Overexpression of theArabidopsisvacuolar H+-pyrophosphataseAVP1gene in rice plants improves grain yield under paddy field conditions

Kim, Y. S.; Kim, I. S.; Choe, Yong‐Hoe; Bae, Mi‐Jung; Shin, Sun‐Young; Park, S. K.; Kang, Hong‐Gyu; Kim, Y. H.; Yoon, Ho-Sung

doi:10.1017/s0021859613000671

Cited by 10 publications

(3 citation statements)

References 54 publications

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“…During grain filling, GM plants showed an increase of 61.4% in photosynthetic rate, a reduction of 23% in internal carbon concentration, and an increase of 89.6% in carboxylation efficiency. Other authors also found the highest photosynthetic rate during grain filling due to the overexpression of the OVP1 gene in A. thaliana (Khadilkar et al, 2016), rice (Kim et al, 2014), cotton (Gossypium hirsutum L.) (Pasapula et al, 2011), and sugarcane (Saccharum officinarum L.) (Raza et al, 2016). However, unlike in cotton and sugarcane, the overexpression of the OVP1 gene in the GM rice plants evaluated in the present study did not show significant changes in stomatal conductance and transpiration rate.…”

Section: Resultssupporting

confidence: 47%

Physiological and phenotypical effects of the overexpression of the OVP1 gene in Japonica rice

Rocha,

Cruz,

Oliveira

et al. 2023

Pesq. agropec. bras.

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The objective of this work was to evaluate the physiological, phenotypic, and gene expression parameters in genetically modified (GM) rice plants that overexpress the Oryza sativa Vacuolar H+-Pyrophosphatase 1 (OVP1) gene, compared with non-genetically modified (NGM) rice. GM and NGM plants of the BRSMG Curinga cultivar were evaluated in two experiments, in a laboratory and greenhouse, in a randomized complete block design, with four replicates. Agronomic traits of interest were estimated, and transcriptome analysis and gene expression quantification were carried out. GM plants showed a 31 and 21% higher number of spikelets per panicle and total number of grains per panicle, respectively, in comparison with NGM plants. Physiological changes occurred during the grain-filling stage, in which GM plants presented a photosynthetic rate and carboxylation efficiency 61 and 89% higher than those of NGM plants, respectively. The overexpression of the OVP1 gene favors the upregulation of some photosynthesis genes and the increase in the number of spikelets and in the photosynthetic rate, but does not favor the increase in grain yield.

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Section: Resultssupporting

confidence: 47%

Physiological and phenotypical effects of the overexpression of the OVP1 gene in Japonica rice

Rocha,

Cruz,

Oliveira

et al. 2023

Pesq. agropec. bras.

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“…The functional characterization of AVP1 in A. thaliana has been established by studies in mutants and overexpression lines. Some of its known roles are regulation of auxin-mediated organ development and involvement in various abiotic stress tolerance ( Li et al., 2005 ; Kim et al., 2014 ; Nepal et al., 2020 ; Zhang et al., 2023 ). It has also been shown that rather than proton pumping, PPi hydrolysis activity is the major contribution of AVP1 in organ development as demonstrated using fugu5 mutants ( Ferjani et al., 2011 ; Asaoka et al., 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Mutations in type II Golgi-localized proton pyrophosphatase AVP2;1/VHP2;1 affect pectic polysaccharide rhamnogalacturonan-II and alter root growth under low boron condition in Arabidopsis thaliana

Onuh,

Miwa

2023

Front. Plant Sci.

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The essential plant nutrient boron is required for the crosslinking of the pectin polysaccharide, rhamnogalacturonan II (RG-II). The synthesis of the pectic polysaccharides takes place in the Golgi apparatus, acidified by proton pumps. AVP2;1/VHP2;1 is a type II proton pyrophosphatase localized in the Golgi apparatus, which possesses proton pumping activity coupled with pyrophosphate hydrolysis. Its activity and expression patterns have been previously revealed but its role in plants remains unknown. The aim of the present work therefore was to explore the physiological role of AVP2;1 in Arabidopsis thaliana. In the screening of mutants under low boron, a mutant carrying a missense mutation in AVP2;1 was isolated. This mutant showed increased primary root growth under low boron conditions but no significant difference under normal boron condition compared to wild type plants. T-DNA insertion caused similar growth, suggesting that reduced function of AVP2;1 was responsible. Root cell observation revealed an increase in meristematic zone length, cell number in meristem and length of matured cell in avp2;1 mutants compared to wild type under low boron. Calcium concentration was reduced in mutant root cell wall under low boron. RG-II specific sugars also tended to be decreased in mutant root cell wall under low and normal boron conditions. These results suggest that changes in cell wall component by mutations in AVP2;1 may possibly explain the increased root length of mutants under low boron. This supports the idea that AVP2;1 plays a role in pH homoeostasis in Golgi apparatus for pectin synthesis.

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“…A variety of roles for these enzymes, particularly the type I H + ‐PPases, have been proposed (Gaxiola et al, 2016 ; Schilling et al, 2017 ), including aiding vacuolar ion sequestration (Gaxiola et al, 1999 , 2001 ) and sugar transportation (Gaxiola et al, 2012 ; Khadilkar et al, 2016 ; Pizzio et al, 2015 ), regulating cytosolic PP i (Ferjani et al, 2011 ) and assisting with nutrient uptake (Paez‐Valencia et al, 2013 ; Yang et al, 2007 , 2014 ). Numerous studies have shown that transgenic plants expressing H + ‐PPases have increased biomass and yield under saline (Bao et al, 2009 ; Cheng et al, 2018 ; Gaxiola et al, 2001 ; Kim et al, 2013 ; Li et al, 2010 ; Pasapula et al, 2011 ; Qin et al, 2013 ; Schilling et al, 2014 ; Shen et al, 2015 ), drought (Bao et al, 2009 ; Gaxiola et al, 2001 ; Park et al, 2005 ; Qin et al, 2013 ; Shen et al, 2015 ), low available nitrogen (Lv et al, 2015 ; Paez‐Valencia et al, 2013 ), and low available phosphorus (Yang et al, 2007 , 2014 ) conditions, as well as in non‐stressed conditions (Gonzalez et al, 2010 ; Li et al, 2005 ; Pizzio et al, 2015 ; Schilling et al, 2014 ; Vercruyssen et al, 2011 ; Wang et al, 2014 ; Yang et al, 2014 ). Recently, it was shown that expression of Arabidopsis AVP1 in bread wheat enhanced biomass production and yield in both field and greenhouse conditions (Regmi et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Proton‐pumping pyrophosphatase homeolog expression is a dynamic trait in bread wheat (Triticum aestivum)

et al. 2021

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Proton‐pumping pyrophosphatases (H + ‐PPases) have been shown to enhance biomass and yield. However, to date, there has been little work towards identify genes encoding H + ‐PPases in bread wheat ( Triticum aestivum ) ( TaVP s) and limited knowledge on how the expression of these genes varies across different growth stages and tissue types. In this study, the IWGSC database was used to identify two novel TaVP genes, TaVP4 and TaVP5 , and elucidate the complete homeolog sequences of the three known TaVP genes, bringing the total number of bread wheat TaVP s from 9 to 15. Gene expression levels of each TaVP homeolog were assessed using quantitative real‐time PCR (qRT‐PCR) in four diverse wheat varieties in terms of phenotypic traits related to high vacuolar pyrophosphatase expression. Homeolog expression was analyzed across multiple tissue types and developmental stages. Expression levels of the TaVP homeologs were found to vary significantly between varieties, tissues and plant developmental stages. During early development (Z10 and Z13), expressions of TaVP1 and TaVP2 homeologs were higher in shoot tissue than root tissue, with both shoot and root expression increasing in later developmental stages (Z22). TaVP2‐D was expressed in all varieties and tissue types and was the most highly expressed homeolog at all developmental stages. Expression of the TaVP3 homeologs was restricted to developing grain (Z75), while TaVP4 homeolog expression was higher at Z22 than earlier developmental stages. Variation in TaVP4B was detected among varieties at Z22 and Z75, with Buck Atlantico (high biomass) and Scout (elite Australian cultivar) having the highest levels of expression. These findings offer a comprehensive overview of the bread wheat H + ‐PPase family and identify variation in TaVP homeolog expression that will be of use to improve the growth, yield, and abiotic stress tolerance of bread wheat.

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Overexpression of theArabidopsisvacuolar H⁺-pyrophosphataseAVP1gene in rice plants improves grain yield under paddy field conditions

Cited by 10 publications

References 54 publications

Physiological and phenotypical effects of the overexpression of the OVP1 gene in Japonica rice

Physiological and phenotypical effects of the overexpression of the OVP1 gene in Japonica rice

Mutations in type II Golgi-localized proton pyrophosphatase AVP2;1/VHP2;1 affect pectic polysaccharide rhamnogalacturonan-II and alter root growth under low boron condition in Arabidopsis thaliana

Proton‐pumping pyrophosphatase homeolog expression is a dynamic trait in bread wheat (Triticum aestivum)

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