Overexpression of OsPIN2 Regulates Root Growth and Formation in Response to Phosphate Deficiency in Rice

Sun, Huwei; Guo, Xiaoli; Xu, Feifei; Wu, Daxia; Zhang, Xuhong; Lou, Manman; Luo, Feifei; Xu, Guohua; Zhang, Yali

doi:10.3390/ijms20205144

Cited by 32 publications

(19 citation statements)

References 45 publications

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“…OsPIN2 overexpression lines showed shorter seminal root length and lower auxin accumulation in the root cap, suggesting that OsPIN2 might inhibit seminal root elongation by reducing auxin accumulation in the root cap. Under normal nutritional conditions, overexpression of OsPIN2 increases auxin distribution in the root epidermis, resulting in greater root hair formation but less lateral root development [102]. The plant height of the ospin9 mutant was significantly lower than that of WT, and the number of adventitious roots was significantly reduced [93].…”

Section: Auxin Transporters Is Involved In Root Development In Ricementioning

confidence: 99%

Advances in the Biological Functions of Auxin Transporters in Rice

Feng

Bayaer

2022

Agriculture

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Auxin is the earliest discovered plant hormone, which plays important roles in each aspect of plant growth and development. There are two main pathways for auxin to be transported from the synthetic site (such as young leaves and terminal buds) to the active site. First, auxin is transported over long distances through phloem in an unfixed direction throughout the whole plant. Second, short-distance polar transport between cells requires the participation of auxin carriers, including unidirectional transport from stem tip to root and local unidirectional transport between tissues. Polar transport is critical to the establishment and maintenance of the auxin concentration gradient, which specifically regulates plant growth and development and responds to environmental changes. In this article, we reviewed the research progress of auxin transporters AUX1/LAX, PIN, and ABCB families, and some potential auxin transporters in rice growth and development, which provide information for the interpretation of biological functions of auxin polar transporter families and lay a foundation for the genetic improvement of important agronomic traits in rice.

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Section: Auxin Transporters Is Involved In Root Development In Ricementioning

confidence: 99%

Advances in the Biological Functions of Auxin Transporters in Rice

Feng

Bayaer

2022

Agriculture

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“…osaux4 mutants were insensitive to Pi starvation Phosphate is an essential macronutrient in plant growth, and auxin signaling has been reported to participate in the root architecture alteration caused by Pi deficiency (Al-Ghazi et al, 2003;Niu et al, 2013). To date, some genes related to auxin signaling have been reported to be involved in the Pi starvation response (Shen et al, 2013;Giri et al, 2018;Sun et al, 2019). To investigate whether OsAUX4 is also correlated with the Pi starvation response, we first evaluated the OsAUX4 expression pattern under control (Pi sufficient) and -Pi (Pi starvation) conditions, using GUS staining of ProOsAUX4:GUS transgenic rice and qRT-PCR in rice roots.…”

Section: Decreased Cell Division Activity Of Root Apical Meristem and Downregulation Of Cell Cycle Gene Expression In Osaux4 Mutantsmentioning

confidence: 99%

Primary root and root hair development regulation by OsAUX4 and its participation in the phosphate starvation response

Gao

et al. 2021

JIPB

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Among the five members of AUX1/LAX genes coding for auxin carriers in rice, only OsAUX1 and OsAUX3 have been reported. To understand the function of the other AUX1/LAX genes, two independent alleles of osaux4 mutants, osaux4-1 and osaux4-2, were constructed using the CRISPR/Cas9 editing system. Homozygous osaux4-1 or osaux4-2 exhibited shorter primary root (PR) and longer root hair (RH) compared to the wild-type Dongjin (WT/DJ), and lost response to indoleacetic acid (IAA) treatment. OsAUX4 is intensively expressed in roots and localized on the plasma membrane, suggesting that OsAUX4 might function in the regulation of root development. The decreased meristem cell division activity and the downregulated expression of cell cycle genes in root apices of osaux4 mutants supported the hypothesis that OsAUX4 positively regulates PR elongation. OsAUX4 is expressed in RH, and osaux4 mutants showing longer RH compared to WT/DJ implies that OsAUX4 negatively regulates RH development. Furthermore, osaux4 mutants are insensitive to Pi starvation (-Pi) and OsAUX4 effects on the -Pi response is associated with altered expression levels of Pi starvation-regulated genes, and auxin distribution/ contents. This study revealed that OsAUX4 not only regulates PR and RH development but also plays a regulatory role in crosstalk between auxin and -Pi signaling.

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“…For example, low phosphorus treatment increased root length and root hair density of wild rice seeds (Giri et al, 2018; Jose ne and Matthias, 2016). However, overexpression of OsPIN2 resulted in the loss of sensitivity of roots to phosphorus de ciency, and the root length of rice was shortened by increasing the distribution of auxin in root epidermis (Sun et al, 2019). Similarly, overexpression of SAPK10 (Stress/ABA-activated protein kinase 10) resulted in longer root hairs in rice, while overexpression of OsABI2 (OsABI-Like 2) resulted in weakened ABA signal transduction and shorter root hairs in rice plants (Wang et al, 2017).…”

Section: Sbbhlh85 Different From Its Homologs In Other Species Is a N...mentioning

confidence: 99%

SbbHLH85, a bHLH member, modulates resilience to salt stress by regulating root hair growth in sorghum

Song

Sui

et al. 2021

Theor Appl Genet

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Introduction: bHLH family proteins play an important role in plant stress response. However, the molecular mechanism regulating salt response of bHLH is largely unknown.Materials and Methods: Here, we investigated the function and regulating mechanism of the sweet sorghum SbbHLH85 during salt stress.Results: Results show that SbbHLH85, different from its homologs in other species, is a new atypical bHLH transcription factor and is a key gene for root development in sweet sorghum. Knockout of SbbHLH85 in sorghum by CRISPR-Cas9 results in the inhibition of root development. Overexpression of SbbHLH85 resulted in signi cantly increased number and length of root hairs by participating in ABA and auxin signaling pathways, which can increase the absorption of Na + . While SbbHLH85 plays a negative regulatory role in salt tolerance of sorghum. Through screening yeast two-hybrid library, we identi ed a potential interaction partner of SbbHLH85 that is phosphate transporter chaperone PHF1, which modulates the distribution of phosphate. Both yeast two-hybrid and BiFC experiments con rmed interaction between SbbHLH85 and PHF1.Discussion: Based on these results, we suggest that the increase of Na + content and the decrease of Pi content result in the salt sensitivity of transgenic sorghum.

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Overexpression of OsPIN2 Regulates Root Growth and Formation in Response to Phosphate Deficiency in Rice

Cited by 32 publications

References 45 publications

Advances in the Biological Functions of Auxin Transporters in Rice

Advances in the Biological Functions of Auxin Transporters in Rice

Primary root and root hair development regulation by OsAUX4 and its participation in the phosphate starvation response

SbbHLH85, a bHLH member, modulates resilience to salt stress by regulating root hair growth in sorghum

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