Overexpression of OsMYB4P, an R2R3-type MYB transcriptional activator, increases phosphate acquisition in rice

Yang, Won Tae; Baek, Dongwon; Yun, Dae‐Jin; Hwang, Woon Ha; Park, Dong Soo; Nam, Min Hee; Chung, Eun‐Sung; Chung, Young Soo; Yi, Young-Byung; Kim, Doh Hoon

doi:10.1016/j.plaphy.2014.02.024

Cited by 57 publications

(68 citation statements)

References 40 publications

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“…The expression of the rice OsMYB4P gene encoding an R2R3-type MYELOBLASTOSIS (MYB) protein is induced in the wild-type root after 7 d of Pi deprivation. Interestingly, when overexpressed, this gene increases the PR length independent of the Pi supply (Yang et al, 2014). This is reminiscent of results obtained with OsMYB2P-1, a closely related MYB transcription factor gene with expression that is also induced in roots of seedlings without phosphate (Dai et al, 2012).…”

Section: Pr Response In Monocot Speciessupporting

confidence: 65%

“…2 and 3), possibly because their seeds contain more abundant phosphorus reserves (Calderón-Vázquez et al, 2011). For example, low Pi slightly stimulates growth of the PR in maize (Zea mays; Li et al, 2012) and rice 'Japonica' (Zhou et al, 2008;Dai et al, 2012), although some reports are contradictory (for example, Yang et al, 2014). This may be attributed to differences in crop cultivars and experimental conditions.…”

Section: Pr Response In Monocot Speciesmentioning

confidence: 99%

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Root Architecture Responses: In Search of Phosphate

Péret¹,

Desnos²,

Jost³

et al. 2014

Plant Physiol.

225

141

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Soil phosphate represents the only source of phosphorus for plants and, consequently, is its entry into the trophic chain. This major component of nucleic acids, phospholipids, and energy currency of the cell (ATP) can limit plant growth because of its low mobility in soil. As a result, root responses to low phosphate favor the exploration of the shallower part of the soil, where phosphate tends to be more abundant, a strategy described as topsoil foraging. We will review the diverse developmental strategies that can be observed among plants by detailing the effect of phosphate deficiency on primary and lateral roots. We also discuss the formation of cluster roots: an advanced adaptive strategy to cope with low phosphate availability observed in a limited number of species. Finally, we will put this work into perspective for future research directions.

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Section: Pr Response In Monocot Speciessupporting

confidence: 65%

Section: Pr Response In Monocot Speciesmentioning

confidence: 99%

Root Architecture Responses: In Search of Phosphate

Péret¹,

Desnos²,

Jost³

et al. 2014

Plant Physiol.

225

141

View full text Add to dashboard Cite

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“…Considering the ways and means to increase P remobilization from roots in rice, achieving a greater size of the root system might be an option, several genes associated with enhanced root growth of rice under P deficiency have been identified (Gamuyao et al, ; Yang et al, ; Y. M. Zhang et al, ). A quantitative trait locus for P‐deficiency tolerance, Pup1 , was identified in the Indica type traditional rice variety Kasalath (Wissuwa & Ae, ; Wissuwa, Yano, & Ae, ).…”

Section: Roots: Potential Of the Hidden Half For Remobilization Of P mentioning

confidence: 99%

“…The responsible gene was designated as phosphorus‐starvation tolerance 1 ( PSTOL1 ), which acts as an early root growth enhancer thereby enabling plants to acquire more Pi from soil (Gamuyao et al, ). Considering the other genes that influence root growth of rice, both OsMYB4P encoding an R2R3‐type MYELOBLASTOSIS protein (Yang et al, ) and nutrition response and root growth (Y. M. Zhang et al, ) have been identified. Overexpression of OsMYB4P enhances primary root length of rice, irrespective of Pi supply (Yang et al, ), whereas knockdown lines of nutrition response and root growth enhance rice root growth under Pi‐deficient conditions (Y. M. Zhang et al, ).…”

Section: Roots: Potential Of the Hidden Half For Remobilization Of P mentioning

confidence: 99%

Molecular mechanisms underpinning phosphorus‐use efficiency in rice

Dissanayaka

Plaxton

Lambers

et al. 2018

Plant Cell & Environment

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Orthophosphate (H PO , Pi) is an essential macronutrient integral to energy metabolism as well as a component of membrane lipids, nucleic acids, including ribosomal RNA, and therefore essential for protein synthesis. The Pi concentration in the solution of most soils worldwide is usually far too low for maximum growth of crops, including rice. This has prompted the massive use of inefficient, polluting, and nonrenewable phosphorus (P) fertilizers in agriculture. We urgently need alternative and more sustainable approaches to decrease agriculture's dependence on Pi fertilizers. These include manipulating crops by (a) enhancing the ability of their roots to acquire limiting Pi from the soil (i.e. increased P-acquisition efficiency) and/or (b) increasing the total biomass/yield produced per molecule of Pi acquired from the soil (i.e. increased P-use efficiency). Improved P-use efficiency may be achieved by producing high-yielding plants with lower P concentrations or by improving the remobilization of acquired P within the plant so as to maximize growth and biomass allocation to developing organs. Membrane lipid remodelling coupled with hydrolysis of RNA and smaller P-esters in senescing organs fuels P remobilization in rice, the world's most important cereal crop.

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“…Expression of OsMYB1R1 in (A) NIL hd1 (with a nonfunctional Hd1 allele) and its WT Nipponbare, (B) NIL dth8 (with a nonfunctional DTH8 allele) and its WT Asominori, and (C) NIL Ghd7 (with a functional Ghd7 allele), and NIL ghd7 (with a nonfunctional Ghd7 allele). nutrient mobilization [51,52], and anther development [53,54]. To date, rice MYB TFs regulating flowering time have not been reported.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of OsMYB1R1–VP64 fusion protein increases grain yield in rice by delaying flowering time

Wang

Zhu

et al. 2016

FEBS Letters

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Edited by Ulf-Ingo Fl€ ugge VP64 is widely used as a transcriptional activator to investigate the biological function of genes, but its potential for application in genetic improvement of crops has not been fully investigated. Here, we characterized an OsMYB1R1-VP64 fusion protein that enhanced the grain yield of rice cultivar 'Kita-ake' by 35%. OsMYB1R1-VP64 regulated grain yield of transgenic plants mainly by extending the vegetative growth stage. Further analysis indicated that OsMYB1R1-VP64 delayed flowering by down-regulating expression of Heading date 3a (Hd3a) and RICE FLOWERING LOCUST 1 (RFT1) through repression of Early heading date 1 (Ehd1). The expression pattern of OsMYB1R1 was constitutive and rhythmically controlled. OsMYB1R1 protein had transcriptional activation activity and was localized to the nucleus. Our findings suggest that the OsMYB1R1-VP64 construct can be used to increase grain yield in rice.Keywords: flowering time; grain yield; VP64 Rice (Oryza sativa L.) is one of the most important food crops in the world, and is the staple food for more than 75% of the population in Asia [1]. With increasing population and decreasing arable land area more productive varieties are needed to address food shortages. The yield of a rice variety is determined by three important factors, namely effective panicle number per plant, grain number per panicle, and 1000-grain weight. These in turn are affected by genotype, environmental conditions (physical environment and agronomy), and their interactions. The key genetic factors are tiller number, flowering time, fertility, and seed setting rate. As the transition point, flowering time plays a key role in coordination between vegetative and reproductive growth [2]. Vegetative growth sets the basis of reproductive growth, which involves the formation, quality, and nutritive accumulation of reproductive organs. Flowering time also affects crop yield by enabling crops to gain the maximum benefit from the prevailing conditions [3,4]. Therefore, control and optimization of flowering time has been under intense scrutiny by breeders and agronomists for a long time [5].

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Overexpression of OsMYB4P, an R2R3-type MYB transcriptional activator, increases phosphate acquisition in rice

Cited by 57 publications

References 40 publications

Root Architecture Responses: In Search of Phosphate

Root Architecture Responses: In Search of Phosphate

Molecular mechanisms underpinning phosphorus‐use efficiency in rice

Overexpression of OsMYB1R1–VP64 fusion protein increases grain yield in rice by delaying flowering time

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