Overexpression of suadea salsa S-adenosylmethionine synthetase gene promotes salt tolerance in transgenic tobacco

Qi, Yuancheng; Wang, Feifei; Zhang, Hui; Liu, Weiqun

doi:10.1007/s11738-009-0403-3

Cited by 71 publications

(53 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Because SAM is an important factor in primary and secondary metabolism, various effects may be explained via plant transformation with a SAMS gene (Belny et al 1997). SAMS genes under the control of the CaMV 35S constitutive promoter were introduced in Arabidopsis thaliana (Peleman et al 1989a, b), Papaver somniferum (Belny et al 1997), and Nicotiana tabacum (Boerjan et al 1994;Belbahri et al 2000;Qi et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Identification and Functional Analysis of S-AdenosylMethionine Synthetase (HvSAMS) genes in Early Maturing Barley (Hordeum vulgare subsp. vulgare)

Kim¹

2013

Plant Breed. Biotech.

View full text Add to dashboard Cite

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT Grain development has been shown to involve a complex series of physiological and molecular events. In order to provide information on molecular events during grain development, we isolated four noble genes, HvSAMS1, 2, 3 and 4 (Hordeum vulgare S-AdenosylMethionine Synthetase1, 2, 3 and 4). The four HvSAMS genes were closely related based on peptide comparisons and sequence homologies that ranged from 89% to 99%. To determine the developmental expression of HvSAMS genes, Northern blot analysis and RT-PCR were performed with the kernels of -3 to 30 days after fertilization (DAF). The transcripts of HvSAMS1 and 3 genes peaked at -3 DAF that lasted until 7 DAF, and started to reduce at 10 DAF. A strong expression signal of HvSAMS2 was detected from -3 DAF and reached the maximum level at 3 DAF, then decreased until 30 DAF. The expression of HvSAMS4 initially elevated from -3 DAF, reaching the peak at 10 DAF, and decreased gradually until 30 DAF. The HvSAMS gene transcripts were accumulated abundantly in grains, stems and leaves. To evaluate subcellular localization molecular functions of HvSAMS1 gene, we transformed the HvSAMS1 gene into onion epidermal cell and Arabidopsis. Expression of HvSAMS1 recognized by 35S::HvSAMS1::GFP was detected in the nucleus and slightly in the cytosol, whereas 35S::GFP expressed throughout the cell. The transgenic lines showed slightly early germination on MS-medium containing 1 μM GA3 coupled with accelerated extension of bolts. The HvSAMS genes were dominantly expressed in grains during grain development (3 DAF). The HvSAMS genes showed various transcript accumulations in response to the abiotic stresses and exogenous application of phytohormones. Especially, HvSAMS genes were regulated by exogenous GA3. The subcellular localization of HvSAMS1 and histochemical localization of HvSAMS2 promoter provided opportunities to elucidate their possible cellular functions. The phenotypic attributes displayed by HvSAMS1 overexpressing transgenic plants suggested the role of HvSAMS1 in the germination and GA3 response mechanism.

show abstract

Section: Introductionmentioning

confidence: 99%

Identification and Functional Analysis of S-AdenosylMethionine Synthetase (HvSAMS) genes in Early Maturing Barley (Hordeum vulgare subsp. vulgare)

Kim¹

2013

Plant Breed. Biotech.

View full text Add to dashboard Cite

show abstract

“…It had been verified that the overexpression of SAMS could effectively improve the stress tolerances, i.e. cold stress and salt stress of plant (Sodabeh et al, 2013, Qi et al, 2010. Based on the results aforementioned, another role of SAMS was to implicate in the tolerance to drought stress, and AM symbiosis of pigeon pea might up-regulated the expression of CcSAMS, which at least partially lead to the elevated tolerance to drought stress in this species.…”

Section: Figmentioning

confidence: 92%

Molecular cloning of an S-adenosylmethionine synthase gene from pigeon pea (Cajanus cajan) and its expression analysis under arbuscular mycorrhizae fungi colonization and drought stress

Qiao

Zhang

Wen

2017

View full text Add to dashboard Cite

An S-adenosylmethionine synthase (SAMS) gene associated with the drought responsiveness was isolated and characterized from pigeon pea. It was designated CcSAMS and contained an open reading frame of 1,182 bp, which encoded 394 amino acid residues. Sequence analysis of the cloned cDNA showed 94% identity with SAMS from other plant species, suggesting that this gene was considerably conserved in plants. Gene expression analysis demonstrated that CcSAMS was highly expressed in the leaves of AM-colonized plants, irrespective of exposure to either drought or drought-free. Rather, the expression levels of AM plants were significantly higher than that of NAM plants as subjected to drought stress. Therefore, AM symbiosis might enhance the expression of CcSAMS, and the elevated tolerance of AM-colonized pigeon pea to drought-stress was at least partially ascribed to the overexpression of SAMS gene.

show abstract

“…1). Previous study has reported that two SAMS signature motifs, GAGDQGHMFGY and GGGAFSGKD, are conserved among many species (Hilti et al 2000;Qi et al. 2010).…”

Section: Amino Acid Sequence Analysis and Alignmentmentioning

confidence: 99%

“…Functional studies have shown the effects of SAMS on abiotic stress. For example, overexpression of SAMS in alfalfa and tobacco could improve their salinity tolerance (Qi et al 2010;Hua et al 2012). Low temperature conditions can induce the expression of SAMS in O. sativa, A. thaliana, and Zea mays (Cui et al 2005;Yang et al 2006;Uváčková et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Characterization of S-adenosylmethionine Synthetase Gene from Red Algae, Pyropia tenera

Han

Hong

Han

et al. 2016

Plant Breed. Biotech.

View full text Add to dashboard Cite

S-adenosylmethionine (SAM) is widely involved in variety of biosynthetic processes. It participates in the methionine (Met) metabolic pathway. SAM synthetase (EC 2.5.1.6) is the main enzyme that creates SAM from Met and adenosine triphosphate. SAMS genes are highly conserved in different species. We have isolated SAMS genes from red algae, Pyropia tenera. It has been reported that SAMS genes can confer abiotic stress-related response and growth of plant organs in other species. To elucidate the function of PtSAMS gene in evolutionary perspective, we analyzed SAMS gene using bioinformatics tools and plant transformation of Arabidopsis.

show abstract

Overexpression of suadea salsa S-adenosylmethionine synthetase gene promotes salt tolerance in transgenic tobacco

Cited by 71 publications

References 42 publications

Identification and Functional Analysis of S-AdenosylMethionine Synthetase (HvSAMS) genes in Early Maturing Barley (Hordeum vulgare subsp. vulgare)

Identification and Functional Analysis of S-AdenosylMethionine Synthetase (HvSAMS) genes in Early Maturing Barley (Hordeum vulgare subsp. vulgare)

Molecular cloning of an S-adenosylmethionine synthase gene from pigeon pea (Cajanus cajan) and its expression analysis under arbuscular mycorrhizae fungi colonization and drought stress

Characterization of S-adenosylmethionine Synthetase Gene from Red Algae, Pyropia tenera

Contact Info

Product

Resources

About