Overexpression of PagSTOMAGEN, a Positive Regulator of Stomatal Density, Promotes Vegetative Growth in Poplar

Xia, Yufei; Du, Kang; Ling, Aoyu; Wu, Wenqi; Li, Jiang; Kang, Xiangyang

doi:10.3390/ijms231710165

Cited by 10 publications

(8 citation statements)

References 77 publications

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“…RT-qPCR assays were performed using TransStart Top Green qPCR SuperMix (TRANSGEN, China, Cat. AQ132-22) [ 50 ]. Three biological replicates were performed for each tissue.…”

Section: Methodsmentioning

confidence: 99%

Effect of a suitable treatment period on the genetic transformation efficiency of the plant leaf disc method

et al. 2023

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Background Agrobacterium tumefaciens-mediated leaf disc genetic transformation is an important way to achieve transgenics or gene editing. Ensuring stable and efficient genetic transformation is still an important problem in modern biology. It is assumed that the difference in the development status of genetic transformation cells of receptor materials is the main reason for the difference and instability of genetic transformation efficiency; the stable and efficient genetic transformation rate can be obtained by defining the appropriate treatment period of the receptor material and applying genetic transformation in a timely manner. Results Based on these assumptions, we studied and established an efficient and stable Agrobacterium-mediated plant transformation system with hybrid poplar (Populus alba × Populus glandulosa, 84 K) leaves, stem segments and tobacco leaves as the research objects. There were differences in the development process of leaf bud primordial cells from different explants, and the genetic transformation efficiency was significantly related to the cell development stage of the in vitro cultured materials. Among them, the genetic transformation rate of poplar and tobacco leaves was the highest on the 3rd and 2nd day of culture, reaching 86.6% and 57.3%, respectively. The genetic transformation rate of poplar stem segments was the highest on the 4th day of culture, reaching 77.8%. The best treatment period was from the development of leaf bud primordial cells to the S phase of the cell cycle. The number of cells detected using flow cytometry and 5-ethynyl-2ʹ-deoxyuridine (EdU) staining, the expression of cell cycle-related protein CDKB1; 2, CDKD1; 1, CYCA3; 4, CYCD1; 1, CYCD3; 2, CYCD6; 1, and CYCH; 1 of explants, and morphological changes of explants can be used as indicators to determine the appropriate treatment period for genetic transformation. Conclusions Our study provides a new and universal set of methods and characteristics to identify the S phase of the cell cycle and apply genetic transformation treatments at the appropriate time. Our results are of great significance for improving the efficiency and stability of plant leaf disc genetic transformation.

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“…RT-qPCR assays were performed using TransStart Top Green qPCR SuperMix (TRANSGEN, China, Cat. AQ132-22) [ 50 ]. Three biological replicates were performed for each tissue.…”

Section: Methodsmentioning

confidence: 99%

Effect of a suitable treatment period on the genetic transformation efficiency of the plant leaf disc method

et al. 2023

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“…Overexpression of PdERECTA and PdEPFL6 also resulted in decreased stomatal density and increased drought tolerance without a significant reduction in biomass accumulation under non-limiting water conditions. However, when the positive regulator of stomatal development STOMAGEN was overexpressed in the poplar hybrid clone 84K, the plants displayed increased stomatal density and conductance, higher net photosynthetic rate, and higher vegetative growth [ 60 ]. In other cases, the complexity of the regulatory circuits involved in the control of stomatal differentiation makes it difficult to examine fully the effects of their perturbation [ 61 , 62 ].…”

Section: Identification Of Genes and Pathways Modulating Stomatal Dev...mentioning

confidence: 99%

“…Overall, published works suggest that reduction of stomatal density is a promising strategy for breeding or biotechnological programs aimed at obtaining drought resilient poplar genotypes [ 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 ]. However, field-based trials remain desirable, as some observations made in natural conditions are not fully in agreement with those in confined environments.…”

Section: Identification Of Genes and Pathways Modulating Stomatal Dev...mentioning

confidence: 99%

Responses to Drought Stress in Poplar: What Do We Know and What Can We Learn?

Rosso¹,

Cantamessa²,

Bergante³

et al. 2023

Life

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Poplar (Populus spp.) is a high-value crop for wood and biomass production and a model organism for tree physiology and genomics. The early release, in 2006, of the complete genome sequence of P. trichocarpa was followed by a wealth of studies that significantly enriched our knowledge of complex pathways inherent to woody plants, such as lignin biosynthesis and secondary cell wall deposition. Recently, in the attempt to cope with the challenges posed by ongoing climate change, fundamental studies and breeding programs with poplar have gradually shifted their focus to address the responses to abiotic stresses, particularly drought. Taking advantage from a set of modern genomic and phenotyping tools, these studies are now shedding light on important processes, including embolism formation (the entry and expansion of air bubbles in the xylem) and repair, the impact of drought stress on biomass yield and quality, and the long-term effects of drought events. In this review, we summarize the status of the research on the molecular bases of the responses to drought in poplar. We highlight how this knowledge can be exploited to select more tolerant genotypes and how it can be translated to other tree species to improve our understanding of forest dynamics under rapidly changing environmental conditions.

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“…The genes of the EPF / EPFL family indirectly affect stomatal development, for example EPFL9 positively regulates stomatal density, and EPF1 and EPF2 negatively regulate stomatal density. Studies have shown that as ligands, EPFL9 , EPF1 , and EPF2 can directly bind to receptors such as TMM/ERECTA (Hara et al ., 2007; Hunt & Gray, 2009; Lee et al ., 2015), thereby affecting the expression of downstream SPCH or MUTE (Pillitteri & Torii, 2012), and ultimately regulating stomatal development (Lee et al ., 2015; Xia et al ., 2022). A single ERECTA family (ERf) without a TMM recognize EPFL4 and EPFL6 of the EPF / EPFL family, and they can reduce stomatal density when combined with ERf receptors (Abrash et al ., 2011; Lin et al ., 2017; Jiao et al ., 2022).…”

Section: Introductionmentioning

confidence: 99%

MYC2 regulates stomatal density and water use efficiency via targeting EPF2/EPFL4/EPFL9 in poplar

Xia,

Jiang,

et al. 2024

New Phytologist

Self Cite

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Summary Although polyploid plants have lower stomatal density than their diploid counterparts, the molecular mechanisms underlying this difference remain elusive. Here, we constructed a network based on the triploid poplar transcriptome data and triple‐gene mutual interaction algorithm and found that PpnMYC2 was related to stomatal development‐related genes PpnEPF2, PpnEPFL4, and PpnEPFL9. The interactions between PpnMYC2 and PagJAZs were experimentally validated. PpnMYC2‐overexpressing poplar and Arabidopsis thaliana had reduced stomatal density. Poplar overexpressing PpnMYC2 had higher water use efficiency and drought resistance. RNA‐sequencing data of poplars overexpressing PpnMYC2 showed that PpnMYC2 promotes the expression of stomatal density inhibitors PagEPF2 and PagEPFL4 and inhibits the expression of the stomatal density‐positive regulator PagEPFL9. Yeast one‐hybrid system, electrophoretic mobility shift assay, ChIP‐qPCR, and dual‐luciferase assay were employed to substantiate that PpnMYC2 directly regulated PagEPF2, PagEPFL4, and PagEPFL9. PpnMYC2, PpnEPF2, and PpnEPFL4 were significantly upregulated, whereas PpnEPFL9 was downregulated during stomatal formation in triploid poplar. Our results are of great significance for revealing the regulation mechanism of plant stomatal occurrence and polyploid stomatal density, as well as reducing stomatal density and improving plant water use efficiency by overexpressing MYC2.

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Overexpression of PagSTOMAGEN, a Positive Regulator of Stomatal Density, Promotes Vegetative Growth in Poplar

Cited by 10 publications

References 77 publications

Effect of a suitable treatment period on the genetic transformation efficiency of the plant leaf disc method

Effect of a suitable treatment period on the genetic transformation efficiency of the plant leaf disc method

Responses to Drought Stress in Poplar: What Do We Know and What Can We Learn?

MYC2 regulates stomatal density and water use efficiency via targeting EPF2/EPFL4/EPFL9 in poplar

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