Overexpression of Murine Pax3 Increases NCAM Polysialylation in a Human Medulloblastoma Cell Line

Mayanil, C. Shekhar; George, David; Mania-Farnell, Barbara; Bremer, Christopher L.; McLone, David G.; Bremer, E.

doi:10.1074/jbc.m002975200

Cited by 45 publications

(43 citation statements)

References 37 publications

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“…We also determined that while CXCR4 is strongly induced by Pax3/FKHR in RD cells (data not shown), as was demonstrated previously (Libura et al, 2002), no change in expression was seen in Pax3/FKHR-expressing SaOS-2 cells. Thus, comparisons between NIH3T3 cells (Khan et al, 1999) or medulloblastomas (Mayanil et al, 2000(Mayanil et al, , 2001 with SaOS-2, RD or RH30 cells is likely to reveal divergent sets of gene targets. These data further imply significant limitations in extrapolating microarray data derived from individual cell lines to other rhabdomyosarcomas or to identification of target genes during, for example, Pax3-dependent neural crest cell migration, somite development or in rhabdomyosarcomas.…”

Section: Discussionmentioning

confidence: 99%

Cell-type-specific regulation of distinct sets of gene targets by Pax3 and Pax3/FKHR

et al. 2005

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Section: Discussionmentioning

confidence: 99%

Cell-type-specific regulation of distinct sets of gene targets by Pax3 and Pax3/FKHR

et al. 2005

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“…We showed by immuno¯uorescence, Western blot, and cytometric analyses, that all TE 671 cells strongly expressed PSA-NCAM. Interestingly, Pax-3, a developmentally regulated transcription factor, is thought to be involved in the regulation of polysialyltransferases (PSTs) responsible for the expression of PSA (Mayanil et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

A nude mice model of human rhabdomyosarcoma lung metastases for evaluating the role of polysialic acids in the metastatic process

Daniel

Durbec²,

Gautherot³

et al. 2001

Oncogene

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PSA is an oncodevelopmental antigen usually expressed in human tumors with high metastatic potential. Here we set up a metastatic model in nude mice by using TE671 cells, which strongly express PSA-NCAM. We observed the formation of lung metastases when TE671 cells were injected intravenously, intramuscularly, and intraperitoneously, but not subcutaneously. Intraperitoneous injections also induced peritoneal carcinosis, ascites, and liver metastases. To evaluate the putative role of PSA in the metastatic process we used a speci®c cleavage of PSA on NCAM by endoneuraminidase-N on intraperitoneous primary tumors. Mice with primary intramuscular tumors were taken as control. Repeated injections of endoneuraminidase-N led to a decrease in PSA expression in primary intraperitoneous nodules and ascites but not in intramuscular primary tumors. Endoneuraminidase-N also increased the delay in ascitic formation and decreased the number of lung or liver metastases in the case of intraperitoneous tumors but not in the case of intramuscular tumors. When metastases occurred in endoneuraminidase-N injected animals, they strongly expressed PSA-NCAM. Therefore, we established a relationship between PSA expression on the surface of primary tumor cells and the metastatic process. Oncogene (2001) 20, 997 ± 1004.

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“…Loss or loosening of cellular adhesion is often correlated with early stages of tumor progression as cells break away from the primary tumor. Overexpression of mouse Pax3 in a human medulloblastoma cell line had a direct effect on cell adhesion (32). Whether PAX3 isoforms influence melanocyte migration by altering adhesion needs clarification.…”

Section: Discussionmentioning

confidence: 99%

Functional Analysis of Alternative Isoforms of the Transcription Factor PAX3 in Melanocytes In vitro

et al. 2006

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Transcription factor PAX3 has seven isoforms of which PAX3c has been studied extensively whereas the functions of the other isoforms are less well known. Here, we found that PAX3 isoforms in a stable transfection system have different biological functions in mouse melanocytes in vitro. PAX3a and PAX3b had negative effects on melanocyte proliferation but had no discernable effect on melanocyte growth in soft agar. PAX3a did not affect cell migration and apoptosis but PAX3b reduced migration and accelerated apoptosis. PAX3c and PAX3d promoted cell proliferation, migration, transformation, and survival. PAX3e reduced melanocyte growth; transformation and migration were unchanged and apoptosis was increased in vitro. PAX3g did not influence cell proliferation or apoptosis. Cells expressing PAX3g were able to grow in soft agar but migration was reduced. PAX3h increased cell proliferation, migration, survival, and transformation. These functional studies have advanced our understanding of the effects of PAX3 isoforms in melanocytes and their potential contribution in tumorigenesis. (Cancer Res 2006; 66(17): 8574-80)

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Overexpression of Murine Pax3 Increases NCAM Polysialylation in a Human Medulloblastoma Cell Line

Cited by 45 publications

References 37 publications

Cell-type-specific regulation of distinct sets of gene targets by Pax3 and Pax3/FKHR

Cell-type-specific regulation of distinct sets of gene targets by Pax3 and Pax3/FKHR

A nude mice model of human rhabdomyosarcoma lung metastases for evaluating the role of polysialic acids in the metastatic process

Functional Analysis of Alternative Isoforms of the Transcription Factor PAX3 in Melanocytes In vitro

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