of miRNAs in host defense against viral infections has also been well illustrated (12,54,55). It was reported that host miRNAs could inhibit virus replication via direct targeting of the viral genome in the process of viral infection, suggesting a new kind of host antiviral mechanism (11,39,56).…”

Section: Discussionmentioning

confidence: 95%

MicroRNA gga-miR-130b Suppresses Infectious Bursal Disease Virus Replication via Targeting of the Viral Genome and Cellular Suppressors of Cytokine Signaling 5

Wang

Chen

et al. 2018

J Virol

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MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression posttranscriptionally through silencing or degrading their targets, thus playing important roles in the immune response. However, the role of miRNAs in the host response against infectious bursal disease virus (IBDV) infection is not clear. In this study, we show that the expression of a series of miRNAs was significantly altered in DF-1 cells after IBDV infection. We found that the miRNA gga-miR-130b inhibited IBDV replication via targeting the specific sequence of IBDV segment A and enhanced the expression of beta interferon (IFN-β) by targeting suppressors of cytokine signaling 5 (SOCS5) in host cells. These findings indicate that gga-miR-130b-3p plays a crucial role in host defense against IBDV infection. This work shows that gga-miR-130b suppresses IBDV replication via directly targeting the viral genome and cellular SOCS5, the negative regulator for type I interferon expression, revealing the mechanism underlying gga-miR-130-induced inhibition of IBDV replication. This information will be helpful for the understanding of how host cells combat pathogenic infection by self-encoded small RNA and furthers our knowledge of the role of microRNAs in the cell response to viral infection.

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Section: Discussionmentioning

confidence: 95%

MicroRNA gga-miR-130b Suppresses Infectious Bursal Disease Virus Replication via Targeting of the Viral Genome and Cellular Suppressors of Cytokine Signaling 5

Wang

Chen

et al. 2018

J Virol

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“…Of these miRNAs, gga-let-7 g, gga-miR-196-2, gga-miR-1635, gga-miR-1603 and gga-miR-21 were significantly upregulated in IBDV-infected DCs. Previously, gga-miR-21 was suggested to inhibit the replication of IBDV by targeting and suppressing VP1 mRNA translation [30]. This suggests that, in chickens, gga-miR-21 is upregulated as a defence mechanism to fight against IBDV by inhibiting viral replication.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide profiling of chicken dendritic cell response to infectious bursal disease

et al. 2016

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BackgroundAvian infectious bursal disease virus (IBDV) is a highly contagious, immunosuppressive disease of young chickens, which causes high mortality rates and large economic losses in the poultry industry. Dendritic cells (DCs), which are antigen-presenting cells, have the unique ability to induce both innate and acquired immune responses and may significantly influence virus pathogenicity. To understand the interaction between IBDV and DCs, a microarray was used to analyse the response of DCs infected by IBDV.ResultsIBDV infection induced 479 upregulated and 466 downregulated mRNAs in chicken DCs. Analysis of Gene Ontology suggested that transcription from the RNA polymerase II promoter and the RNA biosynthetic process were enriched, and pathway analyses suggested that oxidative phosphorylation, as well as the T cell receptor and Interleukin-17 (IL-17) signalling pathways might be activated by IBDV infection. Moreover, microRNA (miRNA) and long non-coding RNA (lncRNA) alterations in IBDV-infected chicken DCs were observed. A total of 18 significantly upregulated or downregulated miRNAs and 441 significantly upregulated or downregulated lncRNAs were identified in IBDV-stimulated DCs. We constructed 42 transcription factor (TF)–miRNA–mRNA interactions involving 1 TF, 3 miRNAs, and 42 mRNAs in IBDV-stimulated DCs. Finally, we predicted the target genes of differentially expressed lncRNAs, and constructed lncRNA-mRNA regulatory networks.ConclusionsThe results of this study suggest a mechanism to explain how IBDV infection triggers an effective immune response in chicken DCs.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3157-5) contains supplementary material, which is available to authorized users.

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“…Similarly, gga-miR-454 was found to inhibit IBDV replication by binding to a specific sequence of IBDV segment B and enhancing the expression of IFN-β by targeting cellular SOCS6 (Table 1, Fu et al, 2018). Additionally, gga-miR-21 could target viral genomic RNA and inhibit VP1 translation to lower IBDV Journal of Poultry Science, 57 (1) (Table 1, Wang et al, 2013a).…”

Section: Mirna and Infectious Bursal Diseasementioning

confidence: 99%

Research Progress on MicroRNAs Involved in the Regulation of Chicken Diseases

Wang

2020

J. Poult. Sci.

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MicroRNAs (miRNAs) are small, non-coding RNA molecules that inhibit protein translation from target mRNAs. Accumulating evidence suggests that miRNAs can regulate a broad range of biological pathways, including cell differentiation, apoptosis, and carcinogenesis. With the development of miRNAs, the investigation of miRNA functions has emerged as a hot research field. Due to the intensive farming in recent decades, chickens are easily influenced by various pathogen transmissions, and this has resulted in large economic losses. Recent reports have shown that miRNAs can play critical roles in the regulation of chicken diseases. Therefore, the aim of this review is to briefly discuss the current knowledge regarding the effects of miRNAs on chickens suffering from common viral diseases, mycoplasmosis, necrotic enteritis, and ovarian tumors. Additionally, the detailed targets of miRNAs and their possible functions are also summarized. This review intends to highlight the key role of miRNAs in regard to chickens and presents the possibility of improving chicken disease resistance through the regulation of miRNAs.

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Overexpression of microRNA gga-miR-21 in chicken fibroblasts suppresses replication of infectious bursal disease virus through inhibiting VP1 translation

Cited by 43 publications

References 23 publications

MicroRNA gga-miR-130b Suppresses Infectious Bursal Disease Virus Replication via Targeting of the Viral Genome and Cellular Suppressors of Cytokine Signaling 5

MicroRNA gga-miR-130b Suppresses Infectious Bursal Disease Virus Replication via Targeting of the Viral Genome and Cellular Suppressors of Cytokine Signaling 5

Genome-wide profiling of chicken dendritic cell response to infectious bursal disease

Research Progress on MicroRNAs Involved in the Regulation of Chicken Diseases

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