Overexpression of the peanut CLAVATA1-like leucine-rich repeat receptor-like kinase AhRLK1 confers increased resistance to bacterial wilt in tobacco

Abstract

Overexpression of a novel peanut CLAVATA1-like gene significantly enhanced the resistance to Ralstonia solanacearum in tobacco via defense response signaling associated with EDS1-mediated R-gene pathways.

Comparative transcriptome analysis revealed molecular mechanisms of peanut leaves responding to Ralstonia solanacearum and its type III secretion system mutant

“…Previously, by microarray analysis, three BW resistance-related genes AhRLK1 , AhRRS5, and AhGLKb were identified in peanut ( Zhang et al, 2017 , 2019 ; Ali et al, 2020 ). To determine the sampling timepoints which are very important for transcriptome analysis, we analyzed the expression of these three genes in the leaves of A165 and A281 at 0, 6, 12, 24, 36, 48, 72, and 96 hpi with HA4-1 through quantitative real-time PCR (qRT-PCR).…”

“…Leaf inoculation, which bypass the root, can avoid infection randomness and is conducive to accurate study ( Xue et al, 2020 ). In peanut and tobacco, some important genes response to BW were identified by the expression analysis of leaves with RSI, and the functions of these genes were verified later ( Kiba et al, 2007 ; Maimbo et al, 2010 ; Makoto et al, 2015 ; Zhang et al, 2017 , 2019 ; Ali et al, 2020 ). RNA-seq analysis of peanut roots with RSI had been performed in a previous study ( Chen et al, 2014 ).…”

“…There was only the above one report about the transcriptome changes analysis of peanut roots with RSI at present, and transcriptome analysis of peanut leaves with RSI was lacking. Peanut BW resistance-related genes AhRLK1 , AhRRS5 , and AhGLK1b were identified through microarray analysis ( Zhang et al, 2017 , 2019 ; Ali et al, 2020 ). The overexpression of the three genes in tobacco enhanced BW resistance of the transgenic lines.…”

QTL dissection and mining of candidate genes for Ascochyta fabae and Orobanche crenata resistance in faba bean (Vicia faba L.)

“…Medtr8g095030 encodes a leucine-rich repeat receptor-like serine/threonine-protein kinase (LRR-RLK), which are part of the pattern recognition receptors (PRRs) on the plant cell surface that recognize pathogen-associated molecular patterns (PAMPs) to elicit the first-layer immune response [ 27 , 28 ]. LRR-RLKs protein kinases regulate plant growth and development, hormone signal transduction and response to biotic or abiotic stresses [ 29 ]. On the other hand, Medtr4g087620 is annotated as a homolog of the mitogen-activated protein kinase (MAPK) MMK1.…”

Complete Genome Sequence Analysis of Ralstonia solanacearum Strain PeaFJ1 Provides Insights Into Its Strong Virulence in Peanut Plants

“…Although bacterial wilt has been well studied in other plants, there are few studies in peanut, and current research mainly focused on transcriptome change after R. solanacearum inoculation and resistance marker screening ( Chen et al, 2014 ; Luo et al, 2019 ). Up to now, some quantitative trait locus (QTLs) and resistance genes (e.g., AhRLK1 , AhRRS5 , and AhGLKb ) against bacterial wilt have been identified in peanut ( Zhang et al, 2017 , 2019 ; Ali et al, 2020 ), but the molecular mechanism of peanut– R. solanacearum interaction is still poorly understood. The main reasons for this are that the peanut plant itself is not easy to conduct molecular experiments with, and it is the lack of specialized and in-depth research on the pathogenic bacteria causing peanut bacterial wilt.…”