Overexpression of the Prunus sogdiana NBS-LRR Subgroup Gene PsoRPM2 Promotes Resistance to the Root-Knot Nematode Meloidogyne incognita in Tobacco

Abstract

Root-knot nematodes (RKNs), particularly Meloidogyne incognita, are the most devastating soil-borne pathogens that significantly affect the production of Prunus spp. fruit. RKN infection is difficult to control and consequently causes massive yield losses each year. However, several germplasms of wild Prunus spp. have been shown to display resistance to M. incognita. Consequently, both the isolation of novel plant resistance (R) genes and the characterization of their resistance mechanisms are important strategies for future disease control. R proteins require the co-chaperone protein HSP90-SGT1-RAR1 to achieve correct folding, maturation, and stabilization. Here, we used homologous cloning to isolate the R gene PsoRPM2 from the RKN-resistant species Prunus sogdiana. PsoRPM2 was found to encode a TIR-NB-LRR-type protein and react with significantly elevated PsoRPM2 expression levels in response to RKN infection. Transient expression assays indicated PsoRPM2 to be located in both the cytoplasm and the nucleus. Four transgenic tobacco lines that heterologously expressed PsoRPM2 showed enhanced resistance to M. incognita. Yeast two-hybrid analysis and bimolecular fluorescence complementation analysis demonstrated that both PsoRAR1 and PsoRPM2 interacted with PsoHSP90-1 and PsoSGT1, but not with one another. These results indicate that the observed PsoRPM2-mediated RKN resistance requires both PsoHSP90-1 and PsoSGT1, further suggesting that PsoRAR1 plays a functionally redundant role in the HSP90-SGT1-RAR1 co-chaperone.

TaRPM1 Positively Regulates Wheat High-Temperature Seedling-Plant Resistance to Puccinia striiformis f. sp. tritici

“…As intracellular receptors, NBS-LRR proteins could bind pathogen effectors directly or indirectly through perceiving effector-induced modifications to other proteins, inducing host defense responses (Eitas and Dangl, 2010;Li et al, 2015). To date, numerous NBS-LRR genes have been reported to be involved in resistance to pathogens (Axtell and Staskawicz, 2003;Mackey et al, 2003;Shao et al, 2003;Cherkis et al, 2012;Jun et al, 2016;Macqueen et al, 2016;Zhu et al, 2017). However, functions of NBS-LRR genes in the wheat HTSP to Pst remain unknown.…”

Hsp90 Interacts With Tm-22 and Is Essential for Tm-22-Mediated Resistance to Tobacco mosaic virus

“…Using high throughput VIGS assay, Hsp90 was characterized to be a cofactor of Rx protein to stabilize its protein level ( Lu et al, 2003 ). Association of Hsp90 with NBS-LRR proteins has been reported ( Hubert et al, 2003 ; Liu et al, 2004 ; Zhu et al, 2017 ). Hsp90 associates with N protein directly to modulate the immune response to TMV ( Liu et al, 2004 ).…”

“…Hsp90, SGT1 and Rar1 form a complex and act as co-chaperones during virus disease resistance ( Picard, 2002 ). In fact, the structurally conserved Hsp90-SGT1 complex ( Seo et al, 2008 ; Shirasu, 2009 ) are functionally required for different NBS-LRR proteins’ function as immune modulator against various pathogens including bacteria ( Takahashi et al, 2003 ; Zhang et al, 2004 ), fungi ( Bieri et al, 2004 ; Thao et al, 2007 ), oomycetes ( Michael Weaver et al, 2006 ; Bhaskar et al, 2008 ; Oh et al, 2014 ), nematodes ( Bhattarai et al, 2007 ; Zhu et al, 2017 ). Here we reported that Hsp90 directly interacts with Tm-2 2 , a CC-NBS-LRR type of resistance protein, confers robust immune response against tobamoviruses.…”

Genome-Wide Association Study for Resistance to the <em>Meloidogyne javanica</em> Causing Root-Knot Nematode in Soybean

“…Silencing the HSP90 in tomato, increased the number of eggs and galls in tomato plants exposed to M. javanica [61]. As well as, it was demonstrated that the PsoRPM2 gene coding a TIR-NB-LRR-type protein in Tobacco has high expression levels in presence of M. incognita, it was also observed that PsoRPM2 gene interacted with the HSP90 protein [62]. Furthermore, several Hsp20 proteins in soybean were reported as differentially expressed in PI 595099 and cv.…”