Overexpression of the Saccharomyces cerevisiae RER2 gene in Trichoderma reesei affects dolichol dependent enzymes and protein glycosylation

Perlińska-Lenart, Urszula; Bańkowska, Renata; Palamarczyk, Grażyna; Kruszewska, Joanna S.

doi:10.1016/j.fgb.2006.01.009

Cited by 17 publications

(20 citation statements)

References 40 publications

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“…Overexpression of the RER2 gene resulting in the increased availability of dolichol phosphate and enhanced protein glycosylation (Perlińska-Lenart et al, 2006) reduces sensitivity of sec59-1 or dpm1-6 glycosylation mutants to caspofungin or amphotericin B. This effect strongly supports a relationship between protein glycosylation and sensitivity to antifungal drugs.…”

Section: Discussionmentioning

confidence: 64%

Defect in dolichol‐dependent glycosylation increases sensitivity of Saccharomyces cerevisiae towards anti‐fungal drugs

Juchimiuk

Pasikowska

Zatorska

et al. 2010

Yeast

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Two temperature-sensitive Saccharomyces cerevisiae mutants, sec59-1 and dpm1-6, impaired, respectively, in dolichol kinase (Sec59p) and dolichyl phosphate mannose (DolPMan) synthase (Dpm1p), have an aberrant cell wall structure and composition. We tested their sensitivity to four classes of antifungal drugs used in clinical practice: 5-fluorocytosine, amphotericin B, caspofungin and itraconasole. The strains were resistant to itraconazole and sensitive to the other drugs used. The minimal inhibitory concentration (MIC) of caspofungin and amphotericin B was two-fold lower for sec59-1 and dpm1-6 than for the respective wild-type strains. The sensitivity of both mutants could be brought back to the wild-type level by a multicopy suppressor of the thermosensitive phenotype, the RER2 gene, encoding cis-prenyltransferase involved in dolichol biosynthesis. Biochemical analysis revealed slight changes of the cell wall composition, different in the mutants as compared to the wild-type in response to the drugs. Our data strongly support a relationship between dolichol phosphate level, protein glycosylation and antifungal sensitivity.

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Section: Discussionmentioning

confidence: 64%

Defect in dolichol‐dependent glycosylation increases sensitivity of Saccharomyces cerevisiae towards anti‐fungal drugs

Juchimiuk

Pasikowska

Zatorska

et al. 2010

Yeast

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“…DPM1 , GFA1 and PMI1 encoding dolichol phosphate mannose synthase, glucosamine‐6‐phosphate synthase and phosphomannose isomerase respectively). This modulation of the metabolic flow may have at least two consequences, (1) to increase levels of activated sugars in order to increase remaining glycosylation reactions (Janik et al ., 2003; Perlinska‐Lenart et al ., 2006) and, more speculatively, to reduce the levels of the osmolyte glycerol, to prevent osmotic pressure of the protoplast against the weakened cell wall of pmt mutants (Prill et al ., 2005). The observed downregulation of the OSM1 gene, important for survival in high osmolarity (Singh and Sherman, 1978), could act in the same sense.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional and physiological adaptation to defective protein‐O‐mannosylation in Candida albicans

Cantero¹,

Lengsfeld²,

Prill³

et al. 2007

Molecular Microbiology

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SummaryFive Pmt isoforms O-mannosylate secretory proteins in Candida albicans. Comparisons of genome-wide transcript patterns of each pmt mutant revealed commonly downregulated genes involved in glycolysis and glycerol production. Increased phosphorylation of the Cek1p-but not the Mkc1p-MAP kinase, as well as increased transcript levels for some stress-related genes were detected in the pmt1 strain but not in the other pmt mutants. The transcriptomal pattern after short-term inhibition of Pmt1p activity confirmed stress responses, but did not indicate an alteration of glycolytic flow. Short-but not long-term adaptation to Pmt1p inhibition required signalling components Cek1p, Mkc1p, Efg1p and Tpk1p. Cna1p (calcineurin) but not its downstream effectors Crz1p and Crz2p was generally essential to allow growth during Pmt1p inhibition; accordingly, cyclosporin A strongly inhibited growth of the pmt1 mutant. The lack of Pmt isoforms influenced transcript levels for the remaining isoforms both positively and negatively, suggesting complex cross-regulation among PMT genes. These results confirm individual functions of Pmt isoforms but suggest a common biphasic adaptation response to Pmt deficiency. While known signalling pathways modulate adaptation for a short-term, long-term adaptation requires calcineurin, adjustments of remaining Pmt activities and of glycolytic flow.

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“…Overexpression of the yeast RER-2 gene in T. reesei resulted in overproduction of dolichols, increased activities of enzymes using dolichyl phosphate as an acceptor of carbohydrate residues, and hyperglycosylation of secretory proteins (493). T. reesei, T. atroviride, and T. virens have only one gene coding for cis-PT (TR_21534, TA_299001, and TV_65319).…”

Section: Synthesis Of Farnesyl Diphosphate (Fpp) (I) Acetoacetyl-coamentioning

confidence: 99%

“…In contrast, the Dpm1 protein from S. cerevisiae itself is functional in Trichoderma. Overexpression of the yeast DPM1 gene in T. reesei resulted in 2-fold-higher activity of DPM synthase and increased O-linked glycosylation abilities (493,586).…”

Section: Glycosylationmentioning

confidence: 99%

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

Schmoll

Dattenböck

Carreras-Villaseñor

et al. 2016

Microbiol Mol Biol Rev

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166

195

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SUMMARYThe genusTrichodermacontains fungi with high relevance for humans, with applications in enzyme production for plant cell wall degradation and use in biocontrol. Here, we provide a broad, comprehensive overview of the genomic content of these species for “hot topic” research aspects, including CAZymes, transport, transcription factors, and development, along with a detailed analysis and annotation of less-studied topics, such as signal transduction, genome integrity, chromatin, photobiology, or lipid, sulfur, and nitrogen metabolism inT. reesei,T. atroviride, andT. virens, and we open up new perspectives to those topics discussed previously. In total, we covered more than 2,000 of the predicted 9,000 to 11,000 genes of eachTrichodermaspecies discussed, which is >20% of the respective gene content. Additionally, we considered available transcriptome data for the annotated genes. Highlights of our analyses include overall carbohydrate cleavage preferences due to the different genomic contents and regulation of the respective genes. We found light regulation of many sulfur metabolic genes. Additionally, a new Golgi 1,2-mannosidase likely involved inN-linked glycosylation was detected, as were indications for the ability ofTrichodermaspp. to generate hybrid galactose-containingN-linked glycans. The genomic inventory of effector proteins revealed numerous compounds unique toTrichoderma, and these warrant further investigation. We found interesting expansions in theTrichodermagenus in several signaling pathways, such as G-protein-coupled receptors, RAS GTPases, and casein kinases. A particularly interesting feature absolutely unique toT. atrovirideis the duplication of the alternative sulfur amino acid synthesis pathway.

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Overexpression of the Saccharomyces cerevisiae RER2 gene in Trichoderma reesei affects dolichol dependent enzymes and protein glycosylation

Cited by 17 publications

References 40 publications

Defect in dolichol‐dependent glycosylation increases sensitivity of Saccharomyces cerevisiae towards anti‐fungal drugs

Defect in dolichol‐dependent glycosylation increases sensitivity of Saccharomyces cerevisiae towards anti‐fungal drugs

Transcriptional and physiological adaptation to defective protein‐O‐mannosylation in Candida albicans

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

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