2021
DOI: 10.3390/v13081604
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Overlap Intensity: An ImageJ Macro for Analyzing the HIV-1 In Situ Uncoating Assay

Abstract: Capsid uncoating is at the crossroads of early steps in HIV-1 replication. In recent years, the development of novel assays has expanded how HIV-1 uncoating can be studied. In the in situ uncoating assay, dual fluorescently labelled virus allows for the identification of fused viral cores. Antibody staining then detects the amount of capsid associated with each viral core at different times post-infection. Following fixed cell imaging, manual counting can be used to assess the fusion state and capsid signal fo… Show more

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Cited by 1 publication
(2 citation statements)
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“…Images were captured by a fluorescence microscope (Nikon, Japan). Fluorescence intensity was quantified by ImageJ as previously described . The primary antibodies were used as follows: anti-NLRP3 (Cat.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Images were captured by a fluorescence microscope (Nikon, Japan). Fluorescence intensity was quantified by ImageJ as previously described . The primary antibodies were used as follows: anti-NLRP3 (Cat.…”
Section: Methodsmentioning
confidence: 99%
“…Fluorescence intensity was quantified by ImageJ as previously described. 23 The primary antibodies were used as follows: anti-NLRP3 (Cat. AF2155, Beyotime, Shanghai, China), anti-ASC (Cat.…”
Section: Immunofluorescencementioning
confidence: 99%