Overexpression of TsGOLS2, a galactinol synthase, in Arabidopsis thaliana enhances tolerance to high salinity and osmotic stresses

Sun, Zhibin; Qi, Xiaowen; Wang, Zenglan; Li, Pinghua; Wu, Chunxia; Zhang, Hui; Zhao, Yanxiu

doi:10.1016/j.plaphy.2013.04.009

Cited by 104 publications

(60 citation statements)

References 33 publications

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“…Consistent with these expression patterns, the galactinol content in leaves increased after 4 days of drought stress (Zhou et al 2014). In cucumber plants, the induction of CsRS expression in leaves after 1 h of cold stress and the following increase in CsRS transcripts correlated with an increasing activity of RS and accumulation of raffinose under cold stress (Sun et al 2013).…”

Section: Expression Of Psgols Psrs and Pssts Genes And Activity Of Esupporting

confidence: 60%

“…The accumulation of galactinol and raffinose in vegetative tissues of different plant species occurs under cold, heat, drought and osmotic stresses (ElSayed et al 2014;Pastorczyk et al 2014). The concentrations of accumulated galactinol and/or raffinose can be sufficiently high to confirm the osmoprotective role of both galactosides (Sun et al 2013;Wang et al 2012a;Wu et al 2009). However, in dehydrated seedlings of wheat (Bogdan and Zagdańska 2006), winter vetch (Vicia villosa Roth) (Lahuta and Górecki 2011) and pea , the concentration of accumulated raffinose is very low.…”

Section: Introductionmentioning

confidence: 99%

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Osmotic stress induces genes, enzymes and accumulation of galactinol, raffinose and stachyose in seedlings of pea (Pisum sativum L.)

et al. 2015

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The objective of the present study was to recognize the molecular background of the accumulation of raffinose family oligosaccharides (RFOs) in pea (Pisum sativum L.) seedlings under osmotic stress conditions. The exposure of 5-day-old pea seedlings to osmotic stress for 48 h created by immersing roots in PEG8000 solution (-1.5 MPa) induced synthesis of galactinol and RFOs (raffinose and stachyose) in the epicotyl and root tissues, but not in cotyledons. After 24 h of recovery, galactinol completely disappeared, raffinose decreased fourfold and stachyose decreased twofold in roots, but increased in epicotyls. The temporary accumulation of RFOs resulted from a dramatic increase in the enzymatic activity and changes in expression of galactinol synthase (PsGolS), raffinose synthase (PsRS) and stachyose synthase (PsSTS) genes. PsGolS was induced by osmotic stress in both epicotyls as well as in roots. PsRS and PsSTS were induced only in epicotyls, but repressed or remained unaffected in roots, respectively. During recovery, the expression and activity of PsGolS, PsRS and PsSTS dramatically decreased. The expression of PsGolS gene, that level of mRNA transcript significantly decreased during recovery and whose promoter region was identified to contain some stress-related regulating elements, seems to play a crucial role in the biosynthesis of RFOs under osmotic stress. Possible signals that may trigger the induction of expression of PsGolS, PsRS and PsSTS genes and accumulation of RFOs in pea seedlings are discussed.

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Section: Expression Of Psgols Psrs and Pssts Genes And Activity Of Esupporting

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Osmotic stress induces genes, enzymes and accumulation of galactinol, raffinose and stachyose in seedlings of pea (Pisum sativum L.)

et al. 2015

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“…Similarly to polyamines, proline also acts in various tasks within drought-treated plants, including photosynthesis (Szabados and Savoure, 2010). WSI76 is thought to encode galactinol synthase, which catalyzes the first step in the biosynthesis of raffinose family oligosaccharides (RFO) and has been found out to accumulate in seed and vegetative part of plants after desiccation (Liu et al, 1998; Taji et al, 2002; Li et al, 2011; Sun et al, 2013). Genes encoding WSI76 were shown to reach the highest expression level in leaf tissue of Tad ( Figure 5 , Supplementary Table 1).…”

Section: Discussionmentioning

confidence: 99%

Global Scale Transcriptional Profiling of Two Contrasting Barley Genotypes Exposed to Moderate Drought Conditions: Contribution of Leaves and Crowns to Water Shortage Coping Strategies

et al. 2016

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Drought is a serious threat for sustainable agriculture. Barley represents a species well adapted to environmental stresses including drought. To elucidate the adaptive mechanism of barley on transcriptional level we evaluated transcriptomic changes of two contrasting barley cultivars upon drought using the microarray technique on the level of leaves and crowns. Using bioinformatic tools, differentially expressed genes in treated vs. non-treated plants were identified. Both genotypes revealed tissue dehydration under drought conditions as shown at water saturation deficit and osmotic potential data; however, dehydration was more severe in Amulet than in drought-resistant Tadmor under the same ambient conditions. Performed analysis showed that Amulet enhanced expression of genes related to active plant growth and development, while Tadmor regarding the stimulated genes revealed conservative, water saving strategy. Common reactions of both genotypes and tissues included an induction of genes encoding several stress-responsive signaling proteins, transcription factors as well as effector genes encoding proteins directly involved in stress acclimation. In leaf, tolerant cultivar effectively stimulated mainly the expression of genes encoding proteins and enzymes involved in protein folding, sulfur metabolism, ROS detoxification or lipid biosynthesis and transport. The crown specific reaction of tolerant cultivar was an enhanced expression of genes encoding proteins and enzymes involved in cell wall lignification, ABRE-dependent abscisic acid (ABA) signaling, nucleosome remodeling, along with genes for numerous jasmonate induced proteins.

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“…Increased expression of GolS genes intensifies the accumulation of galactinol and raffinose in tissues and increases plant resistance to stress (Wang et al 2012;Zhuo et al 2013). In a study of transgenic plants with overexpression of the GolS gene, intensified accumulation of galactinol and raffinose increased resistance to cold stress in Arabidopsis (Zuther et al 2004;Sun et al 2013), Photinia serrulata (Song et al 2013) and monocot Brachypodium distachyon (Himuro et al 2014). It remains unknown whether galactinol exerts a protective influence on dehydrated embryonic tissues.…”

Section: Discussionmentioning

confidence: 99%

Soluble carbohydrates in developing and mature diaspores of polar Caryophyllaceae and Poaceae

et al. 2015

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The accumulation of soluble carbohydrates in maturing diaspores of flowering plants comprising Arctic populations of Cerastium alpinum, indigenous Antarctic species Colobanthus quitensis and Deschampsia antarctica, and cosmopolitan Poa annua from the Antarctic was investigated. For comparative purposes, the diaspores of two species of flowering plants growing in the area of Olsztyn (Poland), Poa annua (Poaceae) and Cerastium arvense (Caryophyllaceae) were used. A qualitative and quantitative analysis of soluble carbohydrates conducted by means of high-resolution gas chromatography showed that monosaccharides (glucose and fructose), maltose and sucrose, raffinose, myo-inositol and galactinol are ubiquitous in developing and mature diaspores among investigated species. Moreover, D. antarctica and P. annua caryopses additionally contained stachyose and 1-kestose; the seeds of Caryophyllaceae studied were found to contain D-pinitol and D-ononitol. The development and maturation of the seeds of polar Caryophyllaceae and Poaceae were accompanied by the changes in the concentration of their soluble carbohydrates. During maturation, seeds accumulated galactinol and raffinose family of oligosaccharides (RFOs), except C. quitensis. Although seeds of the studied Caryophyllaceae contained D-pinitol and lower amounts of D-ononitol, they did not accumulate a-D-galactoside derivatives of mentioned cyclitols. P. annua caryopses, occurring in the Antarctic, were found to accumulate considerably higher amounts of sucrose and 1-kestose than those developed in Olsztyn.

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Overexpression of TsGOLS2, a galactinol synthase, in Arabidopsis thaliana enhances tolerance to high salinity and osmotic stresses

Cited by 104 publications

References 33 publications

Osmotic stress induces genes, enzymes and accumulation of galactinol, raffinose and stachyose in seedlings of pea (Pisum sativum L.)

Osmotic stress induces genes, enzymes and accumulation of galactinol, raffinose and stachyose in seedlings of pea (Pisum sativum L.)

Global Scale Transcriptional Profiling of Two Contrasting Barley Genotypes Exposed to Moderate Drought Conditions: Contribution of Leaves and Crowns to Water Shortage Coping Strategies

Soluble carbohydrates in developing and mature diaspores of polar Caryophyllaceae and Poaceae

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