Overexpression of the RieskeFeS Protein Increases Electron Transport Rates and Biomass Yield

Simkin, Andrew J.; McAusland, Lorna; Lawson, Tracy; Raines, Christine A.

doi:10.1104/pp.17.00622

Cited by 139 publications

(116 citation statements)

References 81 publications

(107 reference statements)

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“…There is an increasing amount of evidence that the amount of Rieske FeS protein, one of the two nuclear-coded subunits along with PetM, regulates the abundance of cytb 6 f 6, [19][20][21][22][23][24] . Transgenic Arabidopsis thaliana plants overexpressing Rieske FeS also showed an increase in the amounts of other cytb 6 f subunits, positive effects PSII electron transport rate and CO 2 assimilation rate and decreased NPQ 6 . Studies on transgenic tobacco plants indicate that cytb 6 f determines the rate of electron transport through the electron transport chain and concomitantly the CO 2 assimilation rate [19][20][21][22][23] .…”

Section: Introductionmentioning

confidence: 97%

“…Electron transfer reactions of photosynthesis (also called light reactions) supply ATP and NADPH essential for CO 2 assimilation and therefore are a target for improvement 4 . It has been demonstrated in C 3 plants that facilitating electron transport by overexpressing the components of electron transfer chain can result in higher assimilation rates 5,6 . As C 4 plants play a key role in world agriculture with maize and sorghum being major contributors to world food production and sugarcane, miscanthus and switchgrass being major plant sources of bioenergy 7 , improvement of electron transport reactions could further increase the rates of C 4 photosynthesis and yield 8,9 . In C 3 plants the electron transport chain is localised to the thylakoids membranes of mesophyll cells and consists of four major protein complexes: Photosystem II (PSII),…”

Section: Introductionmentioning

confidence: 99%

“…Cytb 6 f oxidises plastoquinol reduced by PSII and reduces plastocyanin, which then diffuses to PSI; plastoquinol oxidation is a rate-limiting step in the intersystem chain 10 . As a result of Q-cycle operating between the two binding sites of cytb 6 f, two protons are translocated from the stroma to the lumen per one electron 11 . Once pools of inorganic phosphate are exhausted, ATP synthesis slows down and causes a build-up of the pmf across the membrane 12 .…”

Section: Introductionmentioning

confidence: 99%

“…Cytb 6 f forms a homodimer where each monomer consists of eight subunits: major subunits, Rieske FeS protein (PetC), cyt b 6 (PetB), cyt f (PetA) and subunit IV (PetD), and minor subunits, PetG, PetL, PetM and PetN 18 . There is an increasing amount of evidence that the amount of Rieske FeS protein, one of the two nuclear-coded subunits along with PetM, regulates the abundance of cytb 6 f 6, [19][20][21][22][23][24] .…”

Section: Introductionmentioning

confidence: 99%

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Overexpression of the Rieske FeS protein of the Cytochromeb₆fcomplex increases C₄photosynthesis

Ermakova

Raines

et al. 2019

Preprint

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C 4 plants contribute 20% to the global primary productivity despite representing only 4% of higher plant species. Their CO 2 concentrating mechanism operating between mesophyll and bundle sheath cells increases CO 2 partial pressure at the site of Rubisco and hence photosynthetic efficiency. Electron transport chains in both cell types supply ATP and NADPH for C 4 photosynthesis. Since Cytochrome b 6 f is a key point of control of electron transport in C 3 plants, we constitutively overexpressed the Rieske FeS subunit in Setaria viridis to study the effects on C 4 photosynthesis. Rieske FeS overexpression resulted in a higher content of Cytochrome b 6 f in both mesophyll and bundle sheath cells without marked changes in abundances of other photosynthetic complexes and Rubisco. Plants with higher Cytochrome b 6 f abundance showed better light conversion efficiency in both Photosystems and could generate higher proton-motive force across the thylakoid membrane. Rieske FeS abundance correlated with CO 2 assimilation rate and plants with a 10% increase in Rieske FeS content showed a 10% increase in CO 2 assimilation rate at ambient and saturating CO 2 and high light. Our results demonstrate that Cytochrome b 6 f controls the rate of electron transport in C 4 plants and that removing electron transport limitations can increase the rate of C 4 photosynthesis.

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Overexpression of the Rieske FeS protein of the Cytochromeb₆fcomplex increases C₄photosynthesis

Ermakova

Raines

et al. 2019

Preprint

Self Cite

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show abstract

“…Over the past decade, several studies have manipulated enzymes to bolster photosynthesis and increase the acquisition of biomass (Long et al, 2015). One such study in this issue found that overexpression of the RieskeFeS protein, a component of the cytochrome b 6 f complex that connects the two photosystem reaction centers, significantly increased PSI and PSII electron transport efficiency, CO 2 assimilation, and yield in Arabidopsis (Simkin et al, 2017).…”

Section: Photosynthesis: Maximizing Flexibility and Outputmentioning

confidence: 99%

The Dynamic Plant: Capture, Transformation, and Management of Energy

Bailey‐Serres¹,

Pierik²,

Ruban³

et al. 2018

Plant Physiology

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Plants are exquisite in their capacity to convert photons of light through photosynthetic carbon dioxide (CO 2 ) fixation into carbohydrate resources that are assimilated and partitioned from photosynthetic source to sink tissues. The chemical energy gained from photosynthesis includes ATP and NADPH that along with sugars are vital to biosynthetic processes, cell proliferation, biomass production, and reproductive fitness. As in all eukaryotes, plants are dependent upon dioxygen (O 2 ) for efficient production of ATP through aerobic respiration by mitochondria. Therefore, O 2 is crucial for the efficient catabolism of carbohydrates, lipids, and protein into chemical energy in leaves in the light and darkness, as well as in sink tissues. In this Focus Issue, numerous reviews and research articles explore the integration of light, O 2 , and energy metabolism from the cellular to the whole-plant level. The articles analyze molecular, biochemical, physiological, and developmental mechanisms that contribute to the plant's energy balance. A recurrent theme is the integration of light, O 2 , and sugar sensing with signal transduction and gene regulation, resulting in metabolic and developmental plasticity that maximizes available energy for growth. This knowledge expands opportunities to enhance photosynthetic efficiency and finetune energy allocation to maximize yields of crops.

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Modelling the impact of improved photosynthetic properties on crop performance in Europe

Harbinson

Yin

2022

Food and Energy Security

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Using the GECROS model, we simulated the effect of improvements in photosynthesis a range of growth parameters, including yield, and on the εc (the conversion efficiency of absorbed solar energy to the chemical energy of biomass) and εi (the efficiency of solar energy interception or absorption by the canopy) parameters of the Monteith crop growth equation, for wheat and potato (which use C3 photosynthesis) and maize (which uses C4 photosynthesis). In the case of the C3 crops, the improvements in photosynthesis were via 20% increases in the parameters Vcmax (carboxylation capacity of Rubisco), Jmax (electron transport capacity), Sc/o (Rubisco specificity), κ2LL (efficiency of converting incident light into electron transport) and gm (mesophyll conductance), while for the C4 crop, it was via 20% increase in Vcmax, Jmax and Sc/o and a 20% decrease in gbs (the conductance that controls the leak of CO2 from the bundle sheath cells in C4 leaves). The changes were applied individually and in combination. The responses were modelled using climate data collected over a 10‐year period from 66 sites around Europe. Improvements in photosynthesis did result in increases in yield but with considerable variation between the parameters that were adjusted. The greatest increases were obtained for increases in Jmax and κ2LL (up to an average 11% increase for total plant biomass), and these increases were found across all Europe. Increases in both these parameters have a predominant effect on the light‐use efficiency for subsaturating irradiances. Improvements in the other parameters produced smaller increases.

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Overexpression of the RieskeFeS Protein Increases Electron Transport Rates and Biomass Yield

Cited by 139 publications

References 81 publications

Overexpression of the Rieske FeS protein of the Cytochromeb₆fcomplex increases C₄photosynthesis

Overexpression of the Rieske FeS protein of the Cytochromeb₆fcomplex increases C₄photosynthesis

The Dynamic Plant: Capture, Transformation, and Management of Energy

Modelling the impact of improved photosynthetic properties on crop performance in Europe

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Overexpression of the RieskeFeS Protein Increases Electron Transport Rates and Biomass Yield

Cited by 139 publications

References 81 publications

Overexpression of the Rieske FeS protein of the Cytochromeb6fcomplex increases C4photosynthesis

Overexpression of the Rieske FeS protein of the Cytochromeb6fcomplex increases C4photosynthesis

The Dynamic Plant: Capture, Transformation, and Management of Energy

Modelling the impact of improved photosynthetic properties on crop performance in Europe

Contact Info

Product

Resources

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Overexpression of the Rieske FeS protein of the Cytochromeb₆fcomplex increases C₄photosynthesis

Overexpression of the Rieske FeS protein of the Cytochromeb₆fcomplex increases C₄photosynthesis