Overexpression of YAP2, coding for a new yAP protein, and YAP1 in Saccharomyces cerevisiae alleviates growth inhibition caused by 1,10-phenanthroline.

Bossier, Peter; Fernandes, Lisete; Rocha, Da; Rodrigues-Pousada, Claudina

doi:10.1016/s0021-9258(19)49510-0

Cited by 80 publications

(14 citation statements)

References 35 publications

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“…The YAP2 gene was identified by its ability, when in high copy number, to confer resistance to cadmium and by the hypersensitivity of yap2 null mutants to oxidants. 7,58,151 The role of the YAP2 gene in oxidant-dependent gene regulation is still unclear, as no target genes for Yap2p have yet been identified. However, Yap2p clearly plays a role in regulating the H 2 O 2 -adaptive stress response, since induction of this adaptive stress response was diminished in a yap2 null mutant.…”

Section: Bzip Transcription Factorsmentioning

confidence: 99%

Oxidative stress responses of the yeastSaccharomyces cerevisiae

Jamieson

1998

Yeast

663

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All aerobically growing organisms suffer exposure to oxidative stress, caused by partially reduced forms of molecular oxygen, known as reactive oxygen species (ROS). These are highly reactive and capable of damaging cellular constituents such as DNA, lipids and proteins. Consequently, cells from many different organisms have evolved mechanisms to protect their components against ROS. This review concentrates on the oxidant defence systems of the budding yeast Saccharomyces cerevisiae , which appears to have a number of inducible adaptive stress responses to oxidants, such as H2 O2 , superoxide anion and lipid peroxidation products. The oxidative stress responses appear to be regulated, at least in part, at the level of transcription and there is considerable overlap between them and many diverse stress responses, allowing the yeast cell to integrate its response towards environmental stress. © 1998 John Wiley & Sons, Ltd.

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Section: Bzip Transcription Factorsmentioning

confidence: 99%

Oxidative stress responses of the yeastSaccharomyces cerevisiae

Jamieson

1998

Yeast

663

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“…A second AP‐1‐like gene, yAP‐2, has also been isolated from S.cerevisiae (Bossier et al ., 1993; Wu et al ., 1993; Hirata et al ., 1994). Although it has a similar DNA‐binding specificity to yAP‐1 (Wu et al ., 1993), it seems not to play a major function in the oxidative stress response since its deletion does not strongly affect the sensitivity to such stress (Hirata et al ., 1994).…”

Section: Introductionmentioning

confidence: 99%

Regulation of yAP-1 nuclear localization in response to oxidative stress

1997

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The YAP1 gene of Saccharomyces cerevisiae encodes a bZIP‐containing transcription factor that is essential for the normal response of cells to oxidative stress. Under stress conditions, the activity of yAP‐1 is increased, leading to the induced expression of a number of target genes encoding protective enzymes or molecules. We have examined the mechanism of this activation. Upon imposition of oxidative stress, a small increase in the DNA‐binding capacity of yAP‐1 occurs. However, the major change is at the level of nuclear localization; upon induction the yAP‐1 protein relocalizes from the cytoplasm to the nucleus. This regulated localization is mediated by a cysteine‐rich domain (CRD) at the C‐terminus, its removal resulting in constitutive nuclear localization and high level activity. Furthermore, the CRD of yAP‐1 is sufficient to impose regulated nuclear localization of the GAL4 DNA‐binding domain. Amino acid substitutions indicated that three conserved cysteine residues in the CRD are essential for the regulation. We suggest therefore, that these cysteine residues are important in sensing the redox state of the cell and hence regulating yAP‐1 activity.

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“…Conventional sequencing of selected genes originally identified a number of S.cerevisiae mRNAs that contain uORFs (4,(15)(16)(17)(18)(19) and the more recent results of the yeast genome sequencing project have increased the number of known cases considerably. Moreover, a large percentage of these genes encode regulatory proteins (6,20), suggesting a possible common mechanism of post-transcriptional control of these genes' expression, mediated by the upstream regulatory sequences.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, a large percentage of these genes encode regulatory proteins (6,20), suggesting a possible common mechanism of post-transcriptional control of these genes' expression, mediated by the upstream regulatory sequences. Two striking examples of uORF-containing mRNAs encode Yap1p and Yap2p (15,17,21,22), both of which manifest strong homology to AP1-like factors in complex eukaryotes and to Gcn4p in yeast. Like the GCN4 gene, YAP1 and YAP2 are also regulatory genes involved in the mechanisms used by the yeast cell to protect itself in situations of (severe) stress.…”

Section: Introductionmentioning

confidence: 99%

“…Like the GCN4 gene, YAP1 and YAP2 are also regulatory genes involved in the mechanisms used by the yeast cell to protect itself in situations of (severe) stress. Overexpression of the two related YAP1 and YAP2 genes confers general stress resistance to a variety of unrelated compounds, from metal ions to different inhibitors and drugs (15,(22)(23)(24)(25)(26)(27)(28). Loss of function mutations in YAP1 result in hypersensitivity to hydrogen peroxide, suggesting a role in the cellular response to oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

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The yeast transcription factor genes YAP1 and YAP2 are subject to differential control at the levels of both translation and mRNA stability

Vilela

Linz

Rodrigues-Pousada

et al. 1998

Nucleic Acids Research

100

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Two forms of post-transcriptional control direct differential expression of the Saccharomyces cerevisiae genes encoding the AP1-like transcription factors Yap1p and Yap2p. The mRNAs of these genes contain respectively one (YAP1 uORF) and two (YAP2 uORF1 and uORF2) upstream open reading frames. uORF-mediated modulation of post-termination events on the 5'-untranslated region (5'-UTR) directs differential control not only of translation but also of mRNA decay. Translational control is defined by two types of uORF function. The YAP1 -type uORF allows scanning 40S subunits to proceed via leaky scanning and re-initiation to the major ORF, whereas the YAP2 -type acts to block ribosomal scanning by promoting efficient termination. At the same time, the YAP2 uORFs define a new type of mRNA destabilizing element. Both post-termination ribosome scanning behaviour and mRNA decay are influenced by the coding sequence and mRNA context of the respective uORFs, including downstream elements. Our data indicate that release of post-termination ribosomes promotes largely upf -independent accelerated decay. It follows that translational termination on the 5'-UTR of a mature, non-aberrant yeast mRNA can trigger destabilization via a different pathway to that used to rid the cell of mRNAs containing premature stop codons. This route of control of non-aberrant mRNA decay influences the stress response in yeast. It is also potentially relevant to expression of the sizable number of eukaryotic mRNAs that are now recognized to contain uORFs.

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Overexpression of YAP2, coding for a new yAP protein, and YAP1 in Saccharomyces cerevisiae alleviates growth inhibition caused by 1,10-phenanthroline.

Cited by 80 publications

References 35 publications

Oxidative stress responses of the yeastSaccharomyces cerevisiae

Oxidative stress responses of the yeastSaccharomyces cerevisiae

Regulation of yAP-1 nuclear localization in response to oxidative stress

The yeast transcription factor genes YAP1 and YAP2 are subject to differential control at the levels of both translation and mRNA stability

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