Overexpression of α-Tubulin Gene of Sugarcane (Saccharum spp. hybrids), SoTUA, Enhances Tobacco Tolerance to Cold Stress

Chen, Jiao-Yun; Sun, Bo; Khan, Qaisar; LiTao, Yang; Zhang, Baoqing; Xing, Yong‐Xiu; Li, Yang–Rui

doi:10.1007/s12355-022-01118-2

Cited by 2 publications

(1 citation statement)

References 45 publications

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“…Three TVD sugarcane leaves were collected from each treatment, and sampling was conducted at 0, 12, 24, 48, and 72 hpi. The activity of peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) was determined according to the method of Deng et al (2020), malondialdehyde (MDA) content was determined using the spectrophotometric thiobarbituric acid reactive substances (TBARS) method as described by Chen et al (2022). Hydrogen peroxide (H 2 O 2 ) was measured using the iodometric method (Junglee et al, 2014).…”

Section: Assays Of Defence-related Physiological Parameters In Sugarc...mentioning

confidence: 99%

Silicon induces ROS scavengers, hormone signalling, antifungal metabolites, and silicon deposition against brown stripe disease in sugarcane

Chen,

Li,

Zeng

et al. 2024

Physiologia Plantarum

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Bipolaris setariae is known to cause brown stripe disease in sugarcane, resulting in significant yield losses. Silicon (Si) has the potential to enhance plant growth and biotic resistance. In this study, the impact of Si on brown stripe disease was investigated across susceptible and resistant sugarcane varieties, utilizing four Si concentrations (0, 15, 30, and 45 g per barrel of Na2SiO3·5H2O). Si significantly reduced the incidence of brown stripe disease (7.41–59.23%) and alleviated damage to sugarcane growth parameters, photosynthetic parameters, and photosynthetic pigments. Submicroscopic observations revealed that Si induced the accumulation of silicified cells in leaves, reduced spore accumulation, decreased stomatal size, and protected organelles from B. setariae damage. In addition, Si increased the activity of antioxidant enzymes (superoxide dismutase, peroxidase, and catalase), reduced reactive oxygen species production (malondialdehyde and hydrogen peroxide) and modulated the expression of genes associated with hormone signalling (PR1, TGA, AOS, AOC, LOX, PYL8, and SnRK2), leading to the accumulation of abscisic acid and jasmonic acid and inhibiting SA synthesis. Si also activated the activity of metabolism‐related enzymes (polyphenol oxidase and phenylalanine ammonia lyase) and the gene expression of PAL‐dependent genes (PAL, C4H, and 4CL), regulating the accumulation of metabolites, such as chlorogenic acid and lignin. The antifungal test showed that chlorogenic acid (15ug μL−1) had a significant inhibitory effect on the growth of B. setariae. This study is the first to demonstrate the inhibitory effect of Si on B. setariae in sugarcane, highlighting Si as a promising and environmentally friendly strategy for managing brown stripe disease.

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Section: Assays Of Defence-related Physiological Parameters In Sugarc...mentioning

confidence: 99%

Silicon induces ROS scavengers, hormone signalling, antifungal metabolites, and silicon deposition against brown stripe disease in sugarcane

Chen,

Li,

Zeng

et al. 2024

Physiologia Plantarum

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Genome-wide identification and evolution of the tubulin gene family in Camelina sativa

Blume,

Rabokon,

Pydiura

et al. 2024

BMC Genomics

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Background Tubulins play crucial roles in numerous fundamental processes of plant development. In flowering plants, tubulins are grouped into α-, β- and γ-subfamilies, while α- and β-tubulins possess a large isotype diversity and gene number variations among different species. This circumstance leads to insufficient recognition of orthologous isotypes and significantly complicates extrapolation of obtained experimental results, and brings difficulties for the identification of particular tubulin isotype function. The aim of this research is to identify and characterize tubulins of an emerging biofuel crop Camelina sativa. Results We report comprehensive identification and characterization of tubulin gene family in C. sativa, including analyses of exon-intron organization, duplicated genes comparison, proper isotype designation, phylogenetic analysis, and expression patterns in different tissues. 17 α-, 34 β- and 6 γ-tubulin genes were identified and assigned to a particular isotype. Recognition of orthologous tubulin isotypes was cross-referred, involving data of phylogeny, synteny analyses and genes allocation on reconstructed genomic blocks of Ancestral Crucifer Karyotype. An investigation of expression patterns of tubulin homeologs revealed the predominant role of N6 (A) and N7 (B) subgenomes in tubulin expression at various developmental stages, contrarily to general the dominance of transcripts of H7 (C) subgenome. Conclusions For the first time a complete set of tubulin gene family members was identified and characterized for allohexaploid C. sativa species. The study demonstrates the comprehensive approach of precise inferring gene orthology. The applied technique allowed not only identifying C. sativa tubulin orthologs in model Arabidopsis species and tracking tubulin gene evolution, but also uncovered that A. thaliana is missing orthologs for several particular isotypes of α- and β-tubulins.

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Overexpression of α-Tubulin Gene of Sugarcane (Saccharum spp. hybrids), SoTUA, Enhances Tobacco Tolerance to Cold Stress

Cited by 2 publications

References 45 publications

Silicon induces ROS scavengers, hormone signalling, antifungal metabolites, and silicon deposition against brown stripe disease in sugarcane

Silicon induces ROS scavengers, hormone signalling, antifungal metabolites, and silicon deposition against brown stripe disease in sugarcane

Genome-wide identification and evolution of the tubulin gene family in Camelina sativa

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