Overexpression of the tomato glutamate receptor-like genes SlGLR1.1 and SlGLR3.5 hinders Ca2+ utilization and promotes hypersensitivity to Na+ and K+ stresses

Aouini, Asma; Hernould, Michel; Ariizumi, Tohru; Matsukura, Chiaki; Ezura, Hiroshi; Asamizu, Erika

doi:10.5511/plantbiotechnology.12.0213a

Cited by 4 publications

(4 citation statements)

References 27 publications

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“…To identify genes like habanero pepper glutamate receptors, a BLASTP search was performed using the file of habanero pepper coding sequences obtained from the pepper database (Pepper Genome platform) and downloaded into the BioEdit Sequence Alignment Editor Software version 7.2.5 (See Section 4 ). SlGLRs have been previously characterized structurally [ 4 ] and functionally [ 56 ]. For this reason and taking into account that tomatoes belong to the Solanaceae like habanero peppers, these sequences were used as a query.…”

Section: Resultsmentioning

confidence: 99%

In Silico Analysis of Glutamate Receptors in Capsicum chinense: Structure, Evolution, and Molecular Interactions

León-García,

García-Laynes,

Estrada-Tapia

et al. 2024

Plants

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Plant glutamate receptors (GLRs) are integral membrane proteins that function as non-selective cation channels, involved in the regulation of developmental events crucial in plants. Knowledge of these proteins is restricted to a few species and their true agonists are still unknown in plants. Using tomato SlGLRs, a search was performed in the pepper database to identify GLR sequences in habanero pepper (Capsicum chinense Jacq.). Structural, phylogenetic, and orthology analysis of the CcGLRs, as well as molecular docking and protein interaction networks, were conducted. Seventeen CcGLRs were identified, which contained the characteristic domains of GLR. The variation of conserved residues in the M2 transmembrane domain between members suggests a difference in ion selectivity and/or conduction. Also, new conserved motifs in the ligand-binding regions are reported. Duplication events seem to drive the expansion of the species, and these were located in the evolution by using orthologs. Molecular docking analysis allowed us to identify differences in the agonist binding pocket between CcGLRs, which suggest the existence of different affinities for amino acids. The possible interaction of some CcGLRs with proteins leads to suggesting specific functions for them within the plant. These results offer important functional clues for CcGLR, probably extrapolated to other Solanaceae.

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Section: Resultsmentioning

confidence: 99%

In Silico Analysis of Glutamate Receptors in Capsicum chinense: Structure, Evolution, and Molecular Interactions

León-García,

García-Laynes,

Estrada-Tapia

et al. 2024

Plants

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“…Additionally, this systematic review suggests that expression of ECA3 plays a contributive role in mitigating the occurrence of Ca deficiency disorders [55,60], and that overexpression of GLRs and PME may cause Ca deficiency disorders [56,57]. However, the effects of genes for which few studies on Ca deficiency disorders exist need to be further investigated.…”

Section: Synthesizing Current Knowledge Regarding the Role Of Gene Expression In The Occurrence Of Ca 2+ Deficiency Disordersmentioning

confidence: 94%

“…The authors suggested that Ca 2+ deficiency in transgenic plants is mainly due to reduced utilization of the transported Ca 2+ . Aouini et al [57] created transgenic Arabidopsis plants expressing S. lycopersicum GLR1.1, GLR3.5 (SlGLR1.1 and SlGLR3.5). Although there were no significant differences in the Ca 2+ concentration in the aerial parts of the wild-type and transgenic plants, symptoms of Ca 2+ deficiency (necrosis of the tips and margins of young leaves, dwarf stature, and curled and deformed leaves) were observed in transgenic plants.…”

Section: Effects Of Genes Encoding Ca 2+ Channelsmentioning

confidence: 99%

“…To determine the role of gene expression in the occurrence of Ca deficiency disorders and/or Ca accumulation, 37 articles were reviewed in detail (Tables 1 and 2 and Figures 1 and 2). Twenty-six of the thirty-seven reports showed an association between Ca deficiency and/or Ca accumulation and the expression of Ca 2+ -movement-related genes (Ca 2+ antiporters [27,29,32,35,36,39,[42][43][44][45][46][47][48][49][50]53], calreticulin [30,[51][52][53], Ca 2+ -ATPase [35,36,39,54,55], Ca 2+ channels [55][56][57][58], and PME [35,59]). Of the remaining studies, five studies identified QTLs that described variation in tipburn incidence [25,26,33,34,38].…”

Section: Synthesizing Current Knowledge Regarding the Role Of Gene Expression In The Occurrence Of Ca 2+ Deficiency Disordersmentioning

confidence: 99%

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Identification of the Causative Genes of Calcium Deficiency Disorders in Horticulture Crops: A Systematic Review

Kuronuma

Watanabe

2021

Agriculture

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The occurrence of calcium (Ca2+) deficiency disorders is a severe problem in the production of horticulture crops. Recently, several studies have investigated the role of gene expression in Ca2+ deficiency disorders and/or Ca2+ accumulation, providing an indication of the mechanism of Ca2+ deficiency disorders at the genetic level. To determine the relation between gene expression and the occurrence of Ca2+ deficiency disorders, we conducted a systematic review of the literature using the Preferred Reporting Items for Systematic Reviews and Meta-analyses protocol. In our initial search, we extracted studies investigating the relationships between Ca2+ deficiency disorders (tipburn and blossom-end rot) and gene expression. In our second search, we extracted studies involving functional analyses of the genes associated with Ca2+ deficiency and/or Ca2+ accumulation in plant organs. Thirty-seven articles were extracted from both searches. Studies on Ca2+ movement-related genes (Ca2+ antiporters, calreticulin, Ca2+ pumps, Ca2+ channels, and pectin methylesterases) accounted for the majority of these articles. Particularly, the effects of the expression of CAXs (Ca2+/H+ antiporters) and CRT (calreticulin) on the occurrence of Ca2+ deficiency disorders were demonstrated in studies extracted from both searches. Further research focusing on these genes may reveal the causative genes for Ca deficiency disorders in different horticulture crops. We hope that the knowledge synthesized in this systematic review will contribute to the accumulation of further knowledge and elucidation of the causes of Ca2+ deficiency disorders.

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Insights into Blossom End-Rot Disorder in Tomato (Solanum lycopersicum)

Sethi,

Dhaka,

Bari

2024

Plant Mol Biol Rep

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Overexpression of the tomato glutamate receptor-like genes SlGLR1.1 and SlGLR3.5 hinders Ca2+ utilization and promotes hypersensitivity to Na+ and K+ stresses

Cited by 4 publications

References 27 publications

In Silico Analysis of Glutamate Receptors in Capsicum chinense: Structure, Evolution, and Molecular Interactions

In Silico Analysis of Glutamate Receptors in Capsicum chinense: Structure, Evolution, and Molecular Interactions

Identification of the Causative Genes of Calcium Deficiency Disorders in Horticulture Crops: A Systematic Review

Insights into Blossom End-Rot Disorder in Tomato (Solanum lycopersicum)

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