Overexpression of the sweet potato IbOr gene results in the increased accumulation of carotenoid and confers tolerance to environmental stresses in transgenic potato

IbOr Regulates Photosynthesis under Heat Stress by Stabilizing IbPsbP in Sweetpotato

“…Previously, we identified and characterized the gene encoding the Or ortholog ( IbOr ) from sweetpotato (Kim et al, 2013). Overexpression of IbOr results in accumulation of carotenoids and maintenance of higher PSII efficiency upon exposure to abiotic stress (particularly heat stress) in sweetpotato calli, Arabidopsis , alfalfa, and potato (Kim et al, 2013; Goo et al, 2015; Wang et al, 2015; Park et al, 2016). These results suggest that IbOr might play important roles in protecting photosynthesis against abiotic stress via regulation of carotenoid biosynthesis and stabilization of PSII.…”

Overexpression of the IbOr gene from sweet potato (Ipomea batatas ‘Hoang Long’)in maize increases total carotenoid and β-carotene contents

“…The function of the Or gene is related to the differentiation of chromoplasts and accumulation of carotenoids in most of the plants investigated so far; however, the functional relation between the Or transgene and endogenous carotenoid biosynthesis genes as well as levels of carotenoids in wild-type plants is still not fully understood. Several reports showed that the overexpression of the Or gene can affect the endogenous carotenoid synthesis genes (Bai et al, 2014(Bai et al, , 2016Goo et al, 2015), while other studies showed that the overexpression of the Or gene stimulates accumulation of carotenoids without an effect on endogenous carotenoid synthesis genes (Yuan et al, 2015;Zhou et al, 2015). Recently, Berman et al (2017) showed that the overexpression of the Or gene depends on the level and composition of carotenoid of the wild-type plants.…”

“…This protects the stability of IbPSY protein and leads to carotenoid accumulation. The different expression manners of the Or gene have led to varied phenomena: changing both the content and the composition of carotenoids (Lopez et al, 2008), promoting the accumulation of β-carotene and other carotenoids (Lopez et al, 2008;Li et al, 2012), and increasing the total carotenoid content without affecting the composition of carotenoids (Goo et al, 2015;Berman et al, 2017). In our work, the overexpression of the IbOr gene from yellow-fleshed sweet potato cultivar Hoang Long in transgenic maize plants resulted in the increase of both total carotenoids (1.76-10.36-fold) and β-carotene (2.03-15.11-fold).…”

“…In addition to the role in carotenoid accumulation, a recent study showed that the Or protein of Arabidopsis thaliana interacts directly with PSY protein and functions as a major regulator of active PSY protein abundance in mediating carotenoid biosynthesis . The overexpression of the Or gene from Ipomea batatas (IbOr) in transgenic plants of potato, alfalfa, and sweet potato (Li et al, 2012;Goo et al, 2015;Park et al, 2015;Wang et al, 2015;Cho et al, 2016) has led to increased carotenoid accumulation without affecting carotenoid synthesis. A recent report on the role of the Arabidopsis thaliana Or gene (AtOr) in transgenic white maize and their hybrids (Berman et al, 2017) suggested the potential use of the Or gene to improve the carotenoid content of staple crops including maize.…”

Caracterização morfoagronômica, físico-química e tolerância ao nematoide-das-galhas de genótipos de batata-doce avaliados no Distrito Federal

“…Estes compostos são pigmentos orgânicos encontrados em plantas, nas que atuam como antioxidantes, evitando os danos causados por estresses ambientais, como a alta incidência solar, as elevadas temperaturas, a radiação ultravioleta e a seca (GOO et al, 2014). Também desempenham um papel importante como precursores da vitamina A na dieta humana (BECHOFF et al 2010).…”