Overexpression of pEGF improved the gut protective function of Clostridium butyricum partly through STAT3 signal pathway

Ma, Miaopeng; Zhao, Zitong; Liang, Qiao‐Mei; Shen, Haokun; Zhao, Zengjue; Chen, Ziyang; He, Rongxiao; Feng, Saixiang; Cao, Ding; Gan, Guanhua; Ye, Hejia; Qiu, Weihong; Deng, Jinbo; Ming, Feiping; Jia, Junhao; Sun, Chongjun; Li, Jiayi; Zhang, Linghua

doi:10.1007/s00253-021-11472-y

Cited by 13 publications

(9 citation statements)

References 79 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous in vivo and in vitro study revealed that CBM 588 would be effective to inhibit the proliferations of bacteria and can cause gastrointestinal infections [ 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 86 , 87 , 88 , 92 , 93 , 94 , 97 , 99 , 100 ]. Additionally, CBM 588 is a very safe drug since the bacteria has no toxin-producing genes and has been used clinically for over 70 years [ 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 ].…”

Section: Discussionmentioning

confidence: 99%

“…As a recent report suggested that C. butyricum alleviates intestinal injury through epidermal growth factor receptor (EGFR) [ 92 , 93 ], Ma et al [ 94 ] evaluated the inhibitory effects of the recombinant strain of C. butyricum overexpressing EGF. The recombinant strain significantly inhibited the growth of co-cultured S. aureus .…”

Section: Bacterial Gastrointestinal Infectionsmentioning

confidence: 99%

“…Compared with the wild-type strain, the recombinant strain increased the expression levels of intestinal formation-related genes ( Claudin-1 , GLUT-2 , SUC , GLP2R , EGFR ) and anti-inflammatory genes ( IL-10 ) in intestinal epithelial cells [ 94 ]. Hence, the secretory overexpression of pEGF in C. butyricum could upregulate the expression level of EGFR, consequently improving the intestinal protective functions of C. butyricum partly following STAT3 signal activation in IPECs, causing a positive loop.…”

Section: Bacterial Gastrointestinal Infectionsmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Clostridium butyricum on Gastrointestinal Infections

et al. 2022

View full text Add to dashboard Cite

Clostridium butyricum is a human commensal bacterium with beneficial effects including butyrate production, spore formation, increasing levels of beneficial bacteria, and inhibition of pathogenic bacteria. Owing to its preventive and ameliorative effects on gastrointestinal infections, C. butyricum MIYAIRI 588 (CBM 588) has been used as a probiotic in clinical and veterinary medicine for decades. This review summarizes the effects of C. butyricum, including CBM 588, on bacterial gastrointestinal infections. Further, the characteristics of the causative bacteria, examples of clinical and veterinary use, and mechanisms exploited in basic research are presented. C. butyricum is widely effective against Clostoridioides difficile, the causative pathogen of nosocomial infections; Helicobacter pylori, the causative pathogen of gastric cancer; and antibiotic-resistant Escherichia coli. Accordingly, its mechanism is gradually being elucidated. As C. butyricum is effective against gastrointestinal infections caused by antibiotics-induced dysbiosis, it can inhibit the transmission of antibiotic-resistant genes and maintain homeostasis of the gut microbiome. Altogether, C. butyricum is expected to be one of the antimicrobial-resistance (AMR) countermeasures for the One-health approach.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Bacterial Gastrointestinal Infectionsmentioning

confidence: 99%

Section: Bacterial Gastrointestinal Infectionsmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Clostridium butyricum on Gastrointestinal Infections

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Most of these studies involve modifying metabolic pathways to optimize production through gene overexpression or inactivation. From a health perspective, our laboratory constructed a porcine epidermal growth factor overexpressing CB to enhance its gut protective function [ 240 ]. However, it should be mentioned that the major hindrance to the application of engineered CB in health care is the paucity of viable genetic tools for CB, or in other words, genetic manipulation of CB is prone to failure.…”

Section: Modified Probiotics and Diseasesmentioning

confidence: 99%

“…A number of plasmids have been successfully applied to Clostridium spp. including plasmids such as pMTL007 [ 263 , 265 ] and pMTL82151 [ 240 , 266 ]. In terms of gene editing methods, Heap et al developed the ClosTron system in 2007 [ 267 ].…”

Section: Modified Probiotics and Diseasesmentioning

confidence: 99%

Native and Engineered Probiotics: Promising Agents against Related Systemic and Intestinal Diseases

Shen

Zhao

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

Intestinal homeostasis is a dynamic balance involving the interaction between the host intestinal mucosa, immune barrier, intestinal microecology, nutrients, and metabolites. Once homeostasis is out of balance, it will increase the risk of intestinal diseases and is also closely associated with some systemic diseases. Probiotics (Escherichia coli Nissle 1917, Akkermansia muciniphila, Clostridium butyricum, lactic acid bacteria and Bifidobacterium spp.), maintaining the gut homeostasis through direct interaction with the intestine, can also exist as a specific agent to prevent, alleviate, or cure intestinal-related diseases. With genetic engineering technology advancing, probiotics can also show targeted therapeutic properties. The aims of this review are to summarize the roles of potential native and engineered probiotics in oncology, inflammatory bowel disease, and obesity, discussing the therapeutic applications of these probiotics.

show abstract

Synthetic biology in multicellular organisms: Opportunities in nematodes

Kukhtar

Fussenegger

2023

Biotech & Bioengineering

View full text Add to dashboard Cite

Synthetic biology has mainly focused on introducing new or altered functionality in single cell systems: primarily bacteria, yeast, or mammalian cells. Here, we describe the extension of synthetic biology to nematodes, in particular the well‐studied model organism Caenorhabditis elegans, as a convenient platform for developing applications in a multicellular setting. We review transgenesis techniques for nematodes, as well as the application of synthetic biology principles to construct nematode gene switches and genetic devices to control motility. Finally, we discuss potential applications of engineered nematodes.

show abstract

Overexpression of pEGF improved the gut protective function of Clostridium butyricum partly through STAT3 signal pathway

Cited by 13 publications

References 79 publications

Effect of Clostridium butyricum on Gastrointestinal Infections

Effect of Clostridium butyricum on Gastrointestinal Infections

Native and Engineered Probiotics: Promising Agents against Related Systemic and Intestinal Diseases

Synthetic biology in multicellular organisms: Opportunities in nematodes

Contact Info

Product

Resources

About