Overexpression of StRbohA in Arabidopsis thaliana enhances defence responses against Verticillium dahliae

Yao, Zhen; Islam, Md. Rashidul; Badawi, Mohamed; El-Bebany, Ahmed F.; Daayf, Fouad

doi:10.1016/j.pmpp.2015.04.003

Cited by 6 publications

(3 citation statements)

References 62 publications

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“…However, several studies have pointed to the essential role of RBOH-generated ROS also in HR cell death and/or HR-conferred resistance. This includes viral pathosystems [ 15 ] such as potato–potato virus Y interaction [ 16 ] and one of the most studied viral pathosystems, Nicotiana tabacum—tobacco mosaic virus (TMV) [ 17 , 18 ], bacterial [ 19 , 20 , 21 , 22 , 23 ], oomycete [ 19 , 24 , 25 ], and fungal pathosystems [ 23 , 26 , 27 , 28 , 29 ]. The results of the above-mentioned studies show that the role of RBOH-generated ROS in immunity is pathosystem- but also RBOH isoform-dependent [ 14 ].…”

Section: Reactive Oxygen Species As One Of the Earliest Hallmarks Of ...mentioning

confidence: 99%

Intertwined Roles of Reactive Oxygen Species and Salicylic Acid Signaling Are Crucial for the Plant Response to Biotic Stress

Lukan

Coll

2022

IJMS

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One of the earliest hallmarks of plant immune response is production of reactive oxygen species (ROS) in different subcellular compartments, which regulate plant immunity. A suitable equilibrium, which is crucial to prevent ROS overaccumulation leading to oxidative stress, is maintained by salicylic acid (SA), a chief regulator of ROS. However, ROS not only act downstream of SA signaling, but are also proposed to be a central component of a self-amplifying loop that regulates SA signaling as well as the interaction balance between different phytohormones. The exact role of this crosstalk, the position where SA interferes with ROS signaling and ROS interferes with SA signaling and the outcome of this regulation, depend on the origin of ROS but also on the pathosystem. The precise spatiotemporal regulation of organelle-specific ROS and SA levels determine the effectiveness of pathogen arrest and is therefore crucial for a successful immune response. However, the regulatory interplay behind still remains poorly understood, as up until now, the role of organelle-specific ROS and SA in hypersensitive response (HR)-conferred resistance has mostly been studied by altering the level of a single component. In order to address these aspects, a sophisticated combination of research methods for monitoring the spatiotemporal dynamics of key players and transcriptional activity in plants is needed and will most probably consist of biosensors and precision transcriptomics.

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Section: Reactive Oxygen Species As One Of the Earliest Hallmarks Of ...mentioning

confidence: 99%

Intertwined Roles of Reactive Oxygen Species and Salicylic Acid Signaling Are Crucial for the Plant Response to Biotic Stress

Lukan

Coll

2022

IJMS

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“…This study provides a detailed follow up on the temporal expression of genes identified in our previous studies [ 22 , 23 ]. Together with more recent functional analyses of selected genes and interaction with host defense components [ 21 , 24 , 131 ], this study provides another background to reflect on which metabolic reactions contribute to disease, and which ones rather contribute to less disease or lack of it. The work ahead should put emphasis on the spatiotemporal expression of these genes during these interactions in order to fine-tune the expression map of genes that are important for the pathogen to invade its hosts.…”

Section: Discussionmentioning

confidence: 85%

Gene Expression of Putative Pathogenicity-Related Genes in Verticillium dahliae in Response to Elicitation with Potato Extracts and during Infection Using Quantitative Real-Time PCR

et al. 2021

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Quantitative real-time PCR was used to monitor the expression of 15 Verticillium dahliae’s genes, putatively involved in pathogenicity, highly (HAV) and weakly aggressive (WAV) V. dahliae isolates after either (i) elicitation with potato leaf, stem, or root extracts, or (ii) inoculation of potato detached petioles. These genes, i.e., coding for Ras-GAP-like protein, serine/threonine protein kinase, Ubiquitin-conjugating enzyme variant-MMS2, NADH-ubiquinone oxidoreductase, Thioredoxin, Pyruvate dehydrogenase E1 VdPDHB, myo-inositol 2-dehydrogenase, and HAD-superfamily hydrolase, showed differential upregulation in the HAV versus WAV isolate in response to plant extracts or after inoculation of potato leaf petioles. This suggests their potential involvement in the observed differential aggressiveness between isolates. However, other genes like glucan endo-1,3-alpha-glucosidase and nuc-1 negative regulatory protein VdPREG showed higher activity in the WAV than in the HAV in response to potato extracts and/or during infection. This, in contrast, may suggest a role in their lower aggressiveness. These findings, along with future functional analysis of selected genes, will contribute to improving our understanding of V. dahliae’s pathogenesis. For example, expression of VdPREG negatively regulates phosphorus-acquisition enzymes, which may indicate a lower phosphorus acquisition activity in the WAV. Therefore, integrating the knowledge about the activity of both genes enhancing pathogenicity and those restraining it will provide a guild line for further functional characterization of the most critical genes, thus driving new ideas towards better Verticillium wilt management.

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“…However, several studies have pointed to the essential role of RBOH-generated ROS also in HR cell death and/or HR-conferred resistance. This includes viral pathosystems [15], such as potato-potato virus Y interaction [16] and one of the most studied viral pathosystems Nicotiana tabacum -tobacco mosaic virus (TMV) [17,18] bacterial pathosystems [19][20][21][22][23], oomycete pathosystems [19,24,25] and fungal pathosystems [23,[26][27][28][29]. The results of above mentioned studies show that the role of RBOH-generated ROS in immunity is pathosystem-, but also RBOH isoform-dependent [14].…”

mentioning

confidence: 99%

Intertwined Roles of Reactive Oxygen Species and Salicylic Acid Signaling Are Crucial for the Plant Response to Biotic Stress

Lukan¹,

Coll²

2022

Preprint

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One of the earliest hallmarks of plant immune response is production of reactive oxygen spe-cies (ROS) in different subcellular compartments, which regulate plant immunity. A suitable equilibrium, which is crucial to prevent ROS over-accumulation leading to oxidative stress, is maintained by salicylic acid (SA), a chief regulator of ROS. However, ROS are not only acting downstream of SA signaling, but were also proposed to be a central component of a self-amplifying loop that regulates SA signaling as well as the interaction balance between dif-ferent phytohormones. The exact role of this crosstalk, the position where SA interferes with ROS signaling and ROS interferes with SA signaling and the outcome of this regulation depend on the origin of ROS but also on the pathosystem. The precise spatiotemporal regulation of or-ganelle specific ROS and SA levels determine the effectiveness of pathogen arrest and is there-fore crucial for a successful immune response. However, the regulatory interplay behind still remain poorly understood, as up till now, the role of organelle specific ROS and SA in HR-conferred resistance has mostly been studied by altering the level of a single component. In order to address these aspects, a sophisticated combination of research methods for monitoring the spatiotemporal dynamics of key players and transcriptional activity in plants is needed, and will most probably consist of biosensors and precision transcriptomics.

show abstract

Overexpression of StRbohA in Arabidopsis thaliana enhances defence responses against Verticillium dahliae

Cited by 6 publications

References 62 publications

Intertwined Roles of Reactive Oxygen Species and Salicylic Acid Signaling Are Crucial for the Plant Response to Biotic Stress

Intertwined Roles of Reactive Oxygen Species and Salicylic Acid Signaling Are Crucial for the Plant Response to Biotic Stress

Gene Expression of Putative Pathogenicity-Related Genes in Verticillium dahliae in Response to Elicitation with Potato Extracts and during Infection Using Quantitative Real-Time PCR

Intertwined Roles of Reactive Oxygen Species and Salicylic Acid Signaling Are Crucial for the Plant Response to Biotic Stress

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