Overexpression of programmed cell death 5 in a mouse model of ovalbumin-induced allergic asthma

Abstract

BackgroundProgrammed cell death 5 (PDCD5) was first identified as an apoptosis-promoting protein and involved in some autoimmune diseases and inflammatory processes. Our previous study demonstrated greater expression of serum PDCD5 in asthmatic patients than controls. This study aimed to further explore the significance of PDCD5 in mice with induced allergic asthma.MethodsWe divided 16 female mice into 2 groups: control (n = 8) and allergen (ovalbumin, OVA)-challenged mice (n = 8). The modified ovalbumin inhalation method was used to generate the allergic asthma mouse model, and the impact of OVA was assessed by histology of lung tissue and morphometry. The number of cells in bronchoalveolar lavage fluid (BALF) was detected. Pulmonary function was measured by pressure sensors. PDCD5 and active caspase-3 levels were detected.ResultsThe expression of PDCD5 was higher with OVA challenge than for controls (p < 0.05). PDCD5 level was correlated with number of inflammatory cells in BALF and lung function. Moreover, active caspase-3 level was increased in the OVA-challenged mice (p < 0.001) and correlated with PDCD5 level (p = 0.000).ConclusionsThese data demonstrate an association between level of PDCD5 and asthma severity and indicate that PDCD5 may play a role in allergic asthma.Electronic supplementary materialThe online version of this article (doi:10.1186/s12890-016-0317-y) contains supplementary material, which is available to authorized users.

Network Pharmacology Strategy to Investigate the Pharmacological Mechanism of HuangQiXiXin Decoction on Cough Variant Asthma and Evidence‐Based Medicine Approach Validation

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The Molecular Evolution and Functional Divergence of Lamprey Programmed Cell Death Genes

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Effect of Tiotropium Bromide on Airway Inflammation and Programmed Cell Death 5 in a Mouse Model of Ovalbumin-Induced Allergic Asthma

“…The modified OVA inhalation method was used to generate the allergic asthma mouse model as described in [14]. In brief, OVA sensitization involved an intraperitoneal injection of 20 μ g OVA absorbed with 2.25 mg aluminum hydroxide gel on days 1 and 14.…”

“…Fixed lung tissues were embedded in paraffin, and sections were stained with hematoxylin and eosin (H&E) for inflammatory cell infiltration and proliferation of smooth muscle, Masson's trichrome for airway fibrosis and collagen deposition, and periodic acid-Schiff (PAS) for goblet cells. An expert respiratory pathologist blinded to treatment groups graded the extent of inflammation in the lungs according to a semiquantitative scoring system [14].…”

“…We previously reported increased serum PDCD5 level in asthmatic patients. In addition, we found PDCD5 upregulated in bronchoalveolar lavage fluid (BALF) and lung tissue of ovalbumin- (OVA-) challenged asthmatic mice as compared with controls [13, 14]. Finally, the expression of PDCD5 was correlated with asthma severity and active caspase-3 levels.…”