Overexpression of OsSAP16 Regulates Photosynthesis and the Expression of a Broad Range of Stress Response Genes in Rice (Oryza sativa L.)

Abstract

This study set out to identify and characterize transcription factors regulating photosynthesis in rice. Screening populations of rice T-DNA activation lines led to the identification of a T-DNA mutant with an increase in intrinsic water use efficiency (iWUE) under well-watered conditions. Flanking sequence analysis showed that the T-DNA construct was located upstream of LOC_Os07g38240 (OsSAP16) encoding for a stress-associated protein (SAP). A second mutant identified with activation in the same gene exhibited the same phenotype; expression of OsSAP16 was shown to be enhanced in both lines. There were no differences in stomatal development or morphology in either of these mutants, although overexpression of OsSAP16 reduced stomatal conductance. This phenotype limited CO2 uptake and the rate of photosynthesis, which resulted in the accumulation of less biomass in the two mutants. Whole transcriptome analysis showed that overexpression of OsSAP16 led to global changes in gene expression consistent with the function of zinc-finger transcription factors. These results show that the gene is involved in modulating the response of rice to drought stress through regulation of the expression of a set of stress-associated genes.

Response of Eustoma Leaf Phenotype and Photosynthetic Performance to LED Light Quality

“…In particular, the higher A observed supported the growth of Eustoma leaves from our results [28]. It is well established that g s correlates with the A [62] and a lower E is also related to a reduction in g s [63]. The higher E under the B and W LEDs led to an increase in fresh biomass and a change in leaf shape compared with the R LEDs at higher PPFDs (Figure 1(A4),C; Figure 2C).…”

Identification of a Major QTL and Candidate Gene Analysis of Salt Tolerance at the Bud Burst Stage in Rice (Oryza sativa L.) Using QTL-Seq and RNA-Seq

“…Among them, OsSAP16 showed a 1 bp difference in the promoter region between IR36 and Weiguo and was significantly associated with the salt-tolerant phenotype. This gene encoded a C2H2-type zinc finger protein and would be induced under drought stress (Wang et al 2016). Furthermore, according to the transcriptome analysis and qRT-PCR results, the candidate gene OsSAP16 was significantly down-regulated after salt stress in IR vs. TIR (Fig.…”

“…Therefore, Os07g0569700 was considered as the most possible functional gene underlying qRSL7. The candidate gene Os07g0569700 has been studied and named as OsSAP16 in a prior study (Wang et al 2016). This gene encodes a stress-associated protein and its expression has been shown to increase under drought stress.…”

A wheat gene TaSAP17-D encoding an AN1/AN1 zinc finger protein improves salt stress tolerance in transgenic Arabidopsis

“…Overexpression of OsSAP16 can regulate the expression of a set of stress-associated genes, including pathogenesis-related (PR) proteins, signaling proteins, secondary metabolism proteins, MYB transcription factor, and peroxidases (Wang et al 2016). We found that the amino acid sequence of OsSAP16 showed the highest homology with TaSAP17 and interestingly, the overexpression of TaSAP17-D upregulated a broad range of stress responsive genes.…”