Overexpression of the vascular brassinosteroid receptor BRL3 confers drought resistance without penalizing plant growth

Fàbregas, Norma; Lozano-Elena, Fidel; Blasco‐Escámez, David; Tohge, Takayuki; Martínez‐Andújar, Cristina; Albacete, Alfonso; Osorio, Sonia; Bustamante, Mariana; Riechmann, José Luis; Nomura, T.; Yokota, Takao; Conesa, Ana; Alfocea, Francisco Pérez; Fernie, Alisdair R.; Caño‐Delgado, Ana I.

doi:10.1038/s41467-018-06861-3

Cited by 223 publications

(177 citation statements)

References 71 publications

(100 reference statements)

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“…On the other hand, the growth of BR gain-of-function BRI1P-BRI1-OX plants showed less inhibition in response to drought compared to WT. These phenotypes of BRI1P-BRI1-OX are interesting in light of the recent findings showing that overexpression of the vascular BR receptor BRL3, a homolog of BRI1, allows for increased drought survival without compromising plant growth (Fàbregas et al, 2018;Planas-Riverola et al, 2019). Our findings suggest additional complexity in BR-mediated control of drought responses.…”

Section: Discussionsupporting

confidence: 54%

Robotic Assay for Drought (RoAD): An Automated Phenotyping System for Brassinosteroid and Drought Response

Xiang

Nolan

Bao

et al. 2020

Preprint

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Brassinosteroids (BRs) are a group of plant steroid hormones involved in regulating growth, development, and stress responses. Many components of the BR pathway have previously been identified and characterized. However, BR phenotyping experiments are typically performed on petri plates and/or in a lowthroughput manner. Additionally, the BR pathway has extensive crosstalk with drought responses, but drought experiments are time-consuming and difficult to control. Thus, we developed Robotic Assay for Drought (RoAD) to perform BR and drought response experiments in soil-grown Arabidopsis plants. RoAD is equipped with a bench scale, a precisely controlled watering system, an RGB camera, and a laser profilometer. It performs daily weighing, watering, and imaging tasks and is capable of administering BR response assays by watering plants with Propiconazole (PCZ), a BR biosynthesis inhibitor. We developed image processing algorithms for both plant segmentation and phenotypic trait extraction in order to accurately measure traits in 2-dimensional (2D) and 3-dimensional (3D) spaces including plant surface area, leaf length, and leaf width. We then applied machine learning algorithms that utilized the extracted phenotypic parameters to identify image-derived traits that can distinguish control, drought, and PCZ-treated plants. We carried out PCZ and drought experiments on a set of BR mutants and Arabidopsis accessions with altered BR responses. Finally, we extended the RoAD assays to perform BR response assays using PCZ in Zea mays (maize) plants. This study establishes an automated and non-invasive robotic imaging system as a tool to accurately measure morphological and growth-related traits of Arabidopsis and maize plants, providing insights into the BRmediated control of plant growth and stress responses. Arabidopsis thaliana | Zea mays | brassinosteroid | drought | leaf segmentation | phenotypic traits | plant growth | 2D and 3D imaging

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Section: Discussionsupporting

confidence: 54%

Robotic Assay for Drought (RoAD): An Automated Phenotyping System for Brassinosteroid and Drought Response

Xiang

Nolan

Bao

et al. 2020

Preprint

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“…Correlation of root length measurements using MyROOT (y axis) and ImageJ (x axis). Each point corresponds to a different experiment ( n > 20 in each one): time course data from 3 DAG to 8 DAG seedlings (grey) and BR‐related mutants in control and osmotic stress conditions (black, Fabregas et al ., ). Errors bars indicate the standard error.…”

Section: Resultsmentioning

confidence: 97%

“…First, 6‐days‐old seedlings of wild type and BR‐related mutants grown in control and in osmotic stress conditions (data published in Fabregas et al . (), n > 600, Figure ), and second, the same seedlings over 6 consecutive days (from three to eight DAG; n > 116). We obtained a positive correlation between the measurements with both methods ( R 2 = 0.997, Pearson's r = 0.9985).…”

Section: Resultsmentioning

confidence: 97%

“…As a proof of concept, MyROOT software was used for root length measurement of Arabidopsis wild‐type and brassinosteroid (BR)‐signaling mutants grown under control conditions, exogenous BR hormones treatment and for plants grown under osmotic stress conditions (Fabregas et al ., ). MyROOT software is available at https://www.cragenomica.es/research-groups/brassinosteroid-signaling-in-plant-development.…”

Section: Introductionmentioning

confidence: 97%

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MyROOT: a method and software for the semiautomatic measurement of primary root length in Arabidopsis seedlings

et al. 2019

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Summary Root analysis is essential for both academic and agricultural research. Despite the great advances in root phenotyping and imaging, calculating root length is still performed manually and involves considerable amounts of labor and time. To overcome these limitations, we developed MyROOT, a software for the semiautomatic quantification of root growth of seedlings growing directly on agar plates. Our method automatically determines the scale from the image of the plate, and subsequently measures the root length of the individual plants. To this aim, MyROOT combines a bottom‐up root tracking approach with a hypocotyl detection algorithm. At the same time as providing accurate root measurements, MyROOT also significantly minimizes the user intervention required during the process. Using Arabidopsis, we tested MyROOT with seedlings from different growth stages and experimental conditions. When comparing the data obtained from this software with that of manual root measurements, we found a high correlation between both methods (R2 = 0.997). When compared with previous developed software with similar features (BRAT and EZ‐Rhizo), MyROOT offered an improved accuracy for root length measurements. Therefore, MyROOT will be of great use to the plant science community by permitting high‐throughput root length measurements while saving both labor and time.

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“…Moreover, global climate change threatens greater environmental deterioration and risks the continued sustainability of agriculture [4,5]. Plants have evolved many survival strategies to respond to various adverse environmental conditions, including morphological changes, physiological, biochemical, and molecular responses, including global reprogramming of transcription [2,[6][7][8][9][10][11]. Various phytohormones, such as abscisic acid (ABA), brassinosteroids (BRs), jasmonic acid (JA), salicylic acid (SA), ethylene, and cytokinins (CKs), integrate the signaling needed to cooperatively optimize both plant development and the adaptive responses to environmental stressors [12][13][14][15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Comparing and Contrasting the Multiple Roles of Butenolide Plant Growth Regulators: Strigolactones and Karrikins in Plant Development and Adaptation to Abiotic Stresses

Tao

Lian

Wang

2019

IJMS

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Strigolactones (SLs) and karrikins (KARs) are both butenolide molecules that play essential roles in plant growth and development. SLs are phytohormones, with SLs having known functions within the plant they are produced in, while KARs are found in smoke emitted from burning plant matter and affect seeds and seedlings in areas of wildfire. It has been suggested that SL and KAR signaling may share similar mechanisms. The α/β hydrolases DWARF14 (D14) and KARRIKIN INSENSITIVE 2 (KAI2), which act as receptors of SL and KAR, respectively, both interact with the F-box protein MORE AXILLARY GROWTH 2 (MAX2) in order to target SUPPRESSOR OF MAX2 1 (SMAX1)-LIKE/D53 family members for degradation via the 26S proteasome. Recent reports suggest that SLs and/or KARs are also involved in regulating plant responses and adaptation to various abiotic stresses, particularly nutrient deficiency, drought, salinity, and chilling. There is also crosstalk with other hormone signaling pathways, including auxin, gibberellic acid (GA), abscisic acid (ABA), cytokinin (CK), and ethylene (ET), under normal and abiotic stress conditions. This review briefly covers the biosynthetic and signaling pathways of SLs and KARs, compares their functions in plant growth and development, and reviews the effects of any crosstalk between SLs or KARs and other plant hormones at various stages of plant development. We also focus on the distinct responses, adaptations, and regulatory mechanisms related to SLs and/or KARs in response to various abiotic stresses. The review closes with discussion on ways to gain additional insights into the SL and KAR pathways and the crosstalk between these related phytohormones.factors through interactions between the phytohormone regulatory networks via the perception and signal transduction originating at various receptors [20][21][22][23].Strigolactones (SLs) were originally isolated from root exudates of cotton and as seed germination stimulants from plants in the Orobanchaceae family that parasitize plant roots (Striga, Phelipanche, and Orobanche spp.) [24][25][26]. SLs normally control seed germination and seedling development [27], shoot branching [28-32], root architecture [33], and leaf senescence [34]. SLs also promote beneficial symbiotic relationships between host plants and mycorrhizal fungi [35,36]. The biosynthesis and signaling of SLs are regulated by various abiotic stress factors [37][38][39][40], including the recently reported SL involvement in responding to nutrient deprivation, drought, chilling and salinity [38,[40][41][42][43][44][45][46][47][48][49][50]. Such studies provide new insights into the novel roles SL signaling plays in the regulation of plant adaptation to adverse environmental conditions [51][52][53][54].Karrikins (KARs) are found in smoke released from the heating or combustion of plant material, after which they can stimulate the germination of dormant seeds [55][56][57][58]. KARs are also involved in the inhibition of hypocotyl elongation and in the promotion of cotyledon expansion and...

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Overexpression of the vascular brassinosteroid receptor BRL3 confers drought resistance without penalizing plant growth

Cited by 223 publications

References 71 publications

Robotic Assay for Drought (RoAD): An Automated Phenotyping System for Brassinosteroid and Drought Response

Robotic Assay for Drought (RoAD): An Automated Phenotyping System for Brassinosteroid and Drought Response

MyROOT: a method and software for the semiautomatic measurement of primary root length in Arabidopsis seedlings

Comparing and Contrasting the Multiple Roles of Butenolide Plant Growth Regulators: Strigolactones and Karrikins in Plant Development and Adaptation to Abiotic Stresses

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