Overexpression of the RNA binding protein MhYTP1 in transgenic apple enhances drought tolerance and WUE by improving ABA level under drought condition

Guo, Tianli; Wang, Na; Xue, Yangchun; Guan, Qingmei; Nocker, Steve van; Liu, Chenlu; Ma, Fengwang

doi:10.1016/j.plantsci.2018.11.018

Cited by 46 publications

(27 citation statements)

References 84 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When we assessed the whole-plant WUE by the ratio between the plant biomass gain (% of initial area) and water irrigated throughout the experimental period, the OsERF115/AP2EREBP110-OE rice exhibited higher WUE than WT plants (Figure 4f). Previous studies have shown that drought-tolerant plants have lower water loss and higher WUE in water-constrained environments compared to plants that do not [21,[74][75][76][77]. We also showed that the Os-ERF115/AP2EREBP110 OE lines exhibited an enhanced drought tolerance phenotype compared to WT plants by the conventional drought stress assay in a greenhouse (Figure S6).…”

Section: Discussionsupporting

confidence: 71%

The OsERF115/AP2EREBP110 Transcription Factor Is Involved in the Multiple Stress Tolerance to Heat and Drought in Rice Plants

Park

Kwon

Cho

et al. 2021

IJMS

View full text Add to dashboard Cite

The AP2/EREBP family transcription factors play important roles in a wide range of stress tolerance and hormone signaling. In this study, a heat-inducible rice ERF gene was isolated and functionally characterized. The OsERF115/AP2EREBP110 was categorized to Group-IIIc of the rice AP2/EREBP family and strongly induced by heat and drought treatment. The OsERF115/AP2EREBP110 protein targeted to nuclei and suppressed the ABA-induced transcriptional activation of Rab16A promoter in rice protoplasts. Overexpression of OsERF115/AP2EREBP110 enhanced thermotolerance of seeds and vegetative growth stage plants. The OsERF115/AP2EREBP110 overexpressing (OE) plants exhibited higher proline level and increased expression of a proline biosynthesis P5CS1 gene. Phenotyping of water use dynamics of the individual plant indicates that the OsERF115/AP2EREBP110-OE plant exhibited better water saving traits under heat and drought combined stress. Our combined results suggest the potential use of OsERF115/AP2EREBP110 as a candidate gene for genetic engineering approaches to develop heat and drought stress-tolerant crops.

show abstract

Section: Discussionsupporting

confidence: 71%

The OsERF115/AP2EREBP110 Transcription Factor Is Involved in the Multiple Stress Tolerance to Heat and Drought in Rice Plants

Park

Kwon

Cho

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…Consistent with previous results, MhYTP1 overexpression in apples promoted drought tolerance through elevated ABA content, increased stomatal density, reduced stomatal aperture, enhanced the net photosynthesis rate, increased biomass, and increased water use efficiency (WUE) under drought conditions [41]. The cis-regulatory elements of YTH domains and abiotic stress gene expression analyses revealed that YTH proteins might participate in heat, drought, salinity, and cold stresses [18,[38][39][40][41][42]. One of the isoforms of the polyadenylation factor was designated as CPSF30-L, which has YTH domain, and was shown to influence the expression of multiple abiotic stress genes in Arabidopsis [110].…”

Section: Yth Domainsupporting

confidence: 90%

Plant RNA Binding Proteins as Critical Modulators in Drought, High Salinity, Heat, and Cold Stress Responses: An Updated Overview

Muthusamy

Kim

et al. 2021

IJMS

View full text Add to dashboard Cite

Plant abiotic stress responses are tightly regulated by different players at multiple levels. At transcriptional or post-transcriptional levels, several RNA binding proteins (RBPs) regulate stress response genes through RNA metabolism. They are increasingly recognized as critical modulators of a myriad of biological processes, including stress responses. Plant RBPs are heterogeneous with one or more conservative RNA motifs that constitute canonical/novel RNA binding domains (RBDs), which can bind to target RNAs to determine their regulation as per the plant requirements at given environmental conditions. Given its biological significance and possible consideration as a potential tool in genetic manipulation programs to improve key agronomic traits amidst frequent episodes of climate anomalies, studies concerning the identification and functional characterization of RBP candidate genes are steadily mounting. This paper presents a comprehensive overview of canonical and novel RBPs and their functions in major abiotic stresses including drought, heat, salt, and cold stress conditions. To some extent, we also briefly describe the basic motif structure of RBPs that would be useful in forthcoming studies. Additionally, we also collected RBP genes that were modulated by stress, but that lacked functional characterization, providing an impetus to conduct further research.

show abstract

“…A WGS survey led to the identification of 26 putative YTP gene models in apple 138 . Next, MhYTP1 and MhYTP2 , characterized in M. hupehensis , were implicated in drought response after 6 days and differentially expressed in several plant tissues with and without stress 137 and overexpression of this gene enhanced WUE in transgenic plants 139,140 .…”

Section: Water and Nutrient Use Efficiency Genes And Genetics: Absorbmentioning

confidence: 99%

Apple whole genome sequences: recent advances and new prospects

Peace¹,

Bianco²,

Troggio³

et al. 2019

Hortic Res

View full text Add to dashboard Cite

In 2010, a major scientific milestone was achieved for tree fruit crops: publication of the first draft whole genome sequence (WGS) for apple ( Malus domestica ). This WGS, v1.0, was valuable as the initial reference for sequence information, fine mapping, gene discovery, variant discovery, and tool development. A new, high quality apple WGS, GDDH13 v1.1, was released in 2017 and now serves as the reference genome for apple. Over the past decade, these apple WGSs have had an enormous impact on our understanding of apple biological functioning, trait physiology and inheritance, leading to practical applications for improving this highly valued crop. Causal gene identities for phenotypes of fundamental and practical interest can today be discovered much more rapidly. Genome-wide polymorphisms at high genetic resolution are screened efficiently over hundreds to thousands of individuals with new insights into genetic relationships and pedigrees. High-density genetic maps are constructed efficiently and quantitative trait loci for valuable traits are readily associated with positional candidate genes and/or converted into diagnostic tests for breeders. We understand the species, geographical, and genomic origins of domesticated apple more precisely, as well as its relationship to wild relatives. The WGS has turbo-charged application of these classical research steps to crop improvement and drives innovative methods to achieve more durable, environmentally sound, productive, and consumer-desirable apple production. This review includes examples of basic and practical breakthroughs and challenges in using the apple WGSs. Recommendations for “what’s next” focus on necessary upgrades to the genome sequence data pool, as well as for use of the data, to reach new frontiers in genomics-based scientific understanding of apple.

show abstract

Overexpression of the RNA binding protein MhYTP1 in transgenic apple enhances drought tolerance and WUE by improving ABA level under drought condition

Cited by 46 publications

References 84 publications

The OsERF115/AP2EREBP110 Transcription Factor Is Involved in the Multiple Stress Tolerance to Heat and Drought in Rice Plants

The OsERF115/AP2EREBP110 Transcription Factor Is Involved in the Multiple Stress Tolerance to Heat and Drought in Rice Plants

Plant RNA Binding Proteins as Critical Modulators in Drought, High Salinity, Heat, and Cold Stress Responses: An Updated Overview

Apple whole genome sequences: recent advances and new prospects

Contact Info

Product

Resources

About