Overexpression of protein kinase B/AKT induces phosphorylation of p70S6K and 4E-BP1 in goat fetal fibroblasts

Ma, Kai; Nyamtsengel, V.; Bao, Wenlei; Lian, Min; Wang, W.P.; Wang, Y.F.; Wang, X.; Wang, Z.G.

doi:10.4238/2014.november.27.22

Cited by 3 publications

(3 citation statements)

References 31 publications

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“…AKT phosphorylates downstream substrates to regulate cell growth, proliferation, apoptosis, senescence, and other processes [40]. Cong Fu et al found that P-AKT expression was down-regulated during the process of cellular senescence induced by H 2 O 2 [41].…”

Section: Discussionmentioning

confidence: 99%

Soluble Egg Antigens of Schistosoma japonicum Induce Senescence of Activated Hepatic Stellate Cells by Activation of the FoxO3a/SKP2/P27 Pathway

et al. 2016

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BackgroundLiver fibrosis was viewed as a reversible process. The activation of hepatic stellate cells (HSCs) is a key event in the process of liver fibrosis. The induction of senescence of HSCs would accelerate the clearance of the activated HSCs. Previously, we demonstrated that soluble egg antigens (SEA) of Schistosoma japonicum promoted the senescence of HSCs via STAT3/P53/P21 pathway. In this paper, our study was aimed to explore whether there are other signaling pathways in the process of SEA-induced HSCs aging and the underlying effect of SKP2/P27 signal on senescent HSCs.Methodology/Principal findingsHuman hepatic stellate cell line, LX-2 cells, were cultured and stimulated with SEA. Western blot and cellular immunofluorescence analysis were performed to determine the expression of senescence-associated protein, such as P27, SKP2 and FoxO3a. Besides, RNA interfering was applied to knockdown the expression of related protein. The senescence of HSCs was determined by senescence-associated β-gal staining. We found that SEA increased the expression of P27 protein, whereas it inhibited the expression of SKP2 and FoxO3a. Knockdown of P27 as well as overexpression of SKP2 both suppressed the SEA-induced senescence of HSCs. In addition, the nuclear translocation of FoxO3a from the nucleus to the cytoplasm was induced by SEA stimulation.Conclusions/SignificanceThe present study demonstrates that SEA promotes HSCs senescence through the FoxO3a/SKP2/P27 pathway.

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Section: Discussionmentioning

confidence: 99%

Soluble Egg Antigens of Schistosoma japonicum Induce Senescence of Activated Hepatic Stellate Cells by Activation of the FoxO3a/SKP2/P27 Pathway

et al. 2016

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“…One of them, PI3K-AKT-mTOR pathway, is crucial in the coordination of cell survival, differentiation, proliferation, and development during organogenesis [24]. Moreover, PI3K-AKT-mTOR pathway mediates pathophysiological processes in several mammals including goat [25]. In our HFs, this pathway is connected, in addition to pluripotency of stem cells, with estrogen, cell cycle, and Notch signalling pathways, suggesting thath of PI3K-AKT-mTOR pathway is central in the cashmere growth.…”

Section: Discussionmentioning

confidence: 99%

Shedding light on cashmere goat hair follicle biology: from morphology analyses to transcriptomic landascape

et al. 2020

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Background: Cashmere goat is known for its precious undercoat. Being photoperiod-dictated, cashmere growth has been studied focusing mainly on hair follicle cycle phases (anagen, catagen and telogen). An accurate molecular knowledge of the goat hair follicle cycle, disentangling gene expression changes during phases and recognizing timing boundaries, could be useful to improve cashmere goat management and ultimately cashmere production. Results: To better describe goat's hair follicle transcriptome we applied RNA-sequencing to isolated hair follicles from five Italian cashmere goats, during the anagen and catagen phase, identifying total of 214 differentially expressed genes (DEGs): 97 were up-regulated while 117 were down-regulated in catagen with respect to anagen. Gene Ontology and pathway analysis were performed. We detected 144 significant pathways spanning from estrogen, pluripotency of stem cells, thermogenesis and fatty acid metabolism that were strongly expressed during the hair follicle phases analysed. Finally, we validated promising DEGs by RT-qPCR in the same set of samples as well as in hair follicles and entire skin biopsies of another cashmere goats cohort accounting for early anagen, anagen, early catagen, and catagen phases. Conclusions: As in the isolated hair follicles, some target genes were homogenously modulated during the four hair follicle phases. Ceruloplasmin (CP) and Keratin 4 (K4), confirmed their clear cut expression between growing and resting phase. In fact, K4 was almost absent in catagen phases while CP was barely expressed in anagen phases. In particular, the strong expression of K4 in early anagen makes it an eligible marker to track the beginning of a new hair cycle, and therefore defining the optimum time for cashmere harvesting.

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“…Ferroptosis is a non-apoptotic type of regulated and controlled cell death that is biochemically and genetically disparate from other major cell deaths, such as apoptosis, autophagy and necrosis (Dixon 2017 ; Mou et al. 2019 ; Xu et al. 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Red ginseng polysaccharide exhibits anticancer activity through GPX4 downregulation-induced ferroptosis

Zhai

Liang

et al. 2022

Pharmaceutical Biology

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Context Red ginseng polysaccharide (RGP) is an active component of the widely used medicinal plant Panax ginseng C. A. Meyer (Araliaceae), which has displayed promising activities against cancer cells. However, the detailed molecular mechanism of RGP in ferroptosis is still unknown. Objective This study evaluates the effects of RGP in cancer cells. Materials and methods A549 and MDA-MB-231 cells were used. Cell proliferation was measured by CCK-8 assay after being treated with RGP at concentrations of 0, 50, 100, 200, 400, 800 and 1600 μg/mL at 0, 12, 24 and 48 h. Lipid reactive oxygen species (ROS) levels were assessed by C11-BODIPY assay. The control group was treated with PBS. Results RGP inhibited human A549 (IC 50 : 376.2 μg/mL) or MDA-MB-231(IC 50 : 311.3 μg/mL) proliferation and induced lactate dehydrogenase (LDH) release, promoted ferroptosis and suppressed the expression of GPX4. Moreover, the effects of RGP were enhanced by the ferroptosis inducer erastin, while abolished by ferroptosis inhibitor ferrostatin-1. Discussion and conclusions Our study is the first to demonstrate (1) the anticancer activity of RGP in human lung cancer and breast cancer. (2) RGP presented the anti-ferroptosis effects in lung and breast cancer cells via targeting GPX4.

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Overexpression of protein kinase B/AKT induces phosphorylation of p70S6K and 4E-BP1 in goat fetal fibroblasts

Cited by 3 publications

References 31 publications

Soluble Egg Antigens of Schistosoma japonicum Induce Senescence of Activated Hepatic Stellate Cells by Activation of the FoxO3a/SKP2/P27 Pathway

Soluble Egg Antigens of Schistosoma japonicum Induce Senescence of Activated Hepatic Stellate Cells by Activation of the FoxO3a/SKP2/P27 Pathway

Shedding light on cashmere goat hair follicle biology: from morphology analyses to transcriptomic landascape

Red ginseng polysaccharide exhibits anticancer activity through GPX4 downregulation-induced ferroptosis

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