Overexpression of RNF2 Is an Independent Predictor of Outcome in Patients with Urothelial Carcinoma of the Bladder Undergoing Radical Cystectomy

Li, Xiangzhong; Chen, Si Liang; Dong, Pei; Chen, Jie Wei; Wang, Feng Wei; Guo, Sheng; Jiang, Li; Zhou, Fang; Xie, Dan; Liu, Zhuo Wei

doi:10.1038/srep20894

Cited by 17 publications

(18 citation statements)

References 27 publications

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“…23 Li et al found that overexpression of RNF2 was an independent predictor of outcome in patients with urothelial carcinoma of the bladder undergoing radical cystectomy. 24 Chen et al suggested that miR-139-5p overexpression combined with inactivation of the MAPK signaling pathway can reverse the cisplatin resistance of ovarian cancer by suppressing RNF2. 25 In the present study, we found that RNF2 expression was significantly increased in GC tissues and positively correlated with TNM stage and poor prognosis.…”

Section: Discussionmentioning

confidence: 99%

Long non-coding RNA LINC00665 gastric cancer tumorigenesis by regulation miR-149-3p/RNF2 axis

Qi¹,

Xiao²,

Yun-xi³

2019

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Background: Recently, LINC00665 has been reported to be a pivotal regulator in kinds of malignancy, such as lung cancer and liver cancer. However, the functions and underlying mechanisms of LINC00665 in gastric cancer (GC) remain unclear. Materials and methods: We recruited 49 paired GC tissue to explore LINC00665 expression by qRT-PCR. In vitro function assays were used to explore the roles of LINC00665 in GC progression. Moreover, the interaction among LINC00665, miR-149-3p and RNF2 was explored by bioinformatics analysis and luciferase reporter assay. Results: In the present study, we found that LINC00665 expression was significantly elevated in GC tissues and cell lines. High LINC00665 expression was associated with TNM stage, histological grade, and poor prognosis of GC patients. Function assays showed that LINC00665 suppression significantly reduced GC cells viability and invasion ability in vitro. Mechanistic analysis showed that LINC00665 might serve as a ceRNA for miR-149-3p to regulate the expression of RNF2. Conclusion: Our current study revealed the LINC00665/miR-149-3p/RNF2 axis was involved in GC progression, providing novel insights into the treatment for GC.

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Section: Discussionmentioning

confidence: 99%

Long non-coding RNA LINC00665 gastric cancer tumorigenesis by regulation miR-149-3p/RNF2 axis

Qi¹,

Xiao²,

Yun-xi³

2019

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“…RNF2 was reported to act as an oncogene in gastric cancer, 23 lung squamous cell carcinoma, 24 mammary carcinoma, 25 and urothelial carcinoma of the bladder. 26 Li et al reported that over-expression of RNF2 played a crucial role in the prognosis of urothelial carcinoma of the bladder. 26 In the present study, we found that circRNA_100146 sponged miR-149-5p and promoted RNF2 expression, thus accelerating cell proliferation and invasion of bladder cancer cells.…”

Section: Discussionmentioning

confidence: 99%

“… 26 Li et al reported that over-expression of RNF2 played a crucial role in the prognosis of urothelial carcinoma of the bladder. 26 In the present study, we found that circRNA_100146 sponged miR-149-5p and promoted RNF2 expression, thus accelerating cell proliferation and invasion of bladder cancer cells. As revealed by the clinical data analysis, RNF2 was over-expressed in bladder cancer and showed a positive correlation with circRNA_100146 expression.…”

Section: Discussionmentioning

confidence: 99%

Hsa_circRNA_100146 Acts as a Sponge of miR-149-5p in Promoting Bladder Cancer Progression via Regulating RNF2

Wang¹,

Niu²,

Mao³

et al. 2020

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Background Mounting evidence has demonstrated that circular RNAs (circRNAs) play indispensable roles in the progression of bladder cancer. Public database mining showed that hsa_circRNA_100146 (circRNA_100146) was highly expressed in bladder cancer. This study aimed to characterize the biological role of circRNA_100146 and clarify the underlying mechanism in bladder cancer. Methods We evaluated the relationship between circRNA_100146 expression and clinicopathological features. Furthermore, gain- and loss-of-function studies were conducted in bladder cancer cells via transfection with gene-carrying plasmids (over-expression) or specific short hairpin RNAs (knockdown). Moreover, computational algorithms and dual-luciferase reporter assays were performed to explore the possible mechanisms of action. Additionally, in vivo xenograft experiments were performed to further analyze the effect of circRNA_100146 on tumor growth. Results Our data showed that circRNA_100146 expression was increased in bladder cancer tissues and cell lines, and that high expression of circRNA_100146 was correlated with poor patient prognosis. Upregulation of circRNA_100146 promoted cell proliferation, migration, and invasion, and inhibited cell apoptosis, whereas knockdown of circRNA_100146 displayed opposite effects on bladder cancer cells. Notably, circRNA_100146 could combine with miR-149-5p and promote ring finger protein 2 ( RNF2 ) expression, thereby facilitating the progression of bladder cancer. Furthermore, overexpression of RNF2 reversed the effects of circRNA_100146 knockdown on the biological behaviors of bladder cancer cells. The in vivo experiments revealed that downregulation of circRNA_100146 dramatically delayed tumor growth. Conclusion Our findings indicate that circRNA_100146 functions as a sponge of miR-149-5p in promoting bladder cancer progression by regulating RNF2 expression and that circRNA_100146 may serve as a novel biomarker in human bladder cancer.

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“…Interestingly several studies published up to now described worsening of prognoses with increased RNF2 expression in cancers of pancreas [ 14 ], esophagus [ 15 ], liver [ 16 ], urinary bladder [ 17 ] and ovary [ 18 ]. It is possible that spectrum of genes actively modulated by RNF2 changes in different cell types [ 10 , 19 , 20 ].…”

Section: Discussionmentioning

confidence: 99%

Enhanced intratumoral expression of RNF2 is a favorable prognostic factor for patients with cutaneous melanoma?

et al. 2018

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Recent studies involving melanoma cell lines suggest that enhanced expression of epigenetic regulator RNF2 supports proliferation and promotes metastasis. However, it is not clear to what extent those data apply to disease progression and prognosis for melanoma patients. Therefore the aim of the present study was to assess the prognostic power of RNF2 intratumoral expression by melanoma cells.RNF2 was detected immunohistochemically in standard formalin-fixed paraffin-embedded samples of 9 benign nevi, 60 melanomas and 24 nodal metastases.The lowest percentage of RNF2-positive melanocytes found in nevi was comparable to expression levels in normal skin. The RNF2 expression found in melanomas was significantly higher and it was even more enhanced in metastases. The increased occurrence of RNF2 expressing cells was positively correlated with longer patients’ overall survival. Moreover, a negative correlation was found between intratumoral RNF2 expression and number of generated metastatic lesions.Our data indicate that development of melanoma is associated with significant changes in RNF2 intratumoral expression and imply that at least for some patients the enhancement of the expression levels of RNF2 in both primary and metastatic lesions may be considered a favorable prognostic factor in melanoma.

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Overexpression of RNF2 Is an Independent Predictor of Outcome in Patients with Urothelial Carcinoma of the Bladder Undergoing Radical Cystectomy

Cited by 17 publications

References 27 publications

<p>Long non-coding RNA LINC00665 gastric cancer tumorigenesis by regulation miR-149-3p/RNF2 axis</p>

<p>Long non-coding RNA LINC00665 gastric cancer tumorigenesis by regulation miR-149-3p/RNF2 axis</p>

<p>Hsa_circRNA_100146 Acts as a Sponge of miR-149-5p in Promoting Bladder Cancer Progression via Regulating <em>RNF2</em></p>

Enhanced intratumoral expression of RNF2 is a favorable prognostic factor for patients with cutaneous melanoma?

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