Overexpression of Peroxiredoxin 6 Protects Neoplastic Cells against Apoptosis in Canine Haemangiosarcoma

Anwar, Sh.; Yanai, Tokuma; Sakai, Hiroki

doi:10.1016/j.jcpa.2016.05.002

Cited by 15 publications

(14 citation statements)

References 34 publications

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“…PRDX6 , also known as 1-Cys PRX, is a bifunctional enzyme [ 121 ] where it has a phospholipase A2 and glutathione peroxidase activities [ 121 ]. It is involved in tumour metastasis, proliferation and protection [ 121 ], playing both anti cytoprotective or cytoprotective roles [ 122 ]. PRDX6 was up-regulated in various cancers such as breast [ 121 ], lung [ 122 ], and oesophageal cancer [ 64 ].…”

Section: Discussionmentioning

confidence: 99%

“…It is involved in tumour metastasis, proliferation and protection [ 121 ], playing both anti cytoprotective or cytoprotective roles [ 122 ]. PRDX6 was up-regulated in various cancers such as breast [ 121 ], lung [ 122 ], and oesophageal cancer [ 64 ]. In the present study, PRDX6 was below threshold of detection, in the A2780 cisR cell line as compared to the level found in A2780 cells when A2780 was used as a reference, however, RDX6 was very sensitive to selected treatments, suggesting a direct association between promotion of apoptosis and PRDX6 level of expression.…”

Section: Discussionmentioning

confidence: 99%

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Sequenced Combinations of Cisplatin and Selected Phytochemicals towards Overcoming Drug Resistance in Ovarian Tumour Models

Althurwi

Beale

et al. 2020

IJMS

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In the present study, cisplatin, artemisinin, and oleanolic acid were evaluated alone, and in combination, on human ovarian A2780, A2780ZD0473R, and A2780cisR cancer cell lines, with the aim of overcoming cisplatin resistance and side effects. Cytotoxicity was assessed by MTT reduction assay. Combination index (CI) values were used as a measure of combined drug effect. MALDI TOF/TOF MS/MS and 2-DE gel electrophoresis were used to identify protein biomarkers in ovarian cancer and to evaluate combination effects. Synergism from combinations was dependent on concentration and sequence of administration. Generally, bolus was most synergistic. Moreover, 49 proteins differently expressed by 2 ≥ fold were: CYPA, EIF5A1, Op18, p18, LDHB, P4HB, HSP7C, GRP94, ERp57, mortalin, IMMT, CLIC1, NM23, PSA3,1433Z, and HSP90B were down-regulated, whereas hnRNPA1, hnRNPA2/B1, EF2, GOT1, EF1A1, VIME, BIP, ATP5H, APG2, VINC, KPYM, RAN, PSA7, TPI, PGK1, ACTG and VDAC1 were up-regulated, while TCPA, TCPH, TCPB, PRDX6, EF1G, ATPA, ENOA, PRDX1, MCM7, GBLP, PSAT, Hop, EFTU, PGAM1, SERA and CAH2 were not-expressed in A2780cisR cells. The proteins were found to play critical roles in cell cycle regulation, metabolism, and biosynthetic processes and drug resistance and detoxification. Results indicate that appropriately sequenced combinations of cisplatin with artemisinin (ART) and oleanolic acid (OA) may provide a means to reduce side effects and circumvent platinum resistance.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Sequenced Combinations of Cisplatin and Selected Phytochemicals towards Overcoming Drug Resistance in Ovarian Tumour Models

Althurwi

Beale

et al. 2020

IJMS

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“…PRDXs are a well-conserved family of thiol-dependent peroxidases that regulate the antioxidant defence system in almost all cell types by reducing hydrogen peroxide (H 2 O 2 ), peroxynitrite, and hydroperoxides. Abnormal function of PRDXs is associated with cancer, neoplasia, cardiovascular abnormalities, neurodegeneration, and male fertility 15 – 18 . In particular, an extensive literature search revealed that PRDXs play a critical role in the regulation of sperm function and male fertility 14 , 19 – 22 .…”

Section: Introductionmentioning

confidence: 99%

Peroxiredoxin activity is a major landmark of male fertility

Ryu

Kim

Kwon

et al. 2017

Sci Rep

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Peroxiredoxins (PRDXs) are important antioxidant enzymes reported to have a role in sperm function and male fertility. However, how PRDXs affects male fertility remain fundamental unanswered questions. We therefore sought to investigate the role of these enzymes in sperm function and fertilisation. In this in vitro trial, mouse spermatozoa were incubated with different concentrations of conoidin A (1, 10, or 100 µM), a specific inhibitor of PRDXs. Our results demonstrated that inhibition of PRDXs by conoidin A significantly decreased the oxidized form of peroxiredoxins (PRDXs-SO3) in spermatozoa. Decreased PRDX activity was associated with a significant reduction in sperm motility parameters, viability, and intracellular ATP, whereas ROS levels, DNA fragmentation, and loss of mitochondrial membrane potential were increased. Simultaneously capacitation and the acrosome reaction were also significantly inhibited perhaps as a consequence of decreased tyrosine phosphorylation and protein kinase-A activity. In addition, fertilisation and early embryonic development were adversely affected following PRDXs inhibition in spermatozoa. Taken together, our data demonstrate that decreased PRDX activity directly affects male fertility due to negative effects on important functions and biochemical properties of spermatozoa, ultimately leading to poor fertilisation and embryonic development.

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“…PRDXs are among the most extensively studied antioxidant enzymes that counter reactive oxygen species by reducing hydrogen peroxide (2,(14)(15)(16)(17)(39)(40)(41). It has been reported that Prdx 4 is associated with sperm acrosome formation and damage to sperm DNA (42).…”

Section: Discussionmentioning

confidence: 99%

Peroxiredoxin 4 as potential fertility marker in boars

Ryu

Pang

Rahman

et al. 2020

Preprint

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Background: High levels of reactive oxygen species are toxic to spermatozoa as they induce DNA damage, which leads to male infertility. Therefore, reduction of oxidative stress using reducing agents, such as antioxidant enzymes, is required. Peroxiredoxins (Prdxs) are known to play a critical role in the regulation of male fertility as antioxidant enzymes. Although several studies have suggested a close association between Prdxs and male fertility, few studies have explored the efficacy of Prdxs to predict fertility. Therefore, the current study was designed to discover the most sensitive biomarkers among the Prdxs with six isoforms, and furthermore to determine whether Prdxs are more suitable fertility markers for boar spermatozoa compared to conventional semen analysis.Results: The mRNA levels of PRDXs and several sperm parameters were examined in spermatozoa collected from 20 boars with different litter sizes. Our study showed that there was a significant positive correlation between the litter size and the levels of PRDX 4 among all isoforms in spermatozoa. Subsequently, a regression analysis using a combination of markers was conducted to increase efficacy for fertility prediction. According to the data, PRDX4 had the highest efficacy compared to other combination models. The prediction accuracy of male fertility was further evaluated through receiver operating characteristic curve analysis, which showed that PRDX 4 could predict the litter size with a high overall accuracy of 95%.Conclusions: As fertility biomarkers, genomic markers might be more accurate for predicting male fertility compared to conventional semen analysis. Our findings indicate that PRDX 4 might be a potential biomarker for diagnosing male fertility and subsequently improving reproductive efficacy in boars.

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Overexpression of Peroxiredoxin 6 Protects Neoplastic Cells against Apoptosis in Canine Haemangiosarcoma

Cited by 15 publications

References 34 publications

Sequenced Combinations of Cisplatin and Selected Phytochemicals towards Overcoming Drug Resistance in Ovarian Tumour Models

Sequenced Combinations of Cisplatin and Selected Phytochemicals towards Overcoming Drug Resistance in Ovarian Tumour Models

Peroxiredoxin activity is a major landmark of male fertility

Peroxiredoxin 4 as potential fertility marker in boars

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