Overexpression ofapc10 + in fission yeast can suppress the temperature sensitivity ofnuc2-663 mutant but not its sterility

Grishchuk, Ekaterina L.; Frolov, D. Yu.; Savchenko, G. V.

doi:10.1007/bf02759608

Cited by 2 publications

(1 citation statement)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PCR was used to amplify a continuous ∼3.5 kb fragment containing the Pk1 and GFP tags together with the ura4 + gene. For PCR, we followed published protocols (Grishchuk et al ., 2000; Innis et al ., 1990). The GFP-Pk1- ura4 + PCR product was used directly to transform cells (SP 26), and Ura + colonies were selected for further analysis.…”

Section: Methodsmentioning

confidence: 99%

Tubulin heterodimers remain functional for one cell cycle after the inactivation of tubulin‐folding cofactor D in fission yeast cells

Fedyanina

Book

Grishchuk

2009

Yeast

View full text Add to dashboard Cite

Tubulin-folding cofactor D plays a major role in the formation of functional tubulin heterodimers, the subunits of microtubules (MTs) that are essential for cell division. Previous work has suggested that, in Schizosaccharomyces pombe, cofactor D function is required during G1 or S phases of the cell cycle, and when it fails to function due to the temperature-sensitive mutation alp1-t1, cells are unable to segregate their chromosomes in the subsequent mitosis. Here we report that another mutation in the cofactor D gene, alp1-1315, causes failures in either the first or second mitosis in cells synchronized in G1 or G2 phases, respectively. Other results, however, suggest that the kinetics of viability loss in these mutants does not depend on progression through the cell cycle. When cofactor D function is perturbed in cells blocked in G2, cytoplasmic MTs appear normal for 2–3 h but thereafter they disintegrate quickly, so that only a few short MTs remain. These residual MTs are, however, stably maintained, suggesting that they do not require active cofactor D function. The abrupt disassembly of MT cytoskeleton at restrictive temperature in non-cycling cofactor D mutant cells strongly suggests that the life-span of folded tubulin dimers might be downregulated. Indeed, this period is significantly shorter than the previously determined dissociation time of bovine tubulins in vitro. The death of mutant cells occurs inevitably after 2–3 h at restrictive temperature in the following mitosis, and is explained by the idea that MT structures formed in the absence of cofactor D cannot support normal cell division.

show abstract

Section: Methodsmentioning

confidence: 99%

Tubulin heterodimers remain functional for one cell cycle after the inactivation of tubulin‐folding cofactor D in fission yeast cells

Fedyanina

Book

Grishchuk

2009

Yeast

View full text Add to dashboard Cite

show abstract

Current Awareness

2001

Yeast

View full text Add to dashboard Cite

In order to keep subscribers up‐to‐date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly‐published material on yeasts. Each bibliography is divided into 10 sections. 1 Books, Reviews & Symposia; 2 General; 3 Biochemistry; 4 Biotechnology; 5 Cell Biology; 6 Gene Expression; 7 Genetics; 8 Physiology; 9 Medical Mycology; 10 Recombinant DNA Technology. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted. (5 weeks journals ‐ search completed 6th Dec. 2000)

show abstract

Overexpression ofapc10 + in fission yeast can suppress the temperature sensitivity ofnuc2-663 mutant but not its sterility

Cited by 2 publications

References 23 publications

Tubulin heterodimers remain functional for one cell cycle after the inactivation of tubulin‐folding cofactor D in fission yeast cells

Tubulin heterodimers remain functional for one cell cycle after the inactivation of tubulin‐folding cofactor D in fission yeast cells

Current Awareness

Contact Info

Product

Resources

About