Overexpression of TaNHX3, a vacuolar Na+/H+ antiporter gene in wheat, enhances salt stress tolerance in tobacco by improving related physiological processes

Lu, Wenjing; Guo, Chengjin; Li, Xiaojuan; Duan, Weiwei; Ma, Chunying; Zhao, Miao; Gu, Jiafeng; Du, Xiaoming; Liu, Zhuling; Xiao, Kai

doi:10.1016/j.plaphy.2013.12.013

Cited by 52 publications

(23 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to the system of nomenclature (Maser et al 2001), NHXs belong to the monovalent cation/proton antiporter-1 (CPA1) family, which generally exhibit 10-12 transmembrane-spanning a-helices and contain a conserved amiloride-binding domain: LFFIYLLPPI (Orlowski and Grinstein 1997). NHXs have been isolated from various plant species, such as Arabidopsis thaliana , rice (Oryza sativa) (Fukuda et al 2011), cotton (Gossypium hirsutum) (Wu et al 2004), wheat (Triticum aestivum) Lu et al 2014), and chrysanthemum (Dendranthema morifolium) (Zhang et al 2012), implying significant roles of this family during evolution. A phylogenetic analysis has revealed that the Arabidopsis NHX family comprises eight members (AtNHX1 to At-NHX8) (Maser et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Identification, functional characterization, and expression pattern of a NaCl-inducible vacuolar Na+/H+ antiporter in chicory (Cichorium intybus L.)

Liang

Lin

et al. 2014

Plant Growth Regul

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Identification, functional characterization, and expression pattern of a NaCl-inducible vacuolar Na+/H+ antiporter in chicory (Cichorium intybus L.)

Liang

Lin

et al. 2014

Plant Growth Regul

View full text Add to dashboard Cite

show abstract

“…The vacuole membrane-localized Na + /H + reverse transporters of plants play a key role in improving their tolerance to salt stress. Overexpression of NHX-type genes, including SsNHX1, improves the salt tolerance of the transgenic plants of many species (Apse et al 1999;Masaru et al 2002;Lu et al 2014). Considering the limited information on SsNHX1 in terms of the genetic improvement of crops, additional studies on the utilization of SsNHX1 to improve the salt tolerance of maize are urgently needed.…”

Section: Discussionmentioning

confidence: 99%

“…For example, the AgNHX1 gene of the halophytic plant Atriplex gmelini allows transgenic rice (Oryza sativa) to survive high NaCl concentrations (Masaru et al 2002). Lu et al (2014) reported that overexpression of TaNHX3, a wheat (Triticum aestivum) vacuolar NHX gene, enhanced salt tolerance in tobacco by improving related physiological processes, indicating that TaNHX3 plays an important role in regulating cytosolic Na + transport into vacuoles. These results suggest that vacuolar NHX not only reduces the toxicity caused by high concentrations of Na + but also maintains the ionic balance of the plant cell.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of the Suaeda salsa SsNHX1 gene confers enhanced salt and drought tolerance to transgenic Zea mays

Huang

Zhang

et al. 2018

Journal of Integrative Agriculture

View full text Add to dashboard Cite

Maize is one of the most important crops worldwide, but it suffers from salt stress when grown in saline-alkaline soil. There is therefore an urgent need to improve maize salt tolerance and crop yield. In this study, the SsNHX1 gene of Suaeda salsa, which encodes a vacuolar membrane Na + /H + antiporter, was transformed into the maize inbred line 18-599 by Agrobacterium-mediated transformation. Transgenic maize plants overexpressing the SsNHX1 gene showed less growth retardation when treated with an increasing NaCl gradient of up to 1%, indicating enhanced salt tolerance. The improved salt tolerance of transgenic plants was also demonstrated by a significantly elevated seed germination rate (79%) and a reduction in seminal root length inhibition. Moreover, transgenic plants under salt stress exhibited less physiological damage. SsNHX1-overexpressing transgenic maize accumulated more Na + and K + than wild-type (WT) plants particularly in the leaves, resulting in a higher ratio of K + /Na + in the leaves under salt stress. This result revealed that the improved salt tolerance of SsNHX1-overexpressing transgenic maize plants was likely attributed to SsNHX1-mediated localization of Na + to vacuoles and subsequent maintenance of the cytosolic ionic balance. In addition, SsNHX1 overexpression also improved the drought tolerance of the transgenic maize plants, as rehydrated transgenic plants were restored to normal growth while WT plants did not grow normally after dehydration treatment. Therefore, based on our engineering approach, SsNHX1 represents a promising candidate gene for improving the salt and drought tolerance of maize and other crops.

show abstract

“…SOS1 and NHX1 are considered to be important in controlling ion homeostasis in the cytoplasm during salinity stress. Recent studies show that enhanced activity of SOS1 in Arabidopsis thaliana (arabidopsis) increases salt tolerance (Feki et al, ) and overexpression of Triticum aestivum (wheat) NHX in Nicotiana tabacum (tobacco) also increases plant performance under salinity stress (Lu et al, ). Another target of the SOS2/3 complex is the vacuolar H + ‐ATPase (V‐ATPase), a common H + pump in plant cells (Batelli et al, ; Dietz et al, ).…”

Section: Adaptive Mechanisms and Signalling During Salt Exposurementioning

confidence: 99%

Connecting salt stress signalling pathways with salinity‐induced changes in mitochondrial metabolic processes inC3 plants

Che-Othman

Millar

Taylor

2017

Plant Cell & Environment

View full text Add to dashboard Cite

Salinity exerts a severe detrimental effect on crop yields globally. Growth of plants in saline soils results in physiological stress, which disrupts the essential biochemical processes of respiration, photosynthesis, and transpiration. Understanding the molecular responses of plants exposed to salinity stress can inform future strategies to reduce agricultural losses due to salinity; however, it is imperative that signalling and functional response processes are connected to tailor these strategies. Previous research has revealed the important role that plant mitochondria play in the salinity response of plants. Review of this literature shows that 2 biochemical processes required for respiratory function are affected under salinity stress: the tricarboxylic acid cycle and the transport of metabolites across the inner mitochondrial membrane. However, the mechanisms by which components of these processes are affected or react to salinity stress are still far from understood. Here, we examine recent findings on the signal transduction pathways that lead to adaptive responses of plants to salinity and discuss how they can be involved in and be affected by modulation of the machinery of energy metabolism with attention to the role of the tricarboxylic acid cycle enzymes and mitochondrial membrane transporters in this process. Saline landscapes occur naturally, and many halophytes (salt tolerant plants) survive and complete their life cycle in these environments (Flowers & Colmer, 2015). Human activity including intense irrigation and land clearing can also cause soil salinity, as these activities lead to changes in the soil water table, which can contain high salt concentrations (Rengasamy, 2006). These agricultural practices result in a rising water table, which liberates soil solutes bringing them to the surface, creating salinized areas in previously productive land (Rengasamy, 2006). This severely limits crop production in these areas as typically elite high yielding crop varieties cannot tolerate these high salt concentrations (Roy, Negrão, & Tester, 2014).Although the salinization of soils has and is increasingly limiting crop production in many areas of the world (Sha Valli Khan, Nagamallaiah, Dhanunjay, Sergeant, & Hausman, 2014), reliable measures of salt-affected land are very difficult to obtain because salinity varies both spatially and temporally. Nevertheless, a number of studies have attempted to estimate the extent of soil salinity.According to the Food and Agriculture Organisation of the United Nations, it is estimated that approximately 20% of all global irrigated land has become salt-affected and this percentage is expected to continue to increase over time (FAO, 2009;Parihar, Singh, Singh, Singh, & Prasad, 2014). It was further estimated that this salinization of irrigated --------------------------------------------------This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium...

show abstract

Overexpression of TaNHX3, a vacuolar Na+/H+ antiporter gene in wheat, enhances salt stress tolerance in tobacco by improving related physiological processes

Cited by 52 publications

References 44 publications

Identification, functional characterization, and expression pattern of a NaCl-inducible vacuolar Na+/H+ antiporter in chicory (Cichorium intybus L.)

Identification, functional characterization, and expression pattern of a NaCl-inducible vacuolar Na+/H+ antiporter in chicory (Cichorium intybus L.)

Overexpression of the Suaeda salsa SsNHX1 gene confers enhanced salt and drought tolerance to transgenic Zea mays

Connecting salt stress signalling pathways with salinity‐induced changes in mitochondrial metabolic processes inC3 plants

Contact Info

Product

Resources

About