Overexpression of the transcription factor MdWRKY115 improves drought and osmotic stress tolerance by directly binding to the MdRD22 promoter in apple

Dong, Qinglong; Tian, Yi; Zhang, Xuemei; Duan, Dingyue; Zhang, He; Yang, Kaiyu; Jia, Peng; Luan, Haoan; Guo, Shu; Gao, Qi; Mao, Ke; Ma, Fengwang

doi:10.1016/j.hpj.2023.05.005

Cited by 13 publications

(3 citation statements)

References 56 publications

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“…RpMYB98 does not have self-activating activity and can be used for subsequent yeast two-hybrid experiments. This different may be due to containing the specifical inhibitory domain of a TF [ 56 ], which requires the participation of auxiliary TFs to form a transcription complex (Fig. 10 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analysis and identification of candidate R2R3-MYB genes involved in anthraquinone biosynthesis in Rheum palmatum L.

Zhao,

Yan,

et al. 2024

Chin Med

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Background Rheum palmatum L. has important medicinal value because it contains biologically active anthraquinones. However, the key genes and TFs involved in anthraquinone biosynthesis and regulation in R. palmatum remain unclear. Methods Based on full length transcriptome data, in this study, we screened the differentially expressed genes in the anthraquinone biosynthesis pathway. The R2R3-MYB family genes of R. palmatum were systematically identified based on full-length transcriptome sequencing followed by bioinformatics analyses. The correlation analysis was carried out by using co-expression analysis, protein interaction analysis, and real-time fluorescence quantitative analysis after MeJA treatment. The RpMYB81 and RpMYB98 genes were amplified by RT-PCR, and their subcellular localization and self-activation characteristics were analyzed. Results Comparative transcriptome analysis results revealed a total of 3525 upregulated and 6043 downregulated DEGs in the CK versus MeJA group; 28 DEGs were involved in the anthraquinone pathway. Eleven CHS genes that belonged to the PKS family were differentially expressed and involved in anthraquinone biosynthesis. Twelve differentially expressed MYBs genes were found to be co-expressed and interact with CHS genes. Furthermore, 52 MYB genes were identified as positive regulators of anthraquinone biosynthesis and were further characterized. Three MYB genes including RpMYB81, RpMYB98, and RpMYB100 responded to MeJA treatment in R. palmatum, and the levels of these genes were verified by qRT-PCR. RpMYB81 was related to anthraquinone biosynthesis. RpMYB98 had an interaction with genes in the anthraquinone biosynthesis pathway. RpMYB81 and RpMYB98 were mainly localized in the nucleus. RpMYB81 had self-activation activity, while RpMYB98 had no self-activation activity. Conclusion RpMYB81, RpMYB98, and RpMYB100 were significantly induced by MeJA treatment. RpMYB81 and RpMYB98 are located in the nucleus, and RpMYB81 has transcriptional activity, suggesting that it might be involved in the transcriptional regulation of anthraquinone biosynthesis in R. palmatum.

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Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analysis and identification of candidate R2R3-MYB genes involved in anthraquinone biosynthesis in Rheum palmatum L.

Zhao,

Yan,

et al. 2024

Chin Med

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“…DNA purification and sequencing were performed by Bluescape Scientific Co., Ltd. (Hebei, China). The plant genomic DNA extraction kit (Tiangen Biotechnology) was used to extract genomic DNA from mature leaves of 'Gala' (Dong et al, 2023). Clean DNA beads (Bluescape Scientific Co., Ltd., Hebei, China) were used to purify the gDNA, and a NEXTFLEX Rapid DNA Seq Kit (PerkinElmer, Inc., Austin, TX, USA) was used to construct an affinity-purified DNA library.…”

Section: Dap-seq Sampling and Data Analysismentioning

confidence: 99%

The MdVQ37‐MdWRKY100 complex regulates salicylic acid content and MdRPM1 expression to modulate resistance to Glomerella leaf spot in apples

Dong,

Duan,

Wang

et al. 2024

Plant Biotechnology Journal

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SummaryGlomerella leaf spot (GLS), caused by the fungus Colletotrichum fructicola, is considered one of the most destructive diseases affecting apples. The VQ‐WRKY complex plays a crucial role in the response of plants to biotic stresses. However, our understanding of the defensive role of the VQ‐WRKY complex on woody plants, particularly apples, under biotic stress, remains limited. In this study, we elucidated the molecular mechanisms underlying the defensive role of the apple MdVQ37‐MdWRKY100 module in response to GLS infection. The overexpression of MdWRKY100 enhanced resistance to C. fructicola, whereas MdWRKY100 RNA interference in apple plants reduced resistance to C. fructicola by affecting salicylic acid (SA) content and the expression level of the CC‐NBS‐LRR resistance gene MdRPM1. DAP‐seq, Y1H, EMSA, and RT‐qPCR assays indicated that MdWRKY100 inhibited the expression of MdWRKY17, a positive regulatory factor gene of SA degradation, upregulated the expression of MdPAL1, a key enzyme gene of SA biosynthesis, and promoted MdRPM1 expression by directly binding to their promotors. Transient overexpression and silencing experiments showed that MdPAL1 and MdRPM1 positively regulated GLS resistance in apples. Furthermore, the overexpression of MdVQ37 increased the susceptibility to C. fructicola by reducing the SA content and expression level of MdRPM1. Additionally, MdVQ37 interacted with MdWRKY100, which repressed the transcriptional activity of MdWRKY100. In summary, these results revealed the molecular mechanism through which the apple MdVQ37‐MdWRKY100 module responds to GLS infection by regulating SA content and MdRPM1 expression, providing novel insights into the involvement of the VQ‐WRKY complex in plant pathogen defence responses.

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“…For example, PalWRKY77 negatively regulates the expression of PalRD26 and PalNAC002 to reduce salt stress tolerance in poplar [21]. In apple, MdWRKY115 improved tolerance to drought and salt stress by directly binding to the MdRD22 promoter [22]. The findings of various studies have demonstrated that JrWRKY21 and JrPTI5L interact synergistically to form a protein complex, which specifically binds to the GCCGAC motif on the JrPR5L promoter, thereby inducing its expression and ultimately enhancing the walnut's anti-anthrax capability [23].…”

Section: Introductionmentioning

confidence: 99%

ChaWRKY40 Enhances Drought Tolerance of ‘Dawei’ Hazelnuts by Positively Regulating Proline Synthesis

Zhang,

Chao,

Qiu

et al. 2024

Forests

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Hazelnuts are among the most important nuts worldwide. Drought has severely restricted the development of the hazelnut industry in the wake of global warming and lack of water resources. Δ-1-pyrroline-5-carboxylic acid synthase (P5CS) is closely related to drought stress as the rate-limiting enzyme of proline synthesis. WRKY40 had been proven to be an important transcription factor regulating drought tolerance in several plants. In this study, the hybrid hazelnut ‘Dawei’ exhibiting drought tolerance was used as the test material. Tests for simulated drought stress and ChaWRKY40 overexpression, and the yeast one-hybrid assay were performed. The results showed that the relative water content of leaves gradually decreased, but the proline content, electrolyte leakage, and expression of ChaWRKY40 and ChaP5CS increased with increasing PEG-6000 concentration in the leaves. A transient ChaWRKY40 overexpression trial indicated that overexpression of ChaWRKY40 improved the proline content and the transcription level of ChaP5CS. The Y1H experiment suggested that ChaWRKY40 directly binds to the W-box-acting element (W-box) on the promoter of ChaP5CS. In conclusion, ChaWRKY40 may increase the proline content by positively regulating the expression of the ChaP5CS gene, thereby improving the drought resistance of hazelnuts.

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Overexpression of the transcription factor MdWRKY115 improves drought and osmotic stress tolerance by directly binding to the MdRD22 promoter in apple

Cited by 13 publications

References 56 publications

Comparative transcriptome analysis and identification of candidate R2R3-MYB genes involved in anthraquinone biosynthesis in Rheum palmatum L.

Comparative transcriptome analysis and identification of candidate R2R3-MYB genes involved in anthraquinone biosynthesis in Rheum palmatum L.

The MdVQ37‐MdWRKY100 complex regulates salicylic acid content and MdRPM1 expression to modulate resistance to Glomerella leaf spot in apples

ChaWRKY40 Enhances Drought Tolerance of ‘Dawei’ Hazelnuts by Positively Regulating Proline Synthesis

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