Overexpression of violaxanthin de-epoxidase gene alleviates photoinhibition of PSII and PSI in tomato during high light and chilling stress

Han, Han; Gao, Shan; Li, Bin; Dong, Xinchun; Feng, Hai-Long; Meng, Qingwei

doi:10.1016/j.jplph.2009.08.009

Cited by 63 publications

(52 citation statements)

References 44 publications

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“…Previous experiments indicated that VDE transcript abundance and protein levels were asynchronous in tomato and Arabidopsis leaves. The transcript abundance of VDE is regulated by the endogenous circadian clock, whereas protein levels remain relatively stable (Han et al 2010;North et al 2005). Pigment analysis confirmed that VDE activities also varied diurnally, but peaks of activity did not correlate with the steady state levels of RNA and protein; the short-term high light (HL) induction of the xanthophyll cycle only seems to involve post-translational regulation.…”

Section: Introductionmentioning

confidence: 98%

“…The VDE precursor proteins are nuclear-encoded, and their corresponding genes have been cloned from a number of species (Han et al 2010;North et al 2005). Several biochemical and molecular genetic studies suggest that the VDE expression pattern is influenced by light and temperature, in particular, by the endogenous circadian clock (Han et al 2010;North et al 2005;Huang et al 2007;Facella et al 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Several biochemical and molecular genetic studies suggest that the VDE expression pattern is influenced by light and temperature, in particular, by the endogenous circadian clock (Han et al 2010;North et al 2005;Huang et al 2007;Facella et al 2008).…”

Section: Introductionmentioning

confidence: 99%

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Molecular cloning and characterization of the light-regulation and circadian-rhythm of the VDE gene promoter from Zingiber officinale

Zhao

Wang

et al. 2012

Plant Cell Rep

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Ginger (Zingiber officinale Rosc.) is prone to photoinhibition under intense sunlight. Excessive light can be dissipated by the xanthophyll cycle, where violaxanthin de-epoxidase (VDE) plays a critical role in protecting the photosynthesis apparatus from the damage of excessive light. We isolated *2.0 kb of ginger VDE (GVDE) gene promoter, which contained the circadian box, I-box, G-box and GT-1 motif. Histochemical staining of Arabidopsis indicated the GVDE promoter was active in almost all organs, especially green tissues. b-glucuronidase (GUS) activity driven by GVDE promoter was repressed rather than activated by high light. GUS activity was altered by hormones, growth regulators and abiotic stresses, which increased with 2,4-dichlorophenoxyacetic acid and decreased with abscisic acid, salicylic acid, zeatin, salt (sodium chloride) and polyethylene glycol. Interestingly, GUS activities with gibberellin or indole-3-acetic acid increased in the short-term (24 h) and decreased in the long-term (48 and 72 h). Analysis of 5 0 flank deletion found two crucial functional regions residing in -679 to -833 and -63 to -210. Northern blotting analysis found transcription to be regulated by the endogenous circadian clock. Finally, we found a region necessary for regulating the circadian rhythm and another for the basic promoter activity. Key message A novel promoter, named GVDE promoter, was first isolated and analyzed in this study. We have determined one region crucial for promoter activity and another responsible for keeping circadian rhythms.

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Molecular cloning and characterization of the light-regulation and circadian-rhythm of the VDE gene promoter from Zingiber officinale

Zhao

Wang

et al. 2012

Plant Cell Rep

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“…Thus, much more excessive energy is converted into excited energy and reactive oxygen species (ROS) [1] causing inhibition of the repair mechanism for photosystem II (PSII) [2]. Thus, ROS are an extremely harmful stress factor for photosynthetic organisms [3]. During chilling stress, ROS are mainly formed in chloroplasts, resulting in cellular oxidative damage [4] and inhibiting the repair of PSII by suppressing D1 protein synthesis, which acts as the primary target for photodamage of the PSII complex when plants suffer from photoinhibition [5,6].…”

Section: Introductionmentioning

confidence: 99%

“…It has further been shown in many studies that Z is an intermediate in the biosynthetic pathway of abscisic acid (ABA), an important plant hormone associated with stress response and dormancy regulation [12]. Full-length cDNA of the VDE gene has been cloned in Arabidopsis, spinach, wheat, tomato and other plants [3,13]. But the ZE genes have only been cloned in a few plants [14,15] and there are few reports on the purification of the translated proteins.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and characterization of ClZE, a zeaxanthin epoxidase gene in watermelon (Citrullus lanatus)

Liu

Yao

et al. 2017

Biotechnology & Biotechnological Equipment

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In this study, a watermelon (Citrullus lanatus) zeaxanthin epoxidase gene, ClZE, was isolated by reverse transcription-polymerase chain reaction (PCR) together with RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE). The full cDNA sequence of ClZE is 2535 bp in length containing a 1998 bp open reading frame (ORF) that encodes 665 amino acids. ClZE was shown to share high homology with the putative ZE genes in other plant species. Prediction analysis revealed that ClZE bears two large conservative domains, including Pyr_redox and FHA (forkheadassociated domain). Phylogenetic analysis suggested that ClZE shares high similarity (94.8%) with CsZE from Cucumis sativus, but is far from the ZE genes of other species. Prokaryotic expression indicated that ClZE possessed the apparent molecular mass consistent with its calculated molecular mass of 73.1 KDa. Based on quantitative real-time PCR (qRT-PCR), ClZE was shown to be down-regulated under chilling-low irradiance stress in watermelon leaves. Transgenic Arabidopsis lines harbouring ClZE were generated to test the gene function in mediating plant response to chilling stress. The results indicated that the transgenic lines exhibited decreased nonphotochemical quenching (NPQ) and maximum quantum efficiency of photosystem II (PSII) photochemistry (Fv/Fm), increased activities of peroxidase (POD), superoxide dismutase (SOD) and elevated content of malondialdehyde (MDA) relative to the wild type (WT) under chilling-low irradiance stress. In addition, overexpression of ClZE resulted in impaired xanthophyll cycle and aggravated PSII photoinhibition under chilling-low irradiance. All the results together suggested that ClZE plays major roles in regulating the photoinhibition behaviour.

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Tomato (Solanum lycopersicum)

León‐García

Coronel²,

Vela‐Gutiérrez³

et al. 2017

Fruit and Vegetable Phytochemicals

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Overexpression of violaxanthin de-epoxidase gene alleviates photoinhibition of PSII and PSI in tomato during high light and chilling stress

Cited by 63 publications

References 44 publications

Molecular cloning and characterization of the light-regulation and circadian-rhythm of the VDE gene promoter from Zingiber officinale

Molecular cloning and characterization of the light-regulation and circadian-rhythm of the VDE gene promoter from Zingiber officinale

Molecular cloning and characterization of ClZE, a zeaxanthin epoxidase gene in watermelon (Citrullus lanatus)

Tomato (Solanum lycopersicum)

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