Overexpression of WUSCHEL in C. chinense causes ectopic morphogenesis

Solís-Ramos, Laura Y.; González-Estrada, Tomas; Nahuath-Dzib, Sara; Zapata-Rodriguez, L. C.; Castaño, Enrique

doi:10.1007/s11240-008-9485-7

Cited by 45 publications

(40 citation statements)

References 29 publications

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“…C. chinense is no exception (Santana-Buzzy et al 2005;López-Puc et al 2006), and no efficient, reproducible regeneration system has yet been developed for this species. A dependable regeneration system is indispensable for genetic improvement, and is also important to conduct studies to establish a reliable genetic transformation protocol, as it can be an alternative to abate recalcitrance of tissues to in vitro regeneration, besides being a useful tool for genetic improvement (Zuo et al 2002;Solís-Ramos et al 2009). …”

mentioning

confidence: 99%

Endogenous GUS-like activity in Capsicum chinense Jacq.

Solís-Ramos¹,

González-Estrada²,

Andrade‐Torres³

et al. 2010

Electron. J. Biotechnol.

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mentioning

confidence: 99%

Endogenous GUS-like activity in Capsicum chinense Jacq.

Solís-Ramos¹,

González-Estrada²,

Andrade‐Torres³

et al. 2010

Electron. J. Biotechnol.

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“…In this construct the gene WUSCHEL is under an estradiol inducible promoter (Zuo et al 2002). Therefore we would expect that treatment with estradiol will lead to over-expression of WUSCHEL in transformed coconut embryogenic calli, promoting somatic embryo formation, as reported for Capsicum chinense cultures (Solís-Ramos et al 2009). This test will be carried out as a follow up of the present study, as an approach to increase the efficiency of somatic embryo formation in embryogenic calli in order to improve the micropropagation of coconut (Pérez-Núñez et al 2006).…”

Section: Discussionmentioning

confidence: 88%

“…For experiments on the combined protocol for transformation, the C58C1 strain of A. tumefaciens with the pER10W-35SRed plasmid or the disarmed C58C1 strain was used. The pER10W-35SRed plasmid contains in T-DNA the DsRFP reporter gene under the constitutive 35S promoter, the selection gene nptII (neomycin phosphotransferase II), also under the control of 35S promoter, and the embryogenesis related gene WUSCHEL from A. thaliana (Zuo et al 2002;Canche-Moo et al 2006;Solís-Ramos et al 2009). …”

Section: Strains and Plasmidsmentioning

confidence: 99%

“…The minimum effective kanamycin concentration 300 mg/L was still higher in comparison with concentrations reported for other species. For example, the transformation protocol for Agave salmiana includes 50 mg/L kanamycin (FloresBenítez et al 2007), 100 mg/L for Coffea canephora and Capsicum chinense (Canche-Moo et al 2006;Solís-Ramos et al 2009), and 150 mg/L for Nicotiana tobaccum and Nicotiana benthamiana (Clemente 2006). Coconut is thus apparently more tolerant to the effect of kanamycin than the species mentioned above.…”

Section: Kanamycin For Transformed Plant Cells Selectionmentioning

confidence: 99%

“…This has been achieved by insertion and over-expression of genes related to the control of morphogenesis, such as the heterologous gene WUSCHEL in Arabidopsis thaliana and Coffea canephora cultures that promoted the transition from vegetative to embryogenic state, and eventually led to somatic embryo formation (Zuo et al 2002;Arroyo-Herrera et al 2008). In Capsicum chinense, the induced expression of WUSCHEL in segments of transformed stems began to form globular structures, suggesting that heterologous WUSCHEL was active and involved in the process of morphogenesis (Solís-Ramos et al 2009, 2010b. It has been demonstrated in Arabidopsis that over-expression of a somatic embryogenesis receptor-like kinase (SERK) gene AtSERK1 increases the embryogenic competence of callus derived from transformed seedlings 3 to 4-fold when compared with the wild-type callus (Hecht et al 2001).…”

Section: Introductionmentioning

confidence: 99%

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Transient genetic transformation of embryogenic callus of Cocos nucifera

et al. 2011

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Coconut palm (Cocos nucifera) is a plant species recalcitrant to in vitro morphogenesis and no protocols for the genetic transformation of coconut tissues have been published. The present study aimed to develop a protocol for genetic transformation of this palm species; evaluating reporter genes, transformation methods, and conditions for the use of antibiotics to select transformed plant cells. The gene gusA was first used for Agrobacterium tumefaciens mediated transformation of coconut embryogenic calli. However, endogenous GUS-like activity was found in calli not co-cultured with bacteria. Then essays for Agrobacterium-mediated transformation were developed using green and red fluorescent genes. Both genes are suitable as reporter genes for coconut transformation. In order to establish a protocol for coconut genetic transformation, an approach was used that combined biobalistics to generate micro-wounds in explants, vacuum infiltration and co-culture with Agrobacterium tumefaciens (C58C1 + pER10W-35SRed containing the embryogenesis related gene WUSCHEL). Calli treated with the combined protocol showed red fluorescence with greater intensity and greater area than calli treated with either biobalistics or infiltration, followed by bacteria co-culture. PCR amplification of DNA extracts from transformed embryogenic callus produced a band with the expected size using WUSCHEL primers (862 bp). No band was obtained using the VirE2 primers. This is the first report of transient genetic transformation of C. nucifera and it is the first step toward a protocol that will be useful for the study of the role of genes of interest and for practical applications, such as the improvement of coconut micropropagation via somatic embryogenesis.

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Transcription Factors in the Regulation of Somatic Embryogenesis

Nowak

Gaj

2016

Somatic Embryogenesis: Fundamental Aspects and Applications

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Somatic embryogenesis (SE), the process through which already differentiated cells reverse their developmental programme and become embryogenic, requires drastic changes in the transcriptome of the explant cells. Among the various factors that underlie this developmental switch, genes encoding transcription factors (TFs), which constitute the sequence-specific DNA-binding proteins, are widely accepted as playing a central function in the gene expression regulation. In recent years, intensive analysis of the global transcriptomes of plant cells that are undergoing embryogenic transition and the use of Arabidopsis (a model in plant genomics) in studies on the genetic control of SE have substantially contributed to the identification of SE regulators. A survey of SE-associated transcriptomes illustrated the combinational effects of stress and hormone signalling that are related to the in vitro environment that is imposed during a culture. Accordingly, among the TFs that are considered to be essential in SE induction, those that are involved in stress and hormone plant responses and especially flower development were found to be most frequent. This chapter provides a comprehensive review of the current knowledge about the TFs that are involved in the induction of SE in plant explants that are cultured in vitro. In addition to a general characterisation of the TF transcriptomes that are associated with SE induction in different plants, the individual TF genes with documented functions in the regulation of SE are presented with a special reference to their possible targets and the TF-controlled molecular mechanisms that underlie SE induction.

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Overexpression of WUSCHEL in C. chinense causes ectopic morphogenesis

Cited by 45 publications

References 29 publications

Endogenous GUS-like activity in Capsicum chinense Jacq.

Endogenous GUS-like activity in Capsicum chinense Jacq.

Transient genetic transformation of embryogenic callus of Cocos nucifera

Transcription Factors in the Regulation of Somatic Embryogenesis

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