Overexpression of SASH1 Inhibits the Proliferation, Invasion, and EMT in Hepatocarcinoma Cells

He, Ping; Zhang, Hongxia; Sun, Cuiyun; Chen, Chun-yong; Jiang, He-qing

doi:10.3727/096504016x14575597858609

Cited by 27 publications

(17 citation statements)

References 17 publications

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“…SASH1 encodes a scaffold protein involved in the toll-like receptor 4, TLR4 signaling pathway that regulates the cascade of cytokine production and endothelial cell migration in response to invading pathogens [39]. SASH1 is shown to be downregulated in breast cancer [40] and, when highly expressed, SASH1 can inhibit proliferation, invasion, and endothelial mesenchymal transition in other malignancies such as hepatocellular carcinoma and gastric cancer [41,42]. ZDHHC8P1 is upregulated and differentially expressed in two preeclamptic patient cohorts.…”

Section: Discussionmentioning

confidence: 99%

A Review of Candidate Genes and Pathways in Preeclampsia–An Integrated Bioinformatical Analysis

Mohamad

Manzor

Zulkifli

et al. 2020

Biology

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Preeclampsia is a pregnancy-specific disorder characterized by the presence of hypertension with the onset of either proteinuria, maternal organ or uteroplacental dysfunction. Preeclampsia is one of the leading causes of maternal and fetal mortality and morbidity worldwide. However, the etiopathologies of preeclampsia are not fully understood. Many studies have indicated that genes are differentially expressed between normal and in the disease state. Hence, this study systematically searched the literature on human gene expression that was differentially expressed in preeclampsia. An electronic search was performed through 2019 through PubMed, Scopus, Ovid-Medline, and Gene Expression Omnibus where the following MeSH (Medical Subject Heading) terms were used and they had been specified as the primary focus of the articles: Gene, placenta, preeclampsia, and pregnancy in the title or abstract. We also found additional MeSH terms through Cochrane Library: Transcript, sequencing, and profiling. From 687 studies retrieved from the search, only original publications that had performed high throughput sequencing of human placental tissues that reported on differentially expressed genes in pregnancies with preeclampsia were included. Two reviewers independently scrutinized the titles and abstracts before examining the eligibility of studies that met the inclusion criteria. For each study, study design, sample size, sampling type, and method for gene analysis and gene were identified. The genes listed were further analyzed with the DAVID, STRING and Cytoscape MCODE. Three original research articles involving preeclampsia comprising the datasets in gene expression were included. By combining three studies together, 250 differentially expressed genes were produced at a significance setting of p < 0.05. We identified candidate genes: LEP, NRIP1, SASH1, and ZADHHC8P1. Through GO analysis, we found extracellular matrix organization as the highly significant enriched ontology in a group of upregulated genes and immune process in downregulated genes. Studies on a genetic level have the potential to provide new insights into the regulation and to widen the basis for identification of changes in the mechanism of preeclampsia. Integrated bioinformatics could identify differentially expressed genes which could be candidate genes and potential pathways in preeclampsia that may improve our understanding of the cause and underlying molecular mechanisms that could be used as potential biomarkers for risk stratification and treatment.

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Section: Discussionmentioning

confidence: 99%

A Review of Candidate Genes and Pathways in Preeclampsia–An Integrated Bioinformatical Analysis

Mohamad

Manzor

Zulkifli

et al. 2020

Biology

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“…Several studies report that SASH1 is down‐regulated in tumours and that this expression is correlated with tumour grade and prognosis. SASH1 is down‐regulated in a number of cancers, including HCC, breast cancer, gastric cancer, etc . Consistently, in this study, SASH1 was underexpressed in the ESCC cell lines, suggesting that SASH1 might be a candidate tumour suppressor gene in ESCC.…”

Section: Discussionmentioning

confidence: 99%

“…The encoded protein has also been described as a potential tumour suppressor that negatively regulates the proliferation and aggressiveness of multiple cancer cells. He et al reported that the overexpression of SASH1 inhibits proliferation, invasion and epithelial‐mesenchymal transition (EMT) in hepatocarcinoma cells . SASH1 overexpression in lung cancer cells also inhibits the migration/invasion and the protein expression of cyclin D1, matrix metalloproteinase‐1 (MMP‐1), and MMP‐2 .…”

Section: Introductionmentioning

confidence: 99%

MiR‐130b promotes the progression of oesophageal squamous cell carcinoma by targeting SASH1

Zhu

Peng

et al. 2018

J Cellular Molecular Medi

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MiR‐130b and SAM and SH3 domain containing 1 (SASH1) play an important role in many types of human cancers. The aim of our research was to study their interactions in the process of the proliferation and aggressiveness of oesophageal squamous cell carcinoma (ESCC) cells. Microarray analysis was done to screen the differentially expressed genes in the ESCC tissues. miR‐130b and SASH1 mRNA levels in the ESCC tissues and cells were detected by qRT‐PCR. Dual luciferase reporter system was used to verify the target relationship between miR‐130b and SASH1. The effects of miR‐130b on SASH1 expression were explored by western blot in KYSE30 and TE1 cell lines. CCK‐8 assay, flow cytometry, Transwell, and wound healing assays were conducted to explore the effects of miR‐130b and SASH1 in vitro. In addition, in vivo experiments were conducted to study the roles of miR‐130b and SASH1. miR‐130b was highly expressed, while SASH1 was the opposite in both the ESCC tissues and cells. The expression of SASH1 was inhibited by the direct binding of miR‐130b. The inhibition of miR‐130b reduced the proliferation and aggressiveness of ESCC cells, while it also induced apoptosis and cell cycle arrest in the ESCC cells by suppressing SASH1. The in vivo assay suggested that the overexpression of miR‐130b promoted the growth of ESCC tumours. MiR‐130b was up‐regulated in the ESCC tumour tissues and cells, acting as a tumour promoter. A stimulating effect was demonstrated on ESCC cell growth and aggressiveness by suppressing SASH1, which is an anti‐oncogene.

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“…Some studies indicate that SASH1 downregulation is associated with tumor metastasis [3,5]. Other studies indicate downregulated SASH1 promotes metastasis of hepatoma carcinoma cells through Shh signal pathway [7].…”

Section: Introductionmentioning

confidence: 99%

A Novel SASH1-IQGAP1-E-Cadherin Signal Cascade Mediates Breast Cancer Metastasis

Zhou¹,

Zeng²,

Li³

et al. 2020

Breast Cancer Biology

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SAM and SH3 domain-containing protein 1 (SASH1) was previously described as a candidate tumor suppressor gene in breast cancer and colon cancer to mediate tumor metastasis and tumor growth. However, the underlying mechanism that SASH1 implements breast cancer metastasis in most solid cancers remains unexplored. In this study, SASH1 was identified to bind to IQ motif-containing GTPase activating protein 1 (IQGAP1). In breast cancer tissues, there was a correlation between the expressions of SASH1 and IQGAP1 (P < 0.05), and the expressions of SASH1 and IQGAP1 proteins were, respectively, correlated with the expression of E-cadherin (P < 0.001). In addition, the expressions of SASH1 and IQGAP1 proteins were correlated with tumor diameter and tumor grade (all P < 0.05) but without lymph node metastasis (P > 0.05). Therefore, it is suggested that SASH1 may form a new signaling cascade with IQGAP1 and E-cadherin to regulate breast cancer metastasis.

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Overexpression of SASH1 Inhibits the Proliferation, Invasion, and EMT in Hepatocarcinoma Cells

Cited by 27 publications

References 17 publications

A Review of Candidate Genes and Pathways in Preeclampsia–An Integrated Bioinformatical Analysis

A Review of Candidate Genes and Pathways in Preeclampsia–An Integrated Bioinformatical Analysis

MiR‐130b promotes the progression of oesophageal squamous cell carcinoma by targeting SASH1

A Novel SASH1-IQGAP1-E-Cadherin Signal Cascade Mediates Breast Cancer Metastasis

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