Overexpression of tomato tAPX gene in tobacco improves tolerance to high or low temperature stress

Abstract

In order to investigate the function of chloroplast ascorbate peroxidase under temperature stress, the thylakoid-bound ascorbate peroxidase gene from tomato leaf (TtAPX) was introduced into tobacco. Transformants were selected for their ability to grow on medium containing kanamycin. RNA gel blot analysis confirmed that TtAPX in tomato was induced by chilling or heat stress. Over-expression of TtAPX in tobacco improved seed germination under temperature stress. Two transgenic tobacco lines showed higher ascorbate peroxidase activity, accumulated less hydrogen peroxide and malondialdehyde than wild type plants under stress condition. The photochemical efficiency of photosystem 2 in the transgenic lines was distinctly higher than that of wild type plants under chilling and heat stresses. Results indicated that the over-expression of TtAPX enhanced tolerance to temperature stress in transgenic tobacco plants.Additional key words: Lycopersicum esculentum, malondialdehyde, Nicotiana tabacum, photosystem 2, thylakoid-bound ascorbate peroxidase, transgenic tobacco.

Production of reactive oxygen species in Dalbergia nigra seeds under thermal stress

“…Okane et al (1996) observed that H 2 O 2 production in calluses of Arabidopsis thaliana plants was greater at 4 °C than at 23 °C. Similar results were found by Sun et al (2010), who observed accumulation of H 2 O 2 in leaf tissue under low temperature conditions in Nicotiana tabacum seedlings. According to Torres and Dangl (2005), there was expression of various genes in different species when the plants were subjected to low temperature.…”

Hexanoic acid 2-(diethylamino)ethyl ester enhances chilling tolerance in strawberry seedlings by impact on photosynthesis and antioxidants

“…Reactive oxygen species (ROS) tends to increase in plants exposed to low temperature (Sun et al 2010). Therefore, plants adopt many efficient defense mechanisms to cope with increased rates of ROS.…”

Expression analysis of antioxidant genes in response to drought stress in the fl ag leaf of two Indonesian rice cultivars

“…Other studies reported that transgenic rice containing Cu-ZnSod 1 gene isolated from other species showed better tolerance to drought, salinity or methyl viologen induced-oxidative stress than untransgenic control plants (Wang et al, 2010, Sarangi et al, 2011. Moreover, enhanced oxidative defense in rice and tobacco plants applied to abiotic stress could be achieved through Apx overexpression (Sun et al, 2010;. Overexpression of Cat gene also induced increasing oxidative defense in cabbage plants to reduce ROS damage stimulated by salinity stress (Tseng et al, 2007).…”