Overexpression of tomato chloroplast-targeted DnaJ protein enhances tolerance to drought stress and resistance to Pseudomonas solanacearum in transgenic tobacco

Wang, Guodong; Cai, Guohua; Kong, Fanying; Deng, Yong-Sheng; Ma, Nana; Meng, Qingwei

doi:10.1016/j.plaphy.2014.05.011

Cited by 95 publications

(55 citation statements)

References 41 publications

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“…DnaJ chaperon protein -DnaJ co-chaperons are vital in stress response and has been found to be involved in maintenance of photosystem II under chilling stress and enhances drought tolerance in tomato [93,94].…”

Section: Bradi2g43040mentioning

confidence: 99%

Comparative transcriptomics provides insight into the evolution of cold response in Pooideae

Grønvold

Schubert

Sandve

et al. 2017

Preprint

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Background: Understanding how complex traits evolve through adaptive changes in gene regulation remains a major challenge in evolutionary biology. Over the last ~50 million years, Earth has experienced climate cooling and ancestrally tropical plants have adapted to expanding temperate environments. The grass subfamily Pooideae dominates the grass flora of the temperate regions, but conserved cold-response genes that might have played a role in the cold adaptation to temperate climate remain unidentified.

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Section: Bradi2g43040mentioning

confidence: 99%

Comparative transcriptomics provides insight into the evolution of cold response in Pooideae

Grønvold

Schubert

Sandve

et al. 2017

Preprint

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“…The researchers reported that over-expression of tomato ( Solanum lycopersicum ) chloroplast-targeted J-protein, LeCDJ1 , facilitated heat tolerance in transgenic tomatoes (Kong et al, 2014a) and further found that it also played important role in maintaining photosystem II under chilling stress (Kong et al, 2014b). Subsequently, the study has also demonstrated that this gene could enhance tolerance to drought stress and resistance to P. solanacearum in transgenic tobacco (Wang et al, 2014). In addition, Xia et al (2014) reported that a putative J-proteins ortholog from Nicotiana tabacum could be involved in drought stress response and its over-expression enhanced drought tolerance possibly through regulating expression of stress-responsive genes.…”

Section: Introductionmentioning

confidence: 99%

The DnaJ Gene Family in Pepper (Capsicum annuum L.): Comprehensive Identification, Characterization and Expression Profiles

et al. 2017

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The DnaJ proteins which function as molecular chaperone played critical roles in plant growth and development and response to heat stress (HS) and also called heat shock protein 40 based on molecular weight. However, little was reported on this gene family in pepper. Recently, the release of the whole pepper genome provided an opportunity for identifying putative DnaJ homologous. In this study, a total of 76 putative pepper DnaJ genes (CaDnaJ01 to CaDnaJ76) were identified using bioinformatics methods and classified into five groups by the presence of the complete three domains (J-domain, zinc finger domain, and C-terminal domain). Chromosome mapping suggested that segmental duplication and tandem duplication were occurred in evolution. The multiple stress-related cis-elements were found in the promoter region of these CaDnaJ genes, which indicated that the CaDnaJs might be involved in the process of responding to complex stress conditions. In addition, expression profiles based on RNA-seq showed that the 47 CaDnaJs were expressed in at least one tissue tested. The result implied that they could be involved in the process of pepper growth and development. qRT-PCR analysis found that 80.60% (54/67) CaDnaJs were induced by HS, indicated that they could participated in pepper response to high temperature treatments. In conclusion, all these results would provide a comprehensive basis for further analyzing the function of CaDnaJ members and be also significant for elucidating the evolutionary relationship in pepper.

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“…The transcript levels of several stress‐related genes were analyzed (Wang et al ). NtERD10B , NtERD10C and NtERD10D were up‐regulated in all lines after drought treatment but showed no significant difference between the WT and transgenic plants.…”

Section: Resultsmentioning

confidence: 99%

A novel tomato SUMO E3 ligase, SlSIZ1, confers drought tolerance in transgenic tobacco

Zhang

Zhuang

Wang

et al. 2017

JIPB

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SUMOylation is an important post-translational modification process that regulates different cellular functions in eukaryotes. SIZ/PIAS-type SAP and Miz1 (SIZ1) proteins exhibit SUMO E3 ligase activity, which modulates SUMOylation. However, SIZ1 in tomato has been rarely investigated. In this study, a tomato SIZ1 gene (SlSIZ1) was isolated and its molecular characteristics and role in tolerance to drought stress are described. SlSIZ1 was upregulated by cold, sodium chloride (NaCl), polyethylene glycol (PEG), hydrogen peroxide (H 2 O 2 ) and abscisic acid (ABA), and the corresponding proteins were localized in the nucleus. The expression of SlSIZ1 in Arabidopsis thaliana (Arabidopsis) siz1-2 mutants partially complemented the phenotypes of dwarf, cold sensitivity and ABA hypersensitivity. SlSIZ1 also exhibited the activity of SUMO E3 ligase to promote the accumulation of SUMO conjugates. Under drought stress, the ectopic expression of SlSIZ1 in transgenic tobacco lines enhanced seed germination and reduced the accumulation of reactive oxygen species. SlSIZ1 overexpression conferred the plants with improved growth, high free proline content, minimal malondialdehyde accumulation and increased accumulation of SUMO conjugates. SlSIZ1 is a functional homolog of Arabidopsis SIZ1 with SUMO E3 ligase activity. Therefore, overexpression of SlSIZ1 enhanced the tolerance of transgenic tobacco to drought stress.

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Overexpression of tomato chloroplast-targeted DnaJ protein enhances tolerance to drought stress and resistance to Pseudomonas solanacearum in transgenic tobacco

Cited by 95 publications

References 41 publications

Comparative transcriptomics provides insight into the evolution of cold response in Pooideae

Comparative transcriptomics provides insight into the evolution of cold response in Pooideae

The DnaJ Gene Family in Pepper (Capsicum annuum L.): Comprehensive Identification, Characterization and Expression Profiles

A novel tomato SUMO E3 ligase, SlSIZ1, confers drought tolerance in transgenic tobacco

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