2021
DOI: 10.1007/s10529-021-03103-3
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Overexpression of uracil permease and nucleoside transporter from Bacillus amyloliquefaciens improves cytidine production in Escherichia coli

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Cited by 6 publications
(4 citation statements)
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“…Transporter engineering is one of the common strategies employed in metabolic engineering to enhance the synthesis of compounds in engineered cells. , It has been reported that UraA (uracil/H+ symporter), encoded by the uraA gene, forms a dimer through its interaction with uracil, facilitating the absorption of external uracil by E. coli, and PelB as a signal peptide can enhance the effect of UraA. , Thus, UraA and its coexpression with PelB were overexpressed in strain F4, resulting in strain G1 and strain G2.…”
Section: Resultsmentioning
confidence: 99%
“…Transporter engineering is one of the common strategies employed in metabolic engineering to enhance the synthesis of compounds in engineered cells. , It has been reported that UraA (uracil/H+ symporter), encoded by the uraA gene, forms a dimer through its interaction with uracil, facilitating the absorption of external uracil by E. coli, and PelB as a signal peptide can enhance the effect of UraA. , Thus, UraA and its coexpression with PelB were overexpressed in strain F4, resulting in strain G1 and strain G2.…”
Section: Resultsmentioning
confidence: 99%
“…Excessive accumulation of nucleosides leads to metabolic burden on the cell and causes productmediated feedback inhibition. Previous studies illustrated that the NupG protein of E. coli can transport extracellular nucleosides into cells, and knockout of the nupG and nupC genes can increase the uridine and cytidine titres [11,28,29]. Meanwhile, overexpression of the nucleoside e ux transporters PbuE and NepI notably increased inosine secretion [30].…”
Section: Transporter Engineering For Guanosine Accumulationmentioning
confidence: 99%
“…Excessive accumulation of nucleosides leads to metabolic burden on the cell and causes product-mediated feedback inhibition. Previous studies illustrated that the NupG protein of E. coli can transport extracellular nucleosides into cells, and knockout of the nupG and nupC genes can increase the uridine and cytidine titres [ 11 , 27 , 28 ]. Meanwhile, overexpression of the nucleoside efflux transporters PbuE and NepI notably increased inosine secretion [ 29 ].…”
Section: Blocking the Degradation Pathwaymentioning
confidence: 99%