Overexpression of osteoprotegerin promotes preosteoblast differentiation to mature osteoblasts

Abstract

Objective: The hypothesis of the present study is that overexpression of osteoprotegerin (OPG) promotes preosteoblast maturation. Materials and Methods: The preosteoblast cell line MC3T3-E1 was transfected with OPG overexpression. OPG expression was confirmed by enzyme-linked immunosorbent assay (ELISA) and Western blot. Changes in the transcription factors in OPG-expressing cells were assessed by real-time polymerase quantitative polymerase chain reaction (RT-qPCR). Alkaline phosphate (ALP) expression was measured by ELISA. Results: The success of stable transfection of MC3T3-E1 cells with OPG overexpression was confirmed by MoFlow sorting followed by G418 selection. RT-qPCR showed that expression of RunX2, the most important osteoblast differentiation controlling factor, was suppressed. Smad1 and Akt1, as well as ALP, were upregulated in the OPG overexpressing cells. Conclusion:Results from the present study provide evidence that overexpression of OPG in preosteoblasts promotes its differentiation into mature osteoblasts. (Angle Orthod. 2011;81:100-106.)

Interleukin‐33 could play an important role in the pathogenesis of periodontitis

“…However, there were greater numbers of osteoblasts in the LO group in the present study than in the study by Demirer et al (20). Previous studies (22,23) have shown that over-expression of OPG stimulates the differentiation of pre-osteoblasts into mature osteoblasts. The increased number of osteoblasts observed in the present study might have been related to increased expression of OPG in the LO group.…”

“…. Previous studies have shown that over‐expression of OPG stimulates the differentiation of pre‐osteoblasts into mature osteoblasts. The increased number of osteoblasts observed in the present study might have been related to increased expression of OPG in the LO group.…”

Jagged1 inhibits osteoprotegerin expression by human periodontal ligament cells

“…OPG (40 ng/mL) primed human mesenchymal stem cells demonstrated increased osteocalcin expression upon osteogenic induction (31). In addition, OPG overexpression in murine preosteoblasts resulted in increased ALP activity (32). In contrast, it has been shown that OPG (0.1-10 ng/mL) inhibited osteogenic differentiation in rat vascular smooth muscle cells, as determined by decreased ALP activity and mineralization (21).…”

Association of Serum Phosphate and Related Factors in ESRD-Related Vascular Calcification

“…A signal from AS plaque induces expression of potent osteogenic differentiation factor BMP-2a, with bone matrix and calcium hydroxyapatite crystals deposition in the arterial wall cells [41, 42]. Osteoprotegerin (OPG) [43, 44] inactivates the osteoclasts by blocking RANK activation. In dialysis patients, serum OPG levels are associated and predict progressive vascular calcification especially when CRP level is increased.…”