Overexpression of the laccase gene, lcc1, in Lentinula edodes using the pChG vector

Sato, Toshitsugu; Suzuki, Y.; Naito, Makio; Minami, Aika; Suzuki, Naoyuki; Yaegashi, Kaori; Hirano, Takeshi

doi:10.1016/j.myc.2019.02.004

Cited by 4 publications

(4 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Expression of glyceraldehyde 3phosphate dehydrogenase (GAPDH) gene was used as an internal reference for normalizing the qRT-PCR data. 27 Three independent replicates were adopted for testing each gene. The sequences of primers used for qRT-PCR are listed in Table 1.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Functions of a Glucan Synthase Gene GFGLS in Mycelial Growth and Polysaccharide Production of Grifola frondosa

Cui

Tao

et al. 2019

J. Agric. Food Chem.

View full text Add to dashboard Cite

Glucan synthase (GLS) gene is known to be involved in the fungal biosynthesis of cell wall, differentiation, and growth. In the present study, a glucan synthase gene (GFGLS) in the edible mushroom Grifola frondosa with a full sequence of 5927 bp encoding a total of 1781 amino acids was cloned and characterized for the first time. GFGLSp is a membrane protein containing two large transmembrane domains connected with a hydrophilic cytoplasmic domain. With a constructed dual promoter RNA silencing vector pAN7-gfgls-dual, a GFGLS-silencing transformant iGFGLS-3 had the lowest GFGLS transcriptional expression level (26.1%) with a shorter length and thinner appearance of the mycelia, as well as decreased mycelial biomass and exo-polysaccharide production of 5.02 and 0.38 g/L, respectively. Further analysis indicated that GFGLS silence influenced slightly the monosaccharide compositions and ratios of mycelial and exo-polysaccharide. These findings suggest that GFGLS could affect mycelial growth and polysaccharide production by downregulating the glucan synthesis.

show abstract

Section: Methodsmentioning

confidence: 99%

“…Relative quantification was carried out using the 2 –ΔΔCt method. Expression of glyceraldehyde 3-phosphate dehydrogenase ( GAPDH ) gene was used as an internal reference for normalizing the qRT-PCR data . Three independent replicates were adopted for testing each gene.…”

Section: Methodsmentioning

confidence: 99%

Functions of a Glucan Synthase Gene GFGLS in Mycelial Growth and Polysaccharide Production of Grifola frondosa

Cui

Tao

et al. 2019

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…Affect the elongation of fruiting bodies and the formation of primordia [162] vvaao1 Aryl Alcohol Oxidase Promote the formation and development of fruiting bodies [163] Lentinula edodes lcc1 Laccase Involved in fruiting body growth and development [164] Auricularia cornea AcveA Velvet factor family protein Regulation of fruiting body pigment synthesis [165] Pholiota microspora PnGcs α-glucosidase Involved in fruiting body development [166] Griflola frondosa Rho1 β-1,3-glucan synthase Promote mycelium growth [167] Hydrophobic proteins are involved in multiple developmental processes in edible and medicinal fungi and play an important role in spore spread, pathogenesis, and the formation of ectomycorrhiza. Tao et al [136] used interference methods to identify the function of Hyd9 in F. filiformis and reported that the surfaces of the aerated mycelia were dense after overexpression, while the surfaces of the silenced mycelia were sparse, suggesting that the hydrophobic protein could help fungi form aerated mycelia.…”

Section: Vvhox1-vvhox8mentioning

confidence: 99%

Current Advances in the Functional Genes of Edible and Medicinal Fungi: Research Techniques, Functional Analysis, and Prospects

Li,

Zou,

Bao

et al. 2024

JoF

View full text Add to dashboard Cite

Functional genes encode various biological functions required for the life activities of organisms. By analyzing the functional genes of edible and medicinal fungi, varieties of edible and medicinal fungi can be improved to enhance their agronomic traits, growth rates, and ability to withstand adversity, thereby increasing yield and quality and promoting industrial development. With the rapid development of functional gene research technology and the publication of many whole-genome sequences of edible and medicinal fungi, genes related to important biological traits have been mined, located, and functionally analyzed. This paper summarizes the advantages and disadvantages of different functional gene research techniques and application examples for edible and medicinal fungi; systematically reviews the research progress of functional genes of edible and medicinal fungi in biological processes such as mating type, mycelium and fruit growth and development, substrate utilization and nutrient transport, environmental response, and the synthesis and regulation of important active substances; and proposes future research directions for functional gene research for edible and medicinal fungi. The overall aim of this study was to provide a valuable reference for further promoting the molecular breeding of edible and medicinal fungi with high yield and quality and to promote the wide application of edible and medicinal fungi products in food, medicine, and industry.

show abstract

“…Mishra, 2020;Ahlawat and Mishra, 2022) Sato et al (2019). utilized Lentinula edodes to produce the laccase for industrial manufacture, which was veri ed the lcc1 gene was expressed and then promoted biosynthesis of laccase Yan et al (2019).…”

mentioning

confidence: 99%

Enhancement of hyphae growth and medium optimization by Pleurotus eryngii-3 under submerged fermentation

Liu

Feng

Wang

et al. 2022

Preprint

View full text Add to dashboard Cite

There were a variety of amino acids and polysaccharides in Pleurotus eryngii contained that could improve human immunity and prevent cancer. In this paper, the medium was screened and carton and nitrogen sources were screened and optimized as 40 g/L corn flour and soybean meal by P. eryngii-3. Then, the effect of pH, temperature, volume, and rotate speed were investigated, in which the suitable fermentation conditions were respectively pH = 6, 27 ℃, 90 mL of fermentation broth, and 150 r/min of rotate speed. Meanwhile, the transcription levels of lcc1, lcc5, lcc8, and lcc12 were up-regulated by 10.25-, 3.32-, 2.33- and 6.29-fold respectively after optimization. In addition, the box-behnken design was utilized to improve DCW (17.23 g/L). This research was optimized the medium and condition of fermentation by P. eryngii-3, which could shorten the ripening cycle of seeds and improve economic benefits for industries.

show abstract

Overexpression of the laccase gene, lcc1, in Lentinula edodes using the pChG vector

Cited by 4 publications

References 12 publications

Functions of a Glucan Synthase Gene GFGLS in Mycelial Growth and Polysaccharide Production of Grifola frondosa

Functions of a Glucan Synthase Gene GFGLS in Mycelial Growth and Polysaccharide Production of Grifola frondosa

Current Advances in the Functional Genes of Edible and Medicinal Fungi: Research Techniques, Functional Analysis, and Prospects

Enhancement of hyphae growth and medium optimization by Pleurotus eryngii-3 under submerged fermentation

Contact Info

Product

Resources

About