Overexpression of PkINO1 improves ethanol resistance of Pichia kudriavzevii N77-4 isolated from the Korean traditional fermentation starter nuruk

Sugiyama, Minetaka; Baek, Seong Yeol; Takashima, Shohei; Miyashita, Natsumi; Ishida, K; Mun, Ji-Young; Yeo, Soo‐Hwan

doi:10.1016/j.jbiosc.2018.06.001

Cited by 8 publications

(5 citation statements)

References 24 publications

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“…2, 3 and Table 2). Several publications have reported that some P. kudriavzevii strains possess multi-stress tolerance [19,30,31]. These include P. kudriavzevii N77-4 which could tolerate 100 mM acetic acid, but was sensitive to 6% ethanol at 30 °C [31]; P. kudriavzevii RZ8-1 that could grow on medium containing 5 g/l acetic acid (approximately 82.5 mM) or higher than 12% ethanol at 35 °C [19], and soil-isolated P. kudriavzevii that could grow at 12% ethanol, 1.44 g/l (approximately 15 mM) furfural and 2.5 g/l (approximately 41 mM) acetic acid [32].…”

Section: Discussionmentioning

confidence: 99%

“…Several publications have reported that some P. kudriavzevii strains possess multi-stress tolerance [19,30,31]. These include P. kudriavzevii N77-4 which could tolerate 100 mM acetic acid, but was sensitive to 6% ethanol at 30 °C [31]; P. kudriavzevii RZ8-1 that could grow on medium containing 5 g/l acetic acid (approximately 82.5 mM) or higher than 12% ethanol at 35 °C [19], and soil-isolated P. kudriavzevii that could grow at 12% ethanol, 1.44 g/l (approximately 15 mM) furfural and 2.5 g/l (approximately 41 mM) acetic acid [32]. Compared to these reported strains, P. kudriavzevii LC375240 clearly showed the highest tolerance to the inhibitory substances at 42 °C, demonstrating that P. kudriavzevii LC375240 is a better thermotolerant and multi-stress-tolerant strain for bioethanol production.…”

Section: Discussionmentioning

confidence: 99%

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Effects of various inhibitory substances and immobilization on ethanol production efficiency of a thermotolerant Pichia kudriavzevii

Ndubuisi

Qin

Liao

et al. 2020

Biotechnol Biofuels

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Background Although bioethanol production has been gaining worldwide attention as an alternative to fossil fuel, ethanol productivities and yields are still limited due to the susceptibility of fermentation microorganisms to various stress and inhibitory substances. There is therefore an unmet need to search for multi-stress-tolerant organisms to improve ethanol productivity and reduce production cost, particularly when lignocellulosic hydrolysates are used as the feedstock. Results Here, we have characterized a previously isolated Pichia kudriavzevii LC375240 strain which is thermotolerant to high temperatures of 37 °C and 42 °C. More excitingly, growth and ethanol productivity of this strain exhibit strong tolerance to multiple stresses such as acetic acid, furfural, formic acid, H2O2 and high concentration of ethanol at 42 °C. In addition, simple immobilization of LC375240 on corncobs resulted to a more stable and higher efficient ethanol production for successive four cycles of repeated batch fermentation at 42 °C. Conclusion The feature of being thermotolerant and multi-stress-tolerant is unique to P. kudriavzevii LC375240 and makes it a good candidate for second-generation bioethanol fermentation as well as for investigating the molecular basis underlying the robust stress tolerance. Immobilization of P. kudriavzevii LC375240 on corncobs is another option for cheap and high ethanol productivity.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effects of various inhibitory substances and immobilization on ethanol production efficiency of a thermotolerant Pichia kudriavzevii

Ndubuisi

Qin

Liao

et al. 2020

Biotechnol Biofuels

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“…1 a-c). P. kudriavzevii has been shown to tolerate high concentrations of acetic acid: 70 mM [ 47 ], 5 g/l (~ 83.3 mM) [ 41 ], and 100 mM [ 48 ], as well as formic acid at 30 mM [ 49 ]. Our findings suggest that dung beetle-associated yeasts are capable of withstanding exposure to inhibitory organic acids, which is crucial for their application in industrial fermentations.…”

Section: Resultsmentioning

confidence: 99%

“…Group D, consisting of isolates of P. kudriavzevii and P. cecembensis , performed well under all stress conditions except 44 °C and 7 mM H 2 O 2 . Several studies have reported that some P. kudriavzevii strains possess the ability to withstand multiple stresses [ 3 , 41 , 48 , 49 ]. The control yeast in the same group grew moderately well.…”

Section: Resultsmentioning

confidence: 99%

Dung beetle-associated yeasts display multiple stress tolerance: a desirable trait of potential industrial strains

Nwaefuna,

Garcia-Aloy,

Loeto

et al. 2023

BMC Microbiol

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Background Stress-tolerant yeasts are highly desirable for cost-effective bioprocessing. Several strategies have been documented to develop robust yeasts, such as genetic and metabolic engineering, artificial selection, and natural selection strategies, among others. However, the significant drawbacks of such techniques have motivated the exploration of naturally occurring stress-tolerant yeasts. We previously explored the biodiversity of non-conventional dung beetle-associated yeasts from extremophilic and pristine environments in Botswana (Nwaefuna AE et.al., Yeast, 2023). Here, we assessed their tolerance to industrially relevant stressors individually, such as elevated concentrations of osmolytes, organic acids, ethanol, and oxidizing agents, as well as elevated temperatures. Results Our findings suggest that these dung beetle-associated yeasts tolerate various stresses comparable to those of the robust bioethanol yeast strain, Saccharomyces cerevisiae (Ethanol Red™). Fifty-six percent of the yeast isolates were tolerant of temperatures up to 42 °C, 12.4% of them could tolerate ethanol concentrations up to 9% (v/v), 43.2% of them were tolerant to formic acid concentrations up to 20 mM, 22.7% were tolerant to acetic acid concentrations up to 45 mM, 34.0% of them could tolerate hydrogen peroxide up to 7 mM, and 44.3% of the yeasts could tolerate osmotic stress up to 1.5 M. Conclusion The ability to tolerate multiple stresses is a desirable trait in the selection of novel production strains for diverse biotechnological applications, such as bioethanol production. Our study shows that the exploration of natural diversity in the search for stress-tolerant yeasts is an appealing approach for the development of robust yeasts.

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“…Pichia kudriavzevii , synonymously known as Issatchenkia orientalis or Candida krusei , has been isolated from a variety of fermented foods and fruit sources . P .…”

Section: Introductionmentioning

confidence: 99%

Improved cadmium resistance and removal capacity in Pichia kudriavzevii A16 by sucrose preincubation

Zhang

Guo

et al. 2019

J Basic Microbiol

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Removal of heavy metals from food material by growing micro-organisms is limited by the toxicity to cells. In this study, different preincubation treatments were investigated to analyze their effects on cadmium resistance and removal ability of Pichia kudriavzevii A16 and Saccharomyces cerevisiae CICC1211. Sucrose preincubation improved the cadmium resistance of both yeast cells and increased the cadmium-removal rate of P. kudriavzevii A16. An evident decrease of intracellular and cell-surface cadmium accumulation was observed after sucrose preincubation, which may be the primary reason responsible for the improved cadmium resistance. Flow cytometry assay showed that sucrose significantly reduced the production of reactive oxygen species (ROS) and cell death rate of both yeasts under cadmium compared with those normally cultured cells. Under cadmium stress, the content of both protein carbonyls and malonyldialdehyde were also reduced by the addition of sucrose, the results were in accordance with the tendency of ROS, exhibiting a defending function of sucrose. Osmotic regulators as proline and trehalose were increased by sucrose preincubation in P. kudriavzevii A16 in the presence of cadmium. The results suggested that sucrose preincubation could be applied to improve cadmium resistance and removal rate of yeasts. K E Y W O R D Scadmium removal, cadmium resistance, cross-protection, Pichia kudriavzevii, sucrose preincubation

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Overexpression of PkINO1 improves ethanol resistance of Pichia kudriavzevii N77-4 isolated from the Korean traditional fermentation starter nuruk

Cited by 8 publications

References 24 publications

Effects of various inhibitory substances and immobilization on ethanol production efficiency of a thermotolerant Pichia kudriavzevii

Effects of various inhibitory substances and immobilization on ethanol production efficiency of a thermotolerant Pichia kudriavzevii

Dung beetle-associated yeasts display multiple stress tolerance: a desirable trait of potential industrial strains

Improved cadmium resistance and removal capacity in Pichia kudriavzevii A16 by sucrose preincubation

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