Overexpression of miR-99a Alleviates Intestinal Mucosal Barrier Injury in Rats with Severe Acute Pancreatitis

Shao, Feifei; Guan, Zhihui; Luo, Jing; Xiao, Xiaorong; Zhou, Lingmin

doi:10.1089/jir.2020.0085

Cited by 5 publications

(4 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 2019 ; Zhang et al. 2021 ). Damage to intestinal epithelial cells, host immune deficiency and disturbance of intestinal flora can lead to increased intestinal permeability, which are the three key factors leading to intestinal barrier dysfunction and bacterial and endotoxin translocation (Pan et al.…”

Section: Discussionmentioning

confidence: 99%

Da-Cheng-Qi decoction improves severe acute pancreatitis-associated acute lung injury by interfering with intestinal lymphatic pathway and reducing HMGB1-induced inflammatory response in rats

Liu

Yuan

Tang

et al. 2023

Pharmaceutical Biology

View full text Add to dashboard Cite

Context Da-Cheng-Qi Decoction (DCQD) has a significant effect on Severe Acute Pancreatitis-Associated Acute Lung Injury (SAP-ALI). Objective To explore the mechanism of DCQD in the treatment of SAP-ALI based on intestinal barrier function and intestinal lymphatic pathway. Materials and methods Forty-five Sprague-Dawley rats were divided into three groups: sham operation, model, and DCQD. The SAP model was induced by a retrograde infusion of 5.0% sodium taurocholate solution (1 mg/kg) at a constant rate of 12 mL/h using an infusion pump into the bile-pancreatic duct. Sham operation and model group were given 0.9% normal saline, while DCQD group was given DCQD (5.99 g/kg/d) by gavage 1 h before operation and 1, 11 and 23 h after operation. The levels of HMGB1, RAGE, TNF-α, IL-6, ICAM-1, d -LA, DAO in blood and MPO in lung were detected using ELISA. The expression of HMGB1, RAGE, NF-κB p65 in mesenteric lymph nodes and lung were determined. Results Compared with SAP group, DCQD significantly reduced the histopathological scoring of pancreatic tissue (SAP, 2.80 ± 0.42; DCQD, 2.58 ± 0.52), intestine (SAP, 3.30 ± 0.68; DCQD, 2.50 ± 0.80) and lung (SAP, 3.30 ± 0.68; DCQD, 2.42 ± 0.52). DCQD reduced serum HMGB1 level (SAP, 134.09 ± 19.79; DCQD, 88.05 ± 9.19), RAGE level (SAP, 5.05 ± 1.44; DCQD, 2.13 ± 0.54). WB and RT-PCR showed HMGB1-RAGE pathway was inhibited by DCQD ( p < 0.01). Discussion and conclusions DCQD improves SAP-ALI in rats by interfering with intestinal lymphatic pathway and reducing HMGB1-induced inflammatory response.

show abstract

Section: Discussionmentioning

confidence: 99%

Da-Cheng-Qi decoction improves severe acute pancreatitis-associated acute lung injury by interfering with intestinal lymphatic pathway and reducing HMGB1-induced inflammatory response in rats

Liu

Yuan

Tang

et al. 2023

Pharmaceutical Biology

View full text Add to dashboard Cite

show abstract

“…MiR-99a overexpression in sodium taurocholate (NaT)-induced SAP rat model reduced pathologic injury in pancreatic and intestinal tissues by reducing circulating inflammatory cytokines IL-1β and TNFα and inflammation markers procalcitonin and endotoxin. ZO-1, occludin, and claudin expression were positively correlated with miR-99a levels [ 89 ] ( Figure 3 ). Similarly, miR-9 overexpressing bone marrow-derived mesenchymal stem cells (BMSCs) ameliorated pancreatic injury in SAP rats by inducing angiogenesis via increased Ang-1, TIE-2, CD31 expression, and activation of the PI3K/AKT pathway and inhibition of the VE-cadherin/β-catenin pathway ( Figure 3 ).…”

Section: Severe Acute Pancreatitismentioning

confidence: 99%

The Role of MicroRNAs in Pancreatitis Development and Progression

Patel

Almanzar

LaComb

et al. 2023

IJMS

View full text Add to dashboard Cite

Pancreatitis (acute and chronic) is an inflammatory disease associated with significant morbidity, including a high rate of hospitalization and mortality. MicroRNAs (miRs) are essential post-transcriptional modulators of gene expression. They are crucial in many diseases’ development and progression. Recent studies have demonstrated aberrant miRs expression patterns in pancreatic tissues obtained from patients experiencing acute and chronic pancreatitis compared to tissues from unaffected individuals. Increasing evidence showed that miRs regulate multiple aspects of pancreatic acinar biology, such as autophagy, mitophagy, and migration, impact local and systemic inflammation and, thus, are involved in the disease development and progression. Notably, multiple miRs act on pancreatic acinar cells and regulate the transduction of signals between pancreatic acinar cells, pancreatic stellate cells, and immune cells, and provide a complex interaction network between these cells. Importantly, recent studies from various animal models and patients’ data combined with advanced detection techniques support their importance in diagnosing and treating pancreatitis. In this review, we plan to provide an up-to-date summary of the role of miRs in the development and progression of pancreatitis.

show abstract

“…Due to their stability and ease of detection, miRs have the potential as biomarkers for AP diagnosis and assessment 8 . Unsurprisingly, a plethora of miRNAs are known to participate in the process of IBD in SAP 9–11 . Recent studies have further indicated that miR‐1 can protect against endothelial permeability and alleviate colitis symptoms by restoring impaired barrier function in rat colitis 12,13 .…”

Section: Introductionmentioning

confidence: 99%

“…8 Unsurprisingly, a plethora of miRNAs are known to participate in the process of IBD in SAP. [9][10][11] Recent studies have further indicated that miR-1 can protect against endothelial permeability and alleviate colitis symptoms by restoring impaired barrier function in rat colitis. 12,13 However, the role of serum miR-1-3p in SAP patients with IBD and its potential as an early predictor remains unclear.…”

mentioning

confidence: 99%

microRNA‐1‐3p and T‐synthase mRNA have high diagnostic efficacy on intestinal mucosal barrier dysfunction in patients with severe acute pancreatitis

Jiang

Chen

2023

The Kaohsiung J of Med Scie

View full text Add to dashboard Cite

Acute pancreatitis (AP) is an inflammatory disorder of the pancreas that can be complicated by intestinal mucosal barrier dysfunction (SAP&IBD). The current study sought to examine the diagnostic efficacy of miR‐1‐3p and T‐synthase mRNA in SAP&IBD patients. First, SAP patients were assigned to SAP&IBD and SAP groups. Serum miR‐1‐3p expression and T‐synthase mRNA expression patterns in peripheral blood B lymphocytes were measured using RT‐qPCR. Pearson tests, ROC curve analysis, and multivariate logistic regression were used to analyze the correlation between miR‐1‐3p/T‐synthase mRNA and clinical data, their diagnostic efficiency, and independent risk factors for SAP&IBD patients, respectively. The results showed that serum miR‐1‐3p in the SAP&IBD group was elevated, and T‐synthase mRNA expression in peripheral blood B lymphocytes was diminished. Additionally, serum miR‐1‐3p expression in SAP&IBD patients was negatively correlated with T‐synthase mRNA expression, and positively correlated with their Ranson score, CRP, IL‐6, DAO, and D‐Lactate levels. Meanwhile, T‐synthase mRNA level was negatively correlated with IL‐6, DAO, and D‐Lactate levels. Both, serum miR‐1‐3p, T‐synthase mRNA, and their combination were found to exhibit diagnostic efficiency for SAP&IBD patients, and were independently associated with IBD in SAP patients. Collectively, our findings suggest that miR‐1‐3p and T‐synthase serve as independent risk factors for SAP&IBD patients and can aid the diagnosis of IBD in SAP patients.

show abstract

Overexpression of miR-99a Alleviates Intestinal Mucosal Barrier Injury in Rats with Severe Acute Pancreatitis

Cited by 5 publications

References 43 publications

Da-Cheng-Qi decoction improves severe acute pancreatitis-associated acute lung injury by interfering with intestinal lymphatic pathway and reducing HMGB1-induced inflammatory response in rats

Da-Cheng-Qi decoction improves severe acute pancreatitis-associated acute lung injury by interfering with intestinal lymphatic pathway and reducing HMGB1-induced inflammatory response in rats

The Role of MicroRNAs in Pancreatitis Development and Progression

microRNA‐1‐3p and T‐synthase mRNA have high diagnostic efficacy on intestinal mucosal barrier dysfunction in patients with severe acute pancreatitis

Contact Info

Product

Resources

About