Overexpression of the celA1 gene in BCG modifies surface pellicle, glucosamine content in biofilms, and affects in vivo replication

Vaca-González, Alonso; Flores-Valdéz, Mario Alberto; Aceves-Sánchez, Michel de Jesús; Camacho-Villegas, Tanya A.; Pérez-Padilla, Nayeli Areli; Burciaga-Flores, Mirna; Cruz, Miguel A. De la; Ares, Miguel A.; Mora-Montes, Héctor M.; Madrigal, Jorge; Gaona-Bernal, Jorge; Tamez-Castrellón, Alma K.

doi:10.1016/j.tube.2020.102005

Cited by 2 publications

(1 citation statement)

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“…Furthermore, deletion of the mbtB gene results in the limited growth of M. tuberculosis H37Rv in iron-limited media, but normal growth in iron-replete media ( De Voss et al., 2000 ). celA1 belongs to glycosyl hydrolase family 6 with cellulase function ( Vaca-Gonzalez et al., 2020 ). Cellulose is an important component of the biofilm matrix and is involved in biofilm formation ( Limoli et al., 2015 ).…”

Section: Discussionmentioning

confidence: 99%

mbtD and celA1 association with ethambutol resistance in Mycobacterium tuberculosis: A multiomics analysis

Tan²,

Zhang

et al. 2022

Front. Cell. Infect. Microbiol.

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Ethambutol (EMB) is a first-line antituberculosis drug currently being used clinically to treat tuberculosis. Mutations in the embCAB operon are responsible for EMB resistance. However, the discrepancies between genotypic and phenotypic EMB resistance have attracted much attention. We induced EMB resistance in Mycobacterium tuberculosis in vitro and used an integrated genome–methylome–transcriptome–proteome approach to study the microevolutionary mechanism of EMB resistance. We identified 509 aberrantly methylated genes (313 hypermethylated genes and 196 hypomethylated genes). Moreover, some hypermethylated and hypomethylated genes were identified using RNA-seq profiling. Correlation analysis revealed that the differential methylation of genes was negatively correlated with transcription levels in EMB-resistant strains. Additionally, two hypermethylated candidate genes (mbtD and celA1) were screened by iTRAQ-based quantitative proteomics analysis, verified by qPCR, and corresponded with DNA methylation differences. This is the first report that identifies EMB resistance-related genes in laboratory-induced mono-EMB-resistant M. tuberculosis using multi-omics profiling. Understanding the epigenetic features associated with EMB resistance may provide new insights into the underlying molecular mechanisms.

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Section: Discussionmentioning

confidence: 99%