Overexpression of the white clover TrSAMDC1 gene enhanced salt and drought resistance in Arabidopsis thaliana

Jia, Tong; Hou, Jiaqi; Iqbal, Muhammad Zafar; Zhang, Youzhi; Cheng, Bizhen; Feng, Huahao; Li, Zhou; Liu, Lin; Zhou, Jiqiong; Feng, Guangyan; Nie, Gang; Ma, Xiao; Liu, Wei; Peng, Yan

doi:10.1016/j.plaphy.2021.05.018

Cited by 36 publications

(20 citation statements)

References 60 publications

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“…These findings were consistent with the results from the transcriptome, as PI237232 and Tr005 significantly upregulated genes encoding Put and Spd biosynthesis ( NCA , ADC , SAMDC , and SPDS2 ) and PAs transport ( PAT ) but downregulated Spm biosynthesis-related gene SPMS relative to PI251432 and Korla. It has been reported that transgenic plants overexpressing SAMDC , ADC , and SPDS improved salt tolerance in different plant species by increasing endogenous PA content ( He et al, 2008 ; Espasandin et al, 2018 ; Islam et al, 2020 ; Jia et al, 2021 ). NCA is an enzyme involved in the degradation of N-carbamoylputrescine into Put ( Takahashi and Tong, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Differential Responses to Salt Stress in Four White Clover Genotypes Associated With Root Growth, Endogenous Polyamines Metabolism, and Sodium/Potassium Accumulation and Transport

Geng

Tan

et al. 2022

Front. Plant Sci.

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Selection and utilization of salt-tolerant crops are essential strategies for mitigating salinity damage to crop productivity with increasing soil salinization worldwide. This study was conducted to identify salt-tolerant white clover (Trifolium repens) genotypes among 37 materials based on a comprehensive evaluation of five physiological parameters, namely, chlorophyll (Chl) content, photochemical efficiency of PS II (Fv/Fm), performance index on an absorption basis (PIABS), and leaf relative water content (RWC), and to further analyze the potential mechanism of salt tolerance associated with changes in growth, photosynthetic performance, endogenous polyamine metabolism, and Na+/K+ uptake and transport. The results showed that significant variations in salt tolerance were identified among 37 genotypes, as PI237292 and Tr005 were the top two genotypes with the highest salt tolerance, and PI251432 and Korla were the most salt-sensitive genotypes compared to other materials. The salt-tolerant PI237292 and Tr005 not only maintained significantly lower EL but also showed significantly better photosynthetic performance, higher leaf RWC, underground dry weight, and the root to shoot ratio than the salt-sensitive PI251432 and Korla under salt stress. Increases in endogenous PAs, putrescine (Put), and spermidine (Spd) contents could be key adaptive responses to salt stress in the PI237292 and the Tr005 through upregulating genes encoding Put and Spd biosynthesis (NCA, ADC, SAMDC, and SPDS2). For Na+ and K+ accumulation and transport, higher salt tolerance of the PI237292 could be associated with the maintenance of Na+ and Ca+ homeostasis associated with upregulations of NCLX and BTB/POZ. The K+ homeostasis-related genes (KEA2, HAK25, SKOR, POT2/8/11, TPK3/5, and AKT1/5) are differentially expressed among four genotypes under salt stress. However, the K+ level and K+/Na+ ratio were not completely consistent with the salt tolerance of the four genotypes. The regulatory function of these differentially expressed genes (DEGs) on salt tolerance in the white clover and other leguminous plants needs to be investigated further. The current findings also provide basic genotypes for molecular-based breeding for salt tolerance in white clover species.

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Section: Discussionmentioning

confidence: 99%

Differential Responses to Salt Stress in Four White Clover Genotypes Associated With Root Growth, Endogenous Polyamines Metabolism, and Sodium/Potassium Accumulation and Transport

Geng

Tan

et al. 2022

Front. Plant Sci.

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“…The drought, salt and heat stress treatments were according to Jia et al (2021) [ 60 ]. Briefly, the seeds of homozygous T3 lines of transgenic Arabidopsis (overexpression and T-DNA lines) were used for studying stress responses.…”

Section: Methodsmentioning

confidence: 99%

“…Chlorophyll fluorescence parameters were measured according to Jia et al (2021) [ 60 ]. Briefly, for determining chlorophyll fluorescence parameters, we placed the soil-grown WT and transgenic lines in the dark for 30 min, and measured the maximum quantum yield of PSII photochemistry (Fv/Fm) and PI ABS by a pulse-amplitude modulation portable chlorophyll fluorometer (PAM-2500), using 15 replicates for each treatment.…”

Section: Methodsmentioning

confidence: 99%

A Heat Shock Transcription Factor TrHSFB2a of White Clover Negatively Regulates Drought, Heat and Salt Stress Tolerance in Transgenic Arabidopsis

Iqbal

Jia

Tang

et al. 2022

IJMS

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Heat shock transcription factors (HSF) are divided into classes A, B and C. Class A transcription factors are generally recognized as transcriptional activators, while functional characterization of class B and C heat shock transcription factors have not been fully developed in most plant species. We isolated and characterized a novel HSF transcription factor gene, TrHSFB2a (a class B HSF) gene, from the drought stress-sensitive forage crop species, white clover (Trifolium repens). TrHSFB2a was highly homologous to MtHSFB2b, CarHSFB2a, AtHSFB2b and AtHSFB2a. The expression of TrHSFB2a was strongly induced by drought (PEG6000 15% w/v), high temperature (35 °C) and salt stresses (200 mM L−1 NaCl) in white clover, while subcellular localization analysis showed that it is a nuclear protein. Overexpression of the white clover gene TrHSFB2a in Arabidopsis significantly reduced fresh and dry weight, relative water contents (RWC), maximum photosynthesis efficiency (Fv/Fm) and performance index on the absorption basis (PIABS), while it promoted leaf senescence, relative electrical conductivity (REC) and the contents of malondialdehyde (MDA) compared to a wild type under drought, heat and salt stress conditions of Arabidopsis plants. The silencing of its native homolog (AtHSFB2a) by RNA interference in Arabidopsis thaliana showed opposite trends by significantly increasing fresh and dry weights, RWC, maximum photosynthesis efficiency (Fv/Fm) and performance index on the absorption basis (PIABS) and reducing REC and MDA contents under drought, heat and salt stress conditions compared to wild type Arabidopsis plants. These phenotypic and physiological indicators suggested that the TrHSFB2a of white clover functions as a negative regulator of heat, salt and drought tolerance. The bioinformatics analysis showed that TrHSFB2a contained the core B3 repression domain (BRD) that has been reported as a repressor activator domain in other plant species that might repress the activation of the heat shock-inducible genes required in the stress tolerance process in plants. The present study explores one of the potential causes of drought and heat sensitivity in white clover that can be overcome to some extent by silencing the TrHSFB2a gene in white clover.

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“…The primers used were designed using Primer Premier 5 ( Table S1 ). A stably expressed Trβ-Actin gene was used as the internal control for T. repens ( Table S1 ) [ 34 ]. TrKNOX9 was used as the control gene in five different leaf developmental stages.…”

Section: Methodsmentioning

confidence: 99%

The Identification and Characterization of the KNOX Gene Family as an Active Regulator of Leaf Development in Trifolium repens

Fan

Nie

et al. 2022

Genes

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Leaves are the primary and critical feed for herbivores. They directly determine the yield and quality of legume forage. Trifolium repens (T. repens) is an indispensable legume species, widely cultivated in temperate pastures due to its nutritional value and nitrogen fixation. Although the leaves of T. repens are typical trifoliate, they have unusual patterns to adapt to herbivore feeding. The number of leaflets in T. repens affects its production and utilization. The KNOX gene family encodes transcriptional regulators that are vital in regulating and developing leaves. Identification and characterization of TrKNOX gene family as an active regulator of leaf development in T. repens were studied. A total of 21 TrKNOX genes were identified from the T. repens genome database and classified into three subgroups (Class I, Class II, and Class M) based on phylogenetic analysis. Nineteen of the genes identified had four conserved domains, except for KNOX5 and KNOX9, which belong to Class M. Varying expression levels of TrKNOX genes were observed at different developmental stages and complexities of leaves. KNOX9 was observed to upregulate the leaf complexity of T. repens. Research on TrKNOX genes could be novel and further assist in exploring their functions and cultivating high-quality T. repens varieties.

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Overexpression of the white clover TrSAMDC1 gene enhanced salt and drought resistance in Arabidopsis thaliana

Cited by 36 publications

References 60 publications

Differential Responses to Salt Stress in Four White Clover Genotypes Associated With Root Growth, Endogenous Polyamines Metabolism, and Sodium/Potassium Accumulation and Transport

Differential Responses to Salt Stress in Four White Clover Genotypes Associated With Root Growth, Endogenous Polyamines Metabolism, and Sodium/Potassium Accumulation and Transport

A Heat Shock Transcription Factor TrHSFB2a of White Clover Negatively Regulates Drought, Heat and Salt Stress Tolerance in Transgenic Arabidopsis

The Identification and Characterization of the KNOX Gene Family as an Active Regulator of Leaf Development in Trifolium repens

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