Overexpression of multiple cytochrome P450 genes associated with sulfoxaflor resistance in Aphis gossypii Glover

Ma, Kangsheng; Tang, Qiuling; Zhang, Baizhong; Liang, Pei; Wang, Baomin; Gao, Xiwu

doi:10.1016/j.pestbp.2019.03.021

Cited by 81 publications

(60 citation statements)

References 45 publications

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“…The overexpression of the P450 monooxygenase enzyme is the most common mechanism of imidacloprid and chlorpyrifos resistance [23][24][25]. In aphids, P450 monooxygenase enzyme also plays an important role in insecticide detoxification and resistance [26][27][28]. In the present study, samples treated with chlorpyrifos showed five upregulated P450 unigenes, as well as a fold change in imidacloprid-treated samples.…”

Section: Discussionsupporting

confidence: 53%

Transcriptome Analysis and Identification of Insecticide Tolerance-Related Genes after Exposure to Insecticide in Sitobion avenae

Wei

Zhong

Lin

et al. 2019

Genes

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Aphids cause serious losses to the production of wheat. The grain aphid, Sitobion avenae, which is the dominant species of aphid in all wheat regions of China, is resistant to a variety of insecticides, including imidacloprid and chlorpyrifos. However, the resistance and mechanism of insecticide tolerance of S. avenae are still unclear. Therefore, this study employed transcriptome analysis to compare the expression patterns of stress response genes under imidacloprid and chlorpyrifos treatment for 15 min, 3 h, and 36 h of exposure. S. avenae adult transcriptome was assembled and characterized first, after which samples treated with insecticides for different lengths of time were compared with control samples, which revealed 60–2267 differentially expressed unigenes (DEUs). Among these DEUs, 31–790 unigenes were classified into 66–786 categories of gene ontology (GO) functional groups, and 24–760 DEUs could be mapped into 54–268 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Finally, 11 insecticide-tolerance-related unigenes were chosen to confirm the relative expression by quantitative real-time polymerase chain reaction (qRT-PCR) in each treatment. Most of the results between qRT-PCR and RNA sequencing (RNA-Seq) are well-established. The results presented herein will facilitate molecular research investigating insecticide resistance in S. avenae, as well as in other wheat aphids.

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Section: Discussionsupporting

confidence: 53%

Transcriptome Analysis and Identification of Insecticide Tolerance-Related Genes after Exposure to Insecticide in Sitobion avenae

Wei

Zhong

Lin

et al. 2019

Genes

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“…The dsCHS1 (dsRNA of CHS1), dsGFP (dsRNA of GFP), or DEPC water was mixed in the synthetic feeding diet (0.5 mol/L sterile sucrose solution) at an ultimate concentration of 100 ng/µL and fed to the third-instar nymphs. For the in vitro feeding assays, sterilized glass tubes (3 cm in length and 2 cm in diameter) that open at both ends were used [43,44]. One end of each glass tube was wrapped with two coatings of parafilm membrane that contained a mixture of synthetic diet sandwiched between the two layers of parafilm.…”

Section: Dietary Delivery Of the Double-stranded Rna (Dsrna)mentioning

confidence: 99%

RNAi-Mediated Knockdown of Chitin Synthase 1 (CHS1) Gene Causes Mortality and Decreased Longevity and Fecundity in Aphis gossypii

Ullah

Gül

Wang

et al. 2019

Insects

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Chitin is a vital part of the insect exoskeleton and peritrophic membrane, synthesized by chitin synthase (CHS) enzymes. Chitin synthase 1 (CHS1) is a crucial enzyme in the final step of chitin biosynthetic pathway and consequently plays essential role towards insect growth and molting. RNA interference (RNAi) is an agent that could be used as an extremely target-specific and ecologically innocuous tactic to control different insect pests associated with economically important crops. The sole purpose of the current study is to use CHS1 as the key target gene against the cotton-melon aphid, Aphis gossypii, via oral feeding on artificial diets mixed with dsRNA-CHS1. Results revealed that the expression level of CHS1 gene significantly decreased after the oral delivery of dsRNA-CHS1. The knockdown of CHS1 gene caused up to 43%, 47%, and 59% mortality in third-instar nymph after feeding of dsCHS1 for 24, 48, and 72 h, respectively, as compared to the control. Consistent with this, significantly lower longevity (approximately 38%) and fecundity (approximately 48%) were also found in adult stage of cotton-melon aphids that were fed with dsCHS1 for 72 h at nymphal stage. The qRT-PCR analysis of gene expression demonstrated that the increased mortality rates and lowered longevity and fecundity of A. gossypii were attributed to the downregulation of CHS1 gene via oral-delivery-mediated RNAi. The results of current study confirm that CHS1 could be an appropriate candidate target gene for the RNAi-based control of cotton-melon aphids.

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“…However, both PBO and DEF significantly increased the toxicity of sulfoxaflor in A . gossypii 108 . PBO did not involve increasing susceptibility in many insect pests to many different insecticides, such as chlorfenapyr in O .…”

Section: Discussionmentioning

confidence: 86%

Inheritance mode and metabolic mechanism of the sulfoximine insecticide sulfoxaflor resistance in Oxycarenus hyalinipennis (Costa)

Wazir

Shad

2021

Pest Management Science

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BACKGROUND Dusky cotton bug (DCB), Oxycarenus hyalinipennis (Costa) (Hemiptera: Lygaeidae), is a key insect pest of cotton. It causes huge losses to cotton and many other economically important crops. Sulfoxaflor is a newly introduced systemic insecticide that is effective against many sap‐feeding insect pests such as aphids, whiteflies and true bugs. The present study was designed to characterize the inheritance of sulfoxaflor resistance in DCB. Moreover, the role of synergists in reducing sulfoxaflor resistance in DCB was also assessed. RESULTS A field population of DCB has developed 1132.0‐fold resistance to sulfoxaflor after 11 selected generations in the laboratory. Nonsignificant difference of reciprocal crosses was observed depending on the LC50 (median lethal concentration) values (95% confidence intervals overlapped), suggesting an autosomal mode of sulfoxaflor resistance inheritance. The degree of dominance of 0.7 for F1 (Sulfo‐Sel Pop ♀ × Lab‐Pop♂) and 0.6 for F1'(Sulfo‐Sel Pop ♂ × Lab‐Pop♀), respectively, suggested that sulfoxaflor resistance was incompletely dominant. According to the monogenic model, the number of genes involved to induce sulfoxaflor resistance revealed that sulfoxaflor resistance was polygenic in nature. The realized heritability (h2) value for sulfoxaflor resistance was 0.2. The synergists experiment indicated that esterases were involved in the sulfoxaflor resistance mechanism in DCB. CONCLUSIONS The current results indicate that there is autosomal, incompletely dominant and polygenic inheritance of sulfoxaflor resistance in DCB. Our results would be helpful in delaying sulfoxaflor resistance against DCB in the field. © 2021 Society of Chemical Industry

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Overexpression of multiple cytochrome P450 genes associated with sulfoxaflor resistance in Aphis gossypii Glover

Cited by 81 publications

References 45 publications

Transcriptome Analysis and Identification of Insecticide Tolerance-Related Genes after Exposure to Insecticide in Sitobion avenae

Transcriptome Analysis and Identification of Insecticide Tolerance-Related Genes after Exposure to Insecticide in Sitobion avenae

RNAi-Mediated Knockdown of Chitin Synthase 1 (CHS1) Gene Causes Mortality and Decreased Longevity and Fecundity in Aphis gossypii

Inheritance mode and metabolic mechanism of the sulfoximine insecticide sulfoxaflor resistance in Oxycarenus hyalinipennis (Costa)

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