Overexpression of the Wild Soybean R2R3-MYB Transcription Factor GsMYB15 Enhances Resistance to Salt Stress and Helicoverpa Armigera in Transgenic Arabidopsis

Shen, Xinjie; Wang, Yanyan; Zhang, Yongxing; Guo, Wei; Jiao, Yongqing; Zhou, Xinan

doi:10.3390/ijms19123958

Cited by 65 publications

(34 citation statements)

References 66 publications

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“…Moreover, both the promoter of SpPKE1 and SlPKE1 had an MYB and MYC cis -element (Table 1). The MYB and MYC proteins are major transcription factors that play significant roles in plant defenses against various stresses [21,22,23,24,25,26,27,28,29]. These findings, together with our results, show that the MYB or MYC transcription factor may directly regulate PKE1 to confer abiotic stresses.…”

Section: Discussionsupporting

confidence: 77%

SpPKE1, a Multiple Stress-Responsive Gene Confers Salt Tolerance in Tomato and Tobacco

Chen

Juan-juan

et al. 2019

IJMS

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Understanding the mechanism of abiotic-tolerance and producing germplasm of abiotic tolerance are important in plant research. Wild species often show more tolerance of environmental stress factors than their cultivated counterparts. Genes from wild species show potential abilities to improve abiotic resistance in cultivated species. Here, a tomato proline-, lysine-, and glutamic-rich type gene SpPKE1 was isolated from abiotic-resistant species (Solanum pennellii LA0716) for over-expression in tomato and tobacco for salt tolerance. The protein encoded by SpPKE1 was predominantly localized in the cytoplasm in tobacco. SpPKE1 and SlPKE1 (from cultivated species S. lycopersicum cv. M82) shared 89.7% similarity in amino acid sequences and their transcripts abundance in flowers and fruits was reduced by the imposition of drought or oxidative stress and the exogenous supply of abscisic acid. The DNA of the PKE1 promoter was highly methylated in fruit and leaf, and the methylation of the coding sequence in leaf was significantly higher than that in fruit at different development stages. The over-expression of SpPKE1 under the control of a CaMV (Cauliflower Mosaic Virus) 35S promoter in transgenic tomato and tobacco plants enhanced their tolerance to salt stress. PKE1 was downregulated by abiotic stresses but enhanced the plant’s salt stress tolerance. Therefore, this gene may be involved in post-transcriptional regulation and may be an important candidate for molecular breeding of salt-tolerant plants.

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Section: Discussionsupporting

confidence: 77%

SpPKE1, a Multiple Stress-Responsive Gene Confers Salt Tolerance in Tomato and Tobacco

Chen

Juan-juan

et al. 2019

IJMS

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“…The relevant role of the MYB genes during plant stress tolerance has been reported in earlier studies using various transgenic plants. For example, overexpression of the wild soybean R2R3-MYB transcription factor GsMYB15 enhances resistance to salt stress in transgenic A. thaliana (Shen et al, 2018). On the contrary, the R-R-type MYB TF AtDIV2 has a negative role in salt stress, and is required for ABA signaling in A. thaliana (Fang et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Peer Review #1 of "MYB transcription factors in alfalfa (Medicago sativa): genome-wide identification and expression analysis under abiotic stresses (v0.1)"

2019

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Background. Alfalfa is the most widely cultivated forage legume and one of the most economically valuable crops in the world. Its survival and production are often hampered by environmental changes. However, there are few studies on stress-resistance genes in alfalfa because of its incomplete genomic information and rare expression profile data. The MYB proteins are characterized by a highly conserved DNA-binding domain, which is large, functionally diverse, and represented in all eukaryotes. The role of MYB proteins in plant development is essential; they function in diverse biological processes, including stress and defense responses, and seed and floral development. Studies on the MYB gene family have been reported in several species, but they have not been comprehensively analyzed in alfalfa. Methods. To identify more comprehensive MYB transcription factor family genes, the sequences of 168 Arabidopsis thaliana, 430 Glycine max, 185 Medicago truncatula, and 130 Oryza sativa MYB proteins were downloaded from the Plant Transcription Factor Database. These sequences were used as queries in a BLAST search against the Medicago sativa proteome sequences provided by the Noble Research Institute. Results. In the present study, a total of 265 MsMYB proteins were obtained, including 50 R1-MYB, 186 R2R3-MYB, 26 R1R2R3-MYB, and three atypical-MYB proteins. These predicted MsMYB proteins were divided into 12 subgroups by phylogenetic analysis, and gene ontology (GO) analysis indicated that most of the MsMYB genes are involved in various biological processes. The expression profiles and quantitative real-time PCR analysis indicated that some MsMYB genes might play a crucial role in the response to abiotic stresses. Additionally, a total of 170 and 914 predicted protein-protein and protein-DNA interactions were obtained, respectively. The interactions between MsMYB043 and MSAD320162, MsMYB253 and MSAD320162, and MsMYB253 and MSAD308489 were confirmed by a yeast two-hybrid system. This work provides information on the MYB family in alfalfa that was previously lacking and might promotes the cultivation of stress-resistant alfalfa.

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“…AtMYB75 increases resistance against Pieris brassicae by modulating flavonoid metabolites. Overexpression of GsMYB15 obtained from wild soybean increases Arabidopsis resistance against Helicoverpa armigera by a JA mediated insect response [137]. CmMYB19 increases resistance of Chrysanthemum against aphids by promoting a lignin biosynthesis pathway [138].…”

Section: Pest Attackmentioning

confidence: 99%

Harnessing the Potential of Plant Transcription Factors in Developing Climate-Smart Crops: Future Prospects, Challenges, and Opportunities

Shahzad¹,

Jamil²,

Ahmad³

et al. 2020

Preprint

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Crop plants should be resilient to climatic factors in order to feed ever-increasing populations. Plants have developed stress-responsive mechanisms by changing their metabolic pathways and switching the stress-responsive genes. The discovery of plant transcriptional factors (TFs) as key regulators of different biotic and abiotic stresses have opened up new horizons for plant scientists. TFs perceive the signal and switch certain stress-responsive genes on and off by binding to different cis-regulatory elements. The above 50 species of plant TFs have been reported in nature. DREB, bZIP, MYB, NAC, Zinc-finger, HSF, Dof, WRKY, and NF-Y are important with respect to biotic and abiotic stresses whereas the role of many TFs is yet to explore. In this review, we summarize the role of different stress-responsive TFs with respect to biotic and abiotic stresses. Further, challenges and future opportunities linked with TFs for developing climate-resilient crops are also elaborated.

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Overexpression of the Wild Soybean R2R3-MYB Transcription Factor GsMYB15 Enhances Resistance to Salt Stress and Helicoverpa Armigera in Transgenic Arabidopsis

Cited by 65 publications

References 66 publications

SpPKE1, a Multiple Stress-Responsive Gene Confers Salt Tolerance in Tomato and Tobacco

SpPKE1, a Multiple Stress-Responsive Gene Confers Salt Tolerance in Tomato and Tobacco

Peer Review #1 of "MYB transcription factors in alfalfa (Medicago sativa): genome-wide identification and expression analysis under abiotic stresses (v0.1)"

Harnessing the Potential of Plant Transcription Factors in Developing Climate-Smart Crops: Future Prospects, Challenges, and Opportunities

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