Overexpression of OsERF83, a Vascular Tissue-Specific Transcription Factor Gene, Confers Drought Tolerance in Rice

Jung, Se Eun; Bang, Seung Woon; Kim, Sung Hwan; Seo, Jun Sung; Yoon, Ho-Bin; Kim, Youn Shic; Kim, Ju-Kon

doi:10.3390/ijms22147656

Cited by 37 publications

(19 citation statements)

References 64 publications

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“…OsGGPS , OsTPS3 , and OsTPS23 were upregulated four‐ to fivefold in T_CS, suggesting a role for terpenoids in DREB1a‐mediated stress tolerance. Jung et al ( 2021 ) found induction of OsTPS3 as part of the mechanism associated with improved drought tolerance in rice. Phenylpropanoid pathway genes OsCCR and OsC4H that encode cinnamoyl‐CoA reductase and cinnamate 4‐hydroxylase, respectively, were upregulated >6‐fold in T_CS (Table 2 ).…”

Section: Resultsmentioning

confidence: 99%

“…Secondary metabolitesSeveral genes involved in secondary metabolite production were markedly upregulated in T_CS that include genes encoding geranylgeranyl pyrophosphate synthase (GGPS), terpene synthases (TPS), phenylpropanoid (lignin) pathway, and polyamine oxidase (PAO). OsGGPS, OsTPS3, and OsTPS23 were upregulated four-to fivefold in T_CS, suggesting a role for terpenoids in DREB1a-mediated stress tolerance Jung et al (2021). found induction of OsTPS3 as part of the mechanism associated with improved drought tolerance in rice.…”

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confidence: 84%

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Phenotypic and transcriptomic analysis reveals early stress responses in transgenic rice expressing Arabidopsis DREB1a

et al. 2022

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Overexpression of Arabidopsis dehydration response element binding 1a ( DREB1a ) is a well‐known approach for developing salinity, cold and/or drought stress tolerance. However, understanding of the genetic mechanisms associated with DREB1a expression in rice is generally limited. In this study, DREB1a ‐associated early responses were investigated in a transgenic rice line harboring cold‐inducible DREB1a at a gene stacked locus. Although the function of other genes in the stacked locus was not relevant to stress tolerance, this study demonstrates DREB1a can be co‐localized with other genes for multigenic trait enhancement. As expected, the transgenic lines displayed improved tolerance to salinity stress and water withholding as compared with non‐transgenic controls. RNA sequencing and transcriptome analysis showed upregulation of complex transcriptional networks and metabolic reprogramming as DREB1a expression led to the upregulation of multiple transcription factor gene families, suppression of photosynthesis, and induction of secondary metabolism. In addition to the detection of previously described mechanisms such as production of protective molecules, potentially novel pathways were also revealed. These include jasmonate, auxin, and ethylene signaling, induction of JAZ and WRKY regulons, trehalose synthesis, and polyamine catabolism. These genes regulate various stress responses and ensure timely attenuation of the stress signal. Furthermore, genes associated with heat stress response were downregulated in DREB1a expressing lines, suggesting antagonism between heat and dehydration stress response pathways. In summary, through a complex transcriptional network, multiple stress signaling pathways are induced by DREB1a that presumably lead to early perception and prompt response toward stress tolerance as well as attenuation of the stress signal to prevent deleterious effects of the runoff response.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 84%

Phenotypic and transcriptomic analysis reveals early stress responses in transgenic rice expressing Arabidopsis DREB1a

et al. 2022

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“…OsGGPS, OsTPS3 and OsTPS23 were upregulated 4 -5-fold in T_CS, suggesting a role for terpenoids in DREB1a mediated abiotic stress tolerance. Jung et al (2021) found induction of OsTPS3 as part of the mechanism associated with improved drought tolerance. Phenylpropanoid pathway genes, OsCCR and OsC4H that encode Cinnamoyl-CoA reductase and Cinnamate 4-hydroxylase, respectively, were upregulated > 6-fold in T_CS (Table 2).…”

Section: Secondary Metabolitesmentioning

confidence: 96%

Phenotypic and transcriptomic analysis reveals early stress responses in transgenic rice expressing Arabidopsis DREB1a

Berchembrock

Pathak

Maurya

et al. 2022

Preprint

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Overexpression of Arabidopsis Dehydration Response Element Binding 1a (DREB1a) is a well-known approach for developing salinity, cold and/or drought stress tolerance. However, understanding of the genetic mechanisms associated with DREB1a expression in rice is generally limited. In this study, DREB1a associated early responses were investigated in a transgenic rice line harboring cold-inducible DREB1a at a gene stacked locus. While the function of other genes in the stacked locus was not relevant to stress tolerance, this study demonstrates DREB1a can be colocalized with other genes for multigenic trait enhancement. As expected, the transgenic lines displayed improved tolerance to salinity stress and water withholding when compared to non-transgenic controls. RNA sequencing and transcriptome analysis showed upregulation of complex transcriptional networks and metabolic reprogramming as DREB1a expression led to the upregulation of multiple transcription factor gene families, suppression of photosynthesis and induction of secondary metabolism. In addition to the detection of previously described mechanisms such as production of protective molecules, potentially novel pathways were also revealed. These include jasmonate, auxin, and ethylene signaling, induction of JAZ and WRKY regulons, trehalose synthesis and polyamine catabolism. These genes regulate various stress responses and ensure timely attenuation of the stress signal. Furthermore, genes associated with heat stress response were downregulated in DREB1a overexpressing lines, suggesting antagonism between heat and dehydration stress pathways. In summary, through a complex transcriptional network, multiple stress signaling pathways are induced by DREB1a that presumably lead to early perception and rapid response towards stress tolerance as well as attenuation of the signal to prevent deleterious effects of the runoff response.

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“…In rice, Sub1A and SNORKEL1/2 modulate flooding tolerance via two different physiological mechanisms [ 60 , 61 , 62 , 63 ]. Overexpression of OsDREB1s , OsDREB2A , OsEREBP1 , OsERF48 , OsERF71 , OsLG3 , OsERF83 , OsERF101 , OsERF115 , and OsERF4a ( OsERF3 ) improved drought tolerance in rice, through activating the jasmonate and abscisic acid (ABA) signaling pathways or modulating root architecture [ 57 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 ]. By contrast, OsEBP89 , OsERF109 , OsDERF1 , OsERF3, and OsAP2-39 negatively regulated rice drought tolerance [ 70 , 79 , 80 , 81 , 82 ].…”

Section: Introductionmentioning

confidence: 99%

ERF Transcription Factor OsBIERF3 Positively Contributes to Immunity against Fungal and Bacterial Diseases but Negatively Regulates Cold Tolerance in Rice

Hong

Wang

Gao

et al. 2022

IJMS

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We previously showed that overexpression of the rice ERF transcription factor gene OsBIERF3 in tobacco increased resistance against different pathogens. Here, we report the function of OsBIERF3 in rice immunity and abiotic stress tolerance. Expression of OsBIERF3 was induced by Xanthomonas oryzae pv. oryzae, hormones (e.g., salicylic acid, methyl jasmonate, 1-aminocyclopropane-1-carboxylic acid, and abscisic acid), and abiotic stress (e.g., drought, salt and cold stress). OsBIERF3 has transcriptional activation activity that depends on its C-terminal region. The OsBIERF3-overexpressing (OsBIERF3-OE) plants exhibited increased resistance while OsBIERF3-suppressed (OsBIERF3-Ri) plants displayed decreased resistance to Magnaporthe oryzae and X. oryzae pv. oryzae. A set of genes including those for PRs and MAPK kinases were up-regulated in OsBIERF3-OE plants. Cell wall biosynthetic enzyme genes were up-regulated in OsBIERF3-OE plants but down-regulated in OsBIERF3-Ri plants; accordingly, cell walls became thicker in OsBIERF3-OE plants but thinner in OsBIERF3-Ri plants than WT plants. The OsBIERF3-OE plants attenuated while OsBIERF3-Ri plants enhanced cold tolerance, accompanied by altered expression of cold-responsive genes and proline accumulation. Exogenous abscisic acid and 1-aminocyclopropane-1-carboxylic acid, a precursor of ethylene biosynthesis, restored the attenuated cold tolerance in OsBIERF3-OE plants while exogenous AgNO3, an inhibitor of ethylene action, significantly suppressed the enhanced cold tolerance in OsBIERF3-Ri plants. These data demonstrate that OsBIERF3 positively contributes to immunity against M. oryzae and X. oryzae pv. oryzae but negatively regulates cold stress tolerance in rice.

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Overexpression of OsERF83, a Vascular Tissue-Specific Transcription Factor Gene, Confers Drought Tolerance in Rice

Cited by 37 publications

References 64 publications

Phenotypic and transcriptomic analysis reveals early stress responses in transgenic rice expressing Arabidopsis DREB1a

Phenotypic and transcriptomic analysis reveals early stress responses in transgenic rice expressing Arabidopsis DREB1a

Phenotypic and transcriptomic analysis reveals early stress responses in transgenic rice expressing Arabidopsis DREB1a

ERF Transcription Factor OsBIERF3 Positively Contributes to Immunity against Fungal and Bacterial Diseases but Negatively Regulates Cold Tolerance in Rice

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