Overexpression of the alfalfa WRKY11 gene enhances salt tolerance in soybean

Wang, Youjing; Jiang, Lin; Chen, Jiaqi; Lei, Tao; An, Yimin; Cai, Hongsheng; Guo, Changhong

doi:10.1371/journal.pone.0192382

Cited by 57 publications

(30 citation statements)

References 61 publications

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“…Accumulation of MDA content can lead to membrane lipid peroxidation of plant cells, causing changes in the cell membrane structure and permeability, reducing cell function [ 47 ]. In contrast, proline prevents membrane lipid peroxidation, maintains normal cellular structure, and maintains a stable cell osmotic pressure [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Chrysanthemum DgWRKY2 Gene Enhances Tolerance to Salt Stress in Transgenic Chrysanthemum

Zhao

et al. 2018

IJMS

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WRKY transcription factors (TFs) play a vital part in coping with different stresses. In this study, DgWRKY2 was isolated from Dendranthema grandiflorum. The gene encodes a 325 amino acid protein, belonging to the group II WRKY family, and contains one typical WRKY domain (WRKYGQK) and a zinc finger motif (C-X4-5-C-X22-23-H-X1-H). Overexpression of DgWRKY2 in chrysanthemum enhanced tolerance to high-salt stress compared to the wild type (WT). In addition, the activities of antioxidant enzymes (superoxide dismutase (SOD), peroxidase (POD), catalase (CAT)), proline content, soluble sugar content, soluble protein content, and chlorophyll content of transgenic chrysanthemum, as well as the survival rate of the transgenic lines, were on average higher than that of the WT. On the contrary, hydrogen peroxide (H2O2), superoxide anion (O2−), and malondialdehyde (MDA) accumulation decreased compared to WT. Expression of the stress-related genes DgCAT, DgAPX, DgZnSOD, DgP5CS, DgDREB1A, and DgDREB2A was increased in the DgWRKY2 transgenic chrysanthemum compared with their expression in the WT. In conclusion, our results indicate that DgWRKY2 confers salt tolerance to transgenic chrysanthemum by enhancing antioxidant and osmotic adjustment. Therefore, this study suggests that DgWRKY2 could be used as a reserve gene for salt-tolerant plant breeding.

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Section: Discussionmentioning

confidence: 99%

Chrysanthemum DgWRKY2 Gene Enhances Tolerance to Salt Stress in Transgenic Chrysanthemum

Zhao

et al. 2018

IJMS

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“…Salinity stress has a marked negative effect on its germination, biomass production, forage quality and nitrogen fixation[ 10 , 11 ]. The obligate outcrossing and allotetraploid character (2n = 4x = 32) makes it carry a large genetic diversity ranging from susceptible to tolerant to moderate salinity among different cultivars[ 12 – 14 ]. The adaptive mechanism of alfalfa under abiotic stress is a complex multigenic process that is not well understood.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional profiling reveals that a MYB transcription factor MsMYB4 contributes to the salinity stress response of alfalfa

Wang

Liu

et al. 2018

PLoS ONE

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MYB transcription factors are important regulators of the plant response to abiotic stress. Their participation in the salinity stress of the key forage legume species alfalfa (Medicago sativa) was investigated here by comparing the transcriptomes of the two cultivars Dryland (DL) and Sundory (SD), which differed with respect to their ability to tolerate salinity stress. When challenged by the stress, DL plants were better able than SD ones to scavenge reactive oxygen species. A large number of genes encoding transcription regulators, signal transducers and proteins involved in both primary and secondary metabolism were differentially transcribed in the two cultivars, especially when plants were subjected to salinity stress. The set of induced genes included 17 MYB family of transcription factors, all of which were subsequently isolated. The effect of constitutively expressing these genes on the salinity tolerance expressed by Arabidopsis thaliana was investigated. The introduction of MsMYB4 significantly increased the plants’ salinity tolerance in an abscisic acid-dependent manner. A sub-cellular localization experiment and a transactivation assay indicated that MsMYB4 was deposited in the nucleus and was able to activate transcription in yeast. Based on this information, we propose that the MsMYB4 products is likely directly involved in alfalfa’s response to salinity stress.

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“…Breeding new alfalfa cultivars with high salt tolerance is always needed. Genetic engineering and conventional breeding have been proven efficient for improving salt tolerance of various plant species [10][11][12][13]. However, they are time-consuming and complicated.…”

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confidence: 99%

Melatonin Application Improves Salt Tolerance of Alfalfa (Medicago sativa L.) by Enhancing Antioxidant Capacity

Cen

Wang

Liu

et al. 2020

Plants

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Alfalfa (Medicago sativa L.) is an important and widely cultivated forage grass. The productivity and forage quality of alfalfa are severely affected by salt stress. Melatonin is a bioactive molecule with versatile physiological functions and plays important roles in response to various biotic and abiotic stresses. Melatonin has been proven efficient in improving alfalfa drought and waterlogging tolerance in recent studies. In our reports, we applied melatonin exogenously to explore the effects of melatonin on alfalfa growth and salt resistance. The results demonstrated that melatonin application promoted alfalfa seed germination and seedling growth, and reduced oxidative damage under salt stress. Further application research found that melatonin alleviated salt injury in alfalfa plants under salt stress. The electrolyte leakage, malondialdehyde (MDA) content and H 2 O 2 content were significantly reduced, and the activities of catalase (CAT), peroxidase (POD), and Cu/Zn superoxide dismutase (Cu/Zn-SOD) were increased with melatonin pretreatment compared to control plants under salt stress with the upregulation of genes related to melatonin and antioxidant enzymes biosynthesis. Melatonin was also involved in reducing Na + accumulation in alfalfa plants. Our study indicates that melatonin plays a primary role as an antioxidant in scavenging H 2 O 2 and enhancing activities of antioxidant enzymes to improve the salt tolerance of alfalfa plants.Plants 2020, 9, 220 2 of 17 world's irrigated land affected by saline or sodic globally [7,8]. Salinity has become one of the most important environmental stress factors impairing worldwide agricultural productivity. Under salinity conditions, the seed germination, growth and development processes of alfalfa are inhibited and they finally impair the herbage yield, as well as the forage quality [9]. Breeding new alfalfa cultivars with high salt tolerance is always needed. Genetic engineering and conventional breeding have been proven efficient for improving salt tolerance of various plant species [10][11][12][13]. However, they are time-consuming and complicated. Exogenous application of certain plant growth regulators such as phytohormone and other small molecules has been proven efficient at overcoming the harmful effects of salt stress on plants [14][15][16][17]. Moreover, plant growth regulators in low concentrations always play a role in plants, and are cost-effective. Foliar spraying of salicylic acid (SA) on faba bean inhibits Na + accumulation and lipid peroxidation, improving the antioxidant resistance and finally mitigating the damage caused by salinity [18]. Exogenously applied poly-γ-glutamic acid on wheat maintains the Na + and K + homeostasis and enhances antioxidant capacity by alleviating salinity damage under salt stress conditions [19]. Exogenous spermidine application to salt-stressed cucumber improves the photosynthetic capacity and the activity of key enzymes for CO 2 fixation by regulating the expression of related genes, and tolerance to salinity is thus...

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Overexpression of the alfalfa WRKY11 gene enhances salt tolerance in soybean

Cited by 57 publications

References 61 publications

Chrysanthemum DgWRKY2 Gene Enhances Tolerance to Salt Stress in Transgenic Chrysanthemum

Chrysanthemum DgWRKY2 Gene Enhances Tolerance to Salt Stress in Transgenic Chrysanthemum

Transcriptional profiling reveals that a MYB transcription factor MsMYB4 contributes to the salinity stress response of alfalfa

Melatonin Application Improves Salt Tolerance of Alfalfa (Medicago sativa L.) by Enhancing Antioxidant Capacity

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