Overexpression of SNHG12 regulates the viability and invasion of renal cell carcinoma cells through modulation of HIF1α

Chen, Qiguang; Zhou, Wei; Du, Shanshan; Gong, Daxin; Li, Jun; Bi, Jianbin; Li, Zhenhua; Zhang, Zhe; Li, Zeliang; Liu, Xiankui; Kong, Chuize

doi:10.1186/s12935-019-0782-5

Cited by 24 publications

(28 citation statements)

References 37 publications

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“…SNHG12 has various biological functions through targeting of cell proliferation, invasion, migration, and apoptosis. SNHG12 is involved in different types of malignant diseases by targeting the following microRNAs: miR-424-5p in cervical cancer (124), miR-320 in gastric cancer (125), miR-199a-5p in renal cell carcinoma (126), miR-199a/b in hepatocellular carcinoma (32), miR-199a/b in gastric cancer (127), miR-138 in lung cancer (32), miR-181a in lung cancer (128), miR-16-5p in thyroid carcinoma (129), miR-129-5p in laryngeal squamous cell carcinoma (130), miR-199-5p in renal carcinoma (126), and miR-195-5p in osteosarcoma (131).…”

Section: Small Nucleolar Rna Host Gene 12 (Snhg12)mentioning

confidence: 99%

An Emerging Class of Long Non-coding RNA With Oncogenic Role Arises From the snoRNA Host Genes

et al. 2020

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The small nucleolar RNA host genes (SNHGs) are a group of long non-coding RNAs, which are reported in many studies as being overexpressed in various cancers. With very few exceptions, the SNHGs (SNHG1, SNHG3, SNHG5, SNHG6, SNHG7, SNHG12, SNHG15, SNHG16, SNHG20) are recognized as inducing increased proliferation, cell cycle progression, invasion, and metastasis of cancer cells, which makes this class of transcripts a viable biomarker for cancer development and aggressiveness. Through our literature research, we also found that silencing of SNHGs through small interfering RNAs or short hairpin RNAs is very effective in both in vitro and in vivo experiments by lowering the aggressiveness of solid cancers. The knockdown of SNHG as a new cancer therapeutic option should be investigated more in the future.

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Section: Small Nucleolar Rna Host Gene 12 (Snhg12)mentioning

confidence: 99%

An Emerging Class of Long Non-coding RNA With Oncogenic Role Arises From the snoRNA Host Genes

et al. 2020

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“…In prostate cancer, SNHG12 induced the activation of Wnt/β-catenin signaling to accelerate cell proliferation by absorbing microRNA-95 [27]. Likewise, an abundance of SNHG12 promoted viability, migration, and invasion both in ovarian cancer and renal cell carcinoma cells through regulation of miRNA-129/SOX4 axis and HIF1α, respectively [28,29]. Moreover, overexpression of SNHG12 induced by c-Myc facilitated proliferation and weakened cisplatin sensitivity in T-cell lymphoma by regulating Ki67/P-gp [30].…”

Section: Discussionmentioning

confidence: 99%

Upregulation of SNHG12 accelerates cell proliferation, migration, invasion and restrain cell apoptosis in breast cancer by enhancing regulating SALL4 expression via sponging miR-15a-5p

Yuan¹,

Li²,

Hu³

et al. 2020

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Breast cancer (BC) is malignant cancer that threatens the health of millions of females worldwide. Long non-coding RNA (lncRNA) small nucleolar RNA host gene 12 (SNHG12) has been identified as an oncogene in multiple cancers. However, the regulatory role of SNHG12 in BC cell progression is still obscured. The levels of SNHG12, miR-15a-5p, and Sal-like 4 (SALL4) in BC tumor tissues and cells were measured by qRT-PCR. Cell viability, apoptosis, migration, and invasion were examined by CCK8, flow cytometry, and transwell assay, respectively. The interaction between miR-15a-5p and SNHG12 or SALL4 was evaluated by dual-luciferase reporter assay. Protein expression of SALL4 was analyzed by western blot. Xenograft mice were established by subcutaneously injecting BC cells stably transfected with sh-SNHG12 and sh-NC. SNHG12 and SALL4 expressions were upregulated whereas miR-15a-5p was downregulated in BC tumors compared with normal tissues. Besides, miR-15a-5p was correlated with SNHG12 and SALL4 inversely as calculated by Pearson's correlation coefficient. More importantly, SNHG12 knockdown attenuated BC tumor growth in vitro and in vivo. Subsequently, dual-luciferase reporter assay confirmed the interaction between miR-15a-5p and SNHG12 or SALL4. The rescue experiments revealed that miR-15a-5p inhibitor restored SNHG12 silencing induced inhibition on BC cell proliferation, migration, invasion, and promotion of apoptosis. Additionally, SNHG12 was found to accelerate BC cell progression by absorbing miR-15a-5p to enhance SALL4 expression. SNHG12 promotes cell proliferation, migration, and invasion but suppresses apoptosis in BC by upregulating SALL4 expression via sponging miR-15a-5p, representing potential targets for the development of novel diagnosis and treatment methods.

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“…As depicted in Figure 5 (17), ovarian cancer (14), colorectal cancer (42), and osteosarcoma (18). Additionally, upregulated SNHG12 expression levels play important roles in the cellular process of tumorigenesis, including cancer cell proliferation (12,19,39,43), migration (18,37), invasion (36,38), apoptosis (16,44), epithelial-mesenchymal transition (EMT) (17) and chemoresistance (45).…”

Section: Online Cross-validation In Tcga Datasetmentioning

confidence: 99%

“…The present evidence revealed that SNHG12 could function as ceRNA by directly binding to miRNA and thereby regulating target genes in human cancers. For instance, SNHG12 upregulation increased the expression of hypoxia-inducible factor 1 α (HIF1α) by targeting miR-199a-5p, which induced cell proliferation, migration, and invasion in renal cell carcinoma (38). Moreover, doxorubicin resistance in osteosarcoma was promoted by SNHG12 via targeting miR-320a to upregulate myeloid cell leukemia 1 (MCL1) (45).…”

Section: Online Cross-validation In Tcga Datasetmentioning

confidence: 99%

“…Recently, increasing evidence of next-generation genome wide sequencing and single-cell RNA-sequencing has revealed that lncRNAs have aberrant expressions and mutations in human pan-cancers(29)(30)(31). More and more studies have showed that abnormally expressed lncRNAs are emerging as important regulators in tumorigenesis and show a significant association with cancer prognosis(32)(33)(34)(35).SNHG12 is dysregulated in multiple kinds of human cancers, including prostate cancer(12), gastric cancer(19), cervical cancer(36), hepatocellular carcinoma(37), renal cell carcinoma(38), nasopharyngeal carcinoma(39), glioma(40), breast cancer(41), non-small cell lung cancer…”

mentioning

confidence: 99%

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Prognostic and clinical significance of long non-coding RNA SNHG12 expression in various cancers

Zhang

Ren

et al. 2020

Preprint

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Background: Recently, dysregulation of lncRNA SNHG12 has been determined in kinds of cancers. However, definite prognostic value of SNHG12 remains unclear. We conducted this meta-analysis to evaluate the association between SNHG12 expression levels and caner prognosis.Methods: A literature retrieval was conducted by searching kinds of databases. The meta-analysis of prognostic and clinicopathological parameters was performed by using Revman 5.2 and Stata 12.0 software. Besides, The Cancer Genome Atlas dataset was analyzed to validate the results in our meta-analysis via using Gene Expression Profiling Interactive Analysis.Results: High SNHG12 expression significantly predicted worse overall survival (HR=1.97, 95%CI 1.56-2.48, P<0.01) and recurrence-free survival (HR=1.71, 95%CI 1.05-2.78, P<0.01). Tumor type, sample size, survival analysis method, and cut-off value did not alter SNHG12 prognosis value according to stratified analysis results. Additionally, patients with elevated SNHG12 expression were more prone to unfavorable clinicopathological outcomes, including larger tumor size, lymph node metastasis, distant metastasis, advanced clinical stage. Online cross-validation in TCGA dataset further proved that cancer patients with upregulated SNHG12 expression had worse overall survival and disease-free survival.Conclusion: Elevated SNHG12 expression was associated with poor survival and unfavorable clinicopathological features in various cancers, and therefore might be a potential prognostic biomarker in human cancers.

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Overexpression of SNHG12 regulates the viability and invasion of renal cell carcinoma cells through modulation of HIF1α

Cited by 24 publications

References 37 publications

An Emerging Class of Long Non-coding RNA With Oncogenic Role Arises From the snoRNA Host Genes

An Emerging Class of Long Non-coding RNA With Oncogenic Role Arises From the snoRNA Host Genes

Upregulation of SNHG12 accelerates cell proliferation, migration, invasion and restrain cell apoptosis in breast cancer by enhancing regulating SALL4 expression via sponging miR-15a-5p

Prognostic and clinical significance of long non-coding RNA SNHG12 expression in various cancers

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