Overexpression of the Arabidopsis H+-PPase enhanced resistance to salt and drought stress in transgenic alfalfa (Medicago sativa L.)

Bao, Ai-Ke; Wang, Suo-Min; Wu, Guoqiang; Xi, Jing; Zhang, Jin‐Lin; Wang, Chunmei

doi:10.1016/j.plantsci.2008.10.009

Cited by 217 publications

(133 citation statements)

References 38 publications

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“…The salt-tolerant phenotype was explained by an increased capacity for Na + uptake into vacuoles, and drought resistance was attributed to an enhanced vacuolar osmoregulatory capacity (Gaxiola et al, 2001(Gaxiola et al, , 2002. It has been subsequently demonstrated that overexpression of AVP1 and other plant type I H + -PPase genes can increase both salt and drought tolerance in diverse systems, including tobacco (Nicotiana tabacum; Gao et al, 2006), cotton (Gossypium hirsutum; Lv et al, 2008Lv et al, , 2009, alfalfa (Medicago sativa; Bao et al, 2008), maize (Zea mays; Li et al, 2008), and creeping bentgrass (Agrostis stolonifera; Li et al, 2010). Interestingly, a study on the variation of salinity tolerance in Arabidopsis ecotypes reported a positive relationship between salt tolerance and AVP1 expression (Jha et al, 2010).…”

Section: Enhanced Salt Tolerance Is An Anticipated Phenotype Triggerementioning

confidence: 99%

Genetic Manipulation of a “Vacuolar” H+-PPase: From Salt Tolerance to Yield Enhancement under Phosphorus-Deficient Soils

et al. 2012

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Section: Enhanced Salt Tolerance Is An Anticipated Phenotype Triggerementioning

confidence: 99%

Genetic Manipulation of a “Vacuolar” H+-PPase: From Salt Tolerance to Yield Enhancement under Phosphorus-Deficient Soils

et al. 2012

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“…The absorbance at 450, 532, and 600 nm (A 450 , A 532 , and A 600 , respectively) was determined using an ultraviolet spectrophotometer (UV-2102C, Unico Instrument Co., Shanghai, China). The content of MDA in nmol/g FW was calculated according to the following equation as described by Bao et al (2009):…”

Section: Plantmentioning

confidence: 99%

Response of growth and antioxidant enzymes to osmotic stress in two different wheat (Triticum aestivum L.) cultivars seedlings

Wu¹,

Zhang²,

Wang³

2012

Plant Soil Environ.

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To investigate the responses of growth and antioxidant enzymes to osmotic stress in two different wheat cultivars, one drought tolerant (Heshangtou, HST) and the other drought sensitive (Longchun 15, LC15), 15-day-old wheat seedlings were exposed to osmotic stress of -0.25, -0.50, and -0.75 MPa for 2 days. It is found that osmotic stress decreased shoot length in both wheat cultivars, whereas to a lesser degree in HST than in LC15. The contents of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) of shoot in both wheat cultivars were increased by osmotic stress. It is clear that MDA contents increased less in the more drought tolerant cultivar HST than in drought sensitive one LC15. On the contrary, POD and CAT activities increased more in HST than LC15 under osmotic stress. As the activity of SOD, however, no significant differences were found between HST and LC15. These results suggest that wheat cultivar HST has higher activities of antioxidant enzymes such as POD and CAT to cope with oxidative damage caused by osmotic stress compared to sensitive LC15.

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“…Gara-Yonjeh, with amplification conditions of an initial denaturation at 94 °C for 5 min, followed by 30 cycles of 94°C for 60 s, 55 °C for 60 s, 72 °C for 120 s, then a final extension at 72 °C for 10 min. A 286bp fragment of alfalfa species glyceraldehyde-3-phosphate dehydrogenase (GPDH) gene, used as an internal control, was amplified with the specific primers: 5´-GTGGTGCCAAGAAGGTTGTTAT-3´ and 5´-CTGGGAATGATGTTGAAGGAAG-3´ (Bao et al 2009 °C for 8 min. The PCR products were electrophoresed on a 1.5 percent agarose gel containing ethidium bromide.…”

Section: Methodsmentioning

confidence: 99%

A NOVEL BETAINE ALDEHYDE DEHYDROGENASE GENE FROM Medicago sativa AND ITS EXPRESSION UNDER SALINITY

Amjad¹,

Nosrati²,

Zaare³

et al. 2015

AgricultForest

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SUMMARYBetaine aldehyde dehydrogenase (BADH) has been reported from several plant species mostly halophytes as the most important enzyme responsible for salinity tolerance. We investigated the expression of BADH gene along with ionic and enzymatic accumulations in an alfalfa cultivar, Medicago sativa cv. Gara-Yonjeh, widely cultivated in saline soils across East-Azerbaijan Province, Iran. Five different NaCl concentrations of 0, 50, 100, 150 and 200 mM were examined using 30 seeds for each treatment. The Na + , K + , Ca 2+ and proline concentration were measured in cotyledon and young roots of seven-day-old seedlings under salinity stresses and controls. Total RNA was extracted and the mRNA levels of P5CS2 (∆ 1 -pyrroline-5-carboxylate synthetase) and BADH (hereafter MsP5CS2 and MsBD1, respectively) were determined using semiquantitative RT-PCR analysis. MsBD1mRNA was reverse-transcribed and then was amplified using PCR. The amplified MsBD1 DNAs were cloned. Our results showed that 150mM NaCl was the stress threshold concentration for the young seedlings. The accumulation of the cations and proline significantly increased by increasing NaCl concentration from 0 to 150mM. The mRNA levels of MsP5CS2 and MsBD1 increased in both cotyledons and roots at 150mM compared to control. A 1518 basepair cDNA fragment coding for MsBD1, and its proposed 505-amino acid sequences were characterized in Medicago sativa cv. GaraYonjeh. These DNA and amino acid sequences showed high homology with BADH gene/amino acid sequences previously reported from several other plant species. Our study shows that genic and ionic mechanisms are simultaneously employed in this cultivar to cope with salinity.

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Overexpression of the Arabidopsis H+-PPase enhanced resistance to salt and drought stress in transgenic alfalfa (Medicago sativa L.)

Cited by 217 publications

References 38 publications

Genetic Manipulation of a “Vacuolar” H+-PPase: From Salt Tolerance to Yield Enhancement under Phosphorus-Deficient Soils

Genetic Manipulation of a “Vacuolar” H+-PPase: From Salt Tolerance to Yield Enhancement under Phosphorus-Deficient Soils

Response of growth and antioxidant enzymes to osmotic stress in two different wheat (Triticum aestivum L.) cultivars seedlings

A NOVEL BETAINE ALDEHYDE DEHYDROGENASE GENE FROM Medicago sativa AND ITS EXPRESSION UNDER SALINITY

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