Overlapping responses to multiple abiotic stresses in citrus: from mechanism understanding to genetic improvement

Dahro, Bachar; Li, Chunlong; Liu, Jihong

doi:10.1007/s44281-023-00007-2

Cited by 15 publications

(4 citation statements)

References 157 publications

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“…Water is an important factor for citrus crops. Limited water availability occurs in most citrus-producing countries and restricts citrus production (Dahro et al, 2023). Generally, citrus crops require 840-1220 mm of water annually (Enciso et al, 2005), in which plants during the fruit expansion period require more water while requirements decrease during the fruit maturity period.…”

Section: Introductionmentioning

confidence: 99%

Seasonal drought promotes citrate accumulation in citrus fruit through the CsABF3‐activated CsAN1‐CsPH8 pathway

Ma,

Sheng,

et al. 2024

New Phytologist

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Summary Plenty of rainfall but unevenly seasonal distribution happens regularly in southern China. Seasonal drought from summer to early autumn leads to citrus fruit acidification, but how seasonal drought regulates citrate accumulation remains unknown. Herein, we employed a set of physiological, biochemical, and molecular approaches to reveal that CsABF3 responds to seasonal drought stress and modulates citrate accumulation in citrus fruits by directly regulating CsAN1 and CsPH8. Here, we demonstrated that irreversible acidification of citrus fruits is caused by drought lasting for > 30 d during the fruit enlargement stage. We investigated the transcriptome characteristics of fruits affected by drought and corroborated the pivotal roles of a bHLH transcription factor (CsAN1) and a P3A‐ATPase gene (CsPH8) in regulating citrate accumulation in response to drought. Abscisic acid (ABA)‐responsive element binding factor 3 (CsABF3) was upregulated by drought in an ABA‐dependent manner. CsABF3 activated CsAN1 and CsPH8 expression by directly and specifically binding to the ABA‐responsive elements (ABREs) in the promoters and positively regulated citrate accumulation. Taken together, this study sheds new light on the regulatory module ABA‐CsABF3‐CsAN1‐CsPH8 responsible for citrate accumulation under drought stress, which advances our understanding of quality formation of citrus fruit.

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Section: Introductionmentioning

confidence: 99%

Seasonal drought promotes citrate accumulation in citrus fruit through the CsABF3‐activated CsAN1‐CsPH8 pathway

Ma,

Sheng,

et al. 2024

New Phytologist

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“…Drought is the main abiotic factor affecting plant development and yield. In general, plants can actively maintain water balance by increasing the ability of roots to draw water from the soil, by closing stomata to minimize water loss, and by regulating permeability within tissues (Dahro et al, 2023;Rodrigues et al, 2019). It is well-established that water deficiency disrupts source-sink relationships of sugars and that plants increase root sugar content, which may allow them to better survive under water deficit conditions (Hennion et al, 2019;Lemoine et al, 2013;Reinders et al, 2006) as sugars can provide osmotic adjustment, thus protecting cellular structures (Yokota et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Glucose uptake from the rhizosphere mediated by MdDOF3‐MdHT1.2 regulates drought resistance in apple

Tian,

Li,

Wang

et al. 2024

Plant Biotechnology Journal

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SummaryIn plants under drought stress, sugar content in roots increases, which is important for drought resistance. However, the molecular mechanisms for controlling the sugar content in roots during response to drought remain elusive. Here, we found that the MdDOF3‐MdHT1.2 module‐mediated glucose influx into the root is essential for drought resistance in apple (Malus × domestica). Drought induced glucose uptake from the rhizosphere and up‐regulated the transcription of hexose transporter MdHT1.2. Compared with the wild‐type plants, overexpression of MdHT1.2 promoted glucose uptake from the rhizosphere, thereby facilitating sugar accumulation in root and enhancing drought resistance, whereas silenced plants showed the opposite phenotype. Furthermore, ATAC‐seq, RNA‐seq and biochemical analysis demonstrated that MdDOF3 directly bound to the promoter of MdHT1.2 and was strongly up‐regulated under drought. Overexpression of MdDOF3 in roots improved MdHT1.2‐mediated glucose transport capacity and enhanced plant resistance to drought, but MdDOF3‐RNAihr apple plants showed the opposite phenotype. Moreover, overexpression of MdDOF3 in roots did not attenuate drought sensitivity in MdHT1.2‐RNAi plants, which was correlated with a lower glucose uptake capacity and glucose content in root. Collectively, our findings deciphered the molecular mechanism through which glucose uptake from the rhizosphere is mediated by MdDOF3‐MdHT1.2, which acts to modulate sugar content in root and promote drought resistance.

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“…Citrus is one of the most valuable fruits worldwide and has been cultivated in China for more than 4000 years (Wu et al, 2014;Wu et al, 2018). At present, the citrus industry has become a mainstay for rural economy of the main production areas in the south of China, which has contributed positively to promoting the income of farmers, expanding the employment of urban and rural residents, and improving the ecological environment (Dahro et al, 2023). Since most citrus varieties are polyembryonic and asexual embryos develop superiorly to sexual embryos, the efficiency of citrus breeding through sexual crosses is relatively low (Zhang et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Highly efficient Agrobacterium rhizogenes-mediated hairy root transformation in citrus seeds and its application in gene functional analysis

Wang,

Qin,

Wei

et al. 2023

Front. Plant Sci.

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Highly efficient genetic transformation technology is beneficial for plant gene functional research and molecular improvement breeding. However, the most commonly used Agrobacterium tumefaciens-mediated genetic transformation technology is time-consuming and recalcitrant for some woody plants such as citrus, hampering the high-throughput functional analysis of citrus genes. Thus, we dedicated to develop a rapid, simple, and highly efficient hairy root transformation system induced by Agrobacterium rhizogenes to analyze citrus gene function. In this report, a rapid, universal, and highly efficient hairy root transformation system in citrus seeds was described. Only 15 days were required for the entire workflow and the system was applicable for various citrus genotypes, with a maximum transformation frequency of 96.1%. After optimization, the transformation frequency of Citrus sinensis, which shows the lowest transformation frequency of 52.3% among four citrus genotypes initially, was increased to 71.4% successfully. To test the applicability of the hairy roots transformation system for gene functional analysis of citrus genes, we evaluated the subcellular localization, gene overexpression and gene editing in transformed hairy roots. Compared with the traditional transient transformation system performed in tobacco leaves, the transgenic citrus hairy roots displayed a more clear and specific subcellular fluorescence localization. Transcript levels of genes were significantly increased in overexpressing transgenic citrus hairy roots as compared with wild-type (WT). Additionally, hairy root transformation system in citrus seeds was successful in obtaining transformants with knocked out targets, indicating that the Agrobacterium rhizogenes-mediated transformation enables the CRISPR/Cas9-mediated gene editing. In summary, we established a highly efficient genetic transformation technology with non-tissue-culture in citrus that can be used for functional analysis such as protein subcellular localization, gene overexpression and gene editing. Since the material used for genetic transformation are roots protruding out of citrus seeds, the process of planting seedlings prior to transformation of conventional tissue culture or non-tissue-culture was eliminated, and the experimental time was greatly reduced. We anticipate that this genetic transformation technology will be a valuable tool for routine research of citrus genes in the future.

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Overlapping responses to multiple abiotic stresses in citrus: from mechanism understanding to genetic improvement

Cited by 15 publications

References 157 publications

Seasonal drought promotes citrate accumulation in citrus fruit through the CsABF3‐activated CsAN1‐CsPH8 pathway

Seasonal drought promotes citrate accumulation in citrus fruit through the CsABF3‐activated CsAN1‐CsPH8 pathway

Glucose uptake from the rhizosphere mediated by MdDOF3‐MdHT1.2 regulates drought resistance in apple

Highly efficient Agrobacterium rhizogenes-mediated hairy root transformation in citrus seeds and its application in gene functional analysis

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