Overexpression of the Monodehydroascorbate Reductase Gene from Non-heading Chinese Cabbage Reduces Ascorbate Level and Growth in Transgenic Tobacco

Ren, Jun; Duan, Weike; Chen, Zhongwen; Zhang, Shuo; Song, Xiaoming; Hou, Xilin; Hou, Xilin; Li, Ying

doi:10.1007/s11105-014-0797-y

Cited by 11 publications

(4 citation statements)

References 44 publications

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“…Although MDHAR also governs AsA regeneration, the expression level of MDHAR does not always coincide with AsA content. Transgenic plans are overexpressing MDHAR to decrease AsA accumulation, while knockdown of MDHAR gene decreases the AsA content [56,58,59]. However, overexpression of acerola MDHAR in tobacco significantly increases AsA content [60].…”

Section: Regeneration and Degradation Of Ascorbic Acidmentioning

confidence: 99%

Metabolism and Regulation of Ascorbic Acid in Fruits

Zheng

Gong

Zhang

et al. 2022

Plants

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Ascorbic acid, also known as vitamin C, is a vital antioxidant widely found in plants. Plant fruits are rich in ascorbic acid and are the primary source of human intake of ascorbic acid. Ascorbic acid affects fruit ripening and stress resistance and plays an essential regulatory role in fruit development and postharvest storage. The ascorbic acid metabolic pathway in plants has been extensively studied. Ascorbic acid accumulation in fruits can be effectively regulated by genetic engineering technology. The accumulation of ascorbic acid in fruits is regulated by transcription factors, protein interactions, phytohormones, and environmental factors, but the research on the regulatory mechanism is still relatively weak. This paper systematically reviews the regulation mechanism of ascorbic acid metabolism in fruits in recent decades. It provides a rich theoretical basis for an in-depth study of the critical role of ascorbic acid in fruits and the cultivation of fruits rich in ascorbic acid.

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Section: Regeneration and Degradation Of Ascorbic Acidmentioning

confidence: 99%

Metabolism and Regulation of Ascorbic Acid in Fruits

Zheng

Gong

Zhang

et al. 2022

Plants

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“…The most important nutritional quality of Brassicaceae is the content of AsA, which can be regenerated from monodehydroascorbate and dehydroascorbate by MONODEHYDROASCORBATE REDUCTASE ( MDHAR ). Overexpression of MDHAR1 from non-heading Chinese cabbage reduces the AsA level and growth in transgenic tobacco ( Ren et al, 2015 ). Additionally, there is a very significant negative correlation between the nitrate content and AsA content in leaf lettuce ( Koyama et al, 2012 ).…”

Section: Quality Characteristics Of Brassicaceaementioning

confidence: 99%

“…targets of ARF2, CARBON-METABOLISM INVOLVED (GNC) and GNC-LIKE (GNL) are involved in plant greening, flowering time, and senescence (Richter et al, 2013). It has been reported that the constitutive activation of GA signaling is sufficient to suppress arf2 mutant phenotypes, causing increased chlorophyll accumulation and delayed senescence through repression of GNC and GNL (Richter et al, 2013). These and other aspects of different pathways suggest complex crosstalk in multiple phytohormone signals, reflecting their pleiotropic effects on vegetable growth and ripening.…”

Section: Complexity Of Vegetable Quality Regulationmentioning

confidence: 99%

Advances in Understanding and Harnessing the Molecular Regulatory Mechanisms of Vegetable Quality

Gao

Hao

et al. 2022

Front. Plant Sci.

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The quality of vegetables is facing new demands in terms of diversity and nutritional health. Given the improvements in living standards and the quality of consumed products, consumers are looking for vegetable products that maintain their nutrition, taste, and visual qualities. These requirements are directing scientists to focus on vegetable quality in breeding research. Thus, in recent years, research on vegetable quality has been widely carried out, and many applications have been developed via gene manipulation. In general, vegetable quality traits can be divided into three parts. First, commodity quality, which is most related to the commerciality of plants, refers to the appearance of the product. The second is flavor quality, which usually represents the texture and flavor of vegetables. Third, nutritional quality mainly refers to the contents of nutrients and health ingredients such as soluble solids (sugar), vitamin C, and minerals needed by humans. With biotechnological development, researchers can use gene manipulation technologies, such as molecular markers, transgenes and gene editing to improve the quality of vegetables. This review attempts to summarize recent studies on major vegetable crops species, with Brassicaceae, Solanaceae, and Cucurbitaceae as examples, to analyze the present situation of vegetable quality with the development of modern agriculture.

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“…However, there is still some controversy about the role of MDHAR in regulating AsA accumulation in fruits. For example, overexpression of the BcMDHAR gene from cabbage in tobacco led to a decrease in AsA content [19], while overexpression of the MDHAR gene from cherry in tobacco resulted in higher levels of AsA content [20]. The functionally deficient mutant of the MDHAR4 gene in Arabidopsis thaliana had a decreased ratio of ascorbic acid to dehydroascorbate but did not affect the content of total AsA [21].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Comparative Transcriptome Methods Reveal FaMDHAR50 Regulating Ascorbic Acid Regeneration and Quality Formation of Strawberry Fruits

Hou

Yang

et al. 2023

IJMS

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Ascorbic acid (AsA) is a crucial water-soluble antioxidant in strawberry fruit, but limited research is currently available on the identification and functional validation of key genes involved in AsA metabolism in strawberries. This study analyzed the FaMDHAR gene family identification, which includes 168 genes. Most of the products of these genes are predicted to exist in the chloroplast and cytoplasm. The promoter region is rich in cis-acting elements related to plant growth and development, stress and light response. Meanwhile, the key gene FaMDHAR50 that positively regulates AsA regeneration was identified through comparative transcriptome analysis of ‘Benihoppe’ strawberry (WT) and its natural mutant (MT) with high AsA content (83 mg/100 g FW). The transient overexpression experiment further showed that overexpression of FaMDHAR50 significantly enhanced the AsA content by 38% in strawberry fruit, with the upregulated expression of structural genes involved in AsA biosynthesis (FaGalUR and FaGalLDH) and recycling and degradation (FaAPX, FaAO and FaDHAR) compared with that of the control. Moreover, increased sugar (sucrose, glucose and fructose) contents and decreased firmness and citric acid contents were observed in the overexpressed fruit, which were accompanied by the upregulation of FaSNS, FaSPS, FaCEL1 and FaACL, as well as the downregulation of FaCS. Additionally, the content of pelargonidin 3-glucoside markedly decreased, while cyanidin chloride increased significantly. In summary, FaMDHAR50 is a key positive regulatory gene involved in AsA regeneration in strawberry fruit, which also plays an important role in the formation of fruit flavor, apperance and texture during strawberry fruit ripening.

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Overexpression of the Monodehydroascorbate Reductase Gene from Non-heading Chinese Cabbage Reduces Ascorbate Level and Growth in Transgenic Tobacco

Cited by 11 publications

References 44 publications

Metabolism and Regulation of Ascorbic Acid in Fruits

Metabolism and Regulation of Ascorbic Acid in Fruits

Advances in Understanding and Harnessing the Molecular Regulatory Mechanisms of Vegetable Quality

Genome-Wide Identification and Comparative Transcriptome Methods Reveal FaMDHAR50 Regulating Ascorbic Acid Regeneration and Quality Formation of Strawberry Fruits

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