Overnight incubation improves selection of frozen–thawed blastocysts for transfer: preliminary study using supernumerary embryos

Guérif, Fabrice; Cadoret, Véronique; Poindron, J.; Lansac, J.; Royère, Dominique

doi:10.1016/s0093-691x(03)00130-4

Cited by 26 publications

(26 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This re-expansion rate is higher than previously reported for in vitro produced embryos, but is similar to in vivo derived, vitrified ovine embryos (Dattena et al 2000). As observed in humans (Guerif et al 2003), the pregnancy rate was similar for embryos transferred into ewes within a few hours of thawing and embryos that were thawed and cultured overnight prior to transfer.…”

Section: Discussionsupporting

confidence: 77%

Birth of lambs of a pre-determined sex after in vitro production of embryos using frozen–thawed sex-sorted and re-frozen–thawed ram spermatozoa

Hollinshead

Evans

et al. 2004

Reproduction

View full text Add to dashboard Cite

The characteristics and functional capacity of ram spermatozoa frozen-thawed prior to and after flow cytometric sorting was assessed after incubation (37 8C; 6 h), in vitro fertilisation (IVF), and transfer of fresh and vitrified in vitro produced embryos. Frozen-thawed spermatozoa from two rams were allocated to four treatment groups: (i) non-sorted (Control); (ii) sorted (FS); (iii) sorted then re-frozen (FSF) and (iv) re-frozen control (FCF). Frozen-thawed samples were separated into X-and Y-chromosome bearing spermatozoa using a high-speed sperm sorter after density gradient centrifugation (X: 88 6 1.5% and Y: 87 6 1.1% purity). After 6 h incubation (37 8C), the percentage of motile spermatozoa was higher (P < 0.001) for FS (84 6 2.0%) compared with all other treatments (Control: 36 6 3.3%, FSF: 28 6 3.1%, FCF: 20 6 2.0%). In a sperm migration test greater numbers of FS spermatozoa penetrated 5 mm into the artificial cervical mucus compared with spermatozoa from all other treatments (152 6 39.4 vs 31 6 9.2 spermatozoa respectively; P < 0.05). Fertilisation and cleavage rates were higher (P < 0.05) for in vitro matured oocytes inseminated with Control compared with FSF spermatozoa. However, the Day 7 blastocyst development rate was higher for oocytes inseminated with FSF (62.2%) than FS and Control spermatozoa (52.7 and 50.0%; P < 0.05). The number of ewes pregnant (Day 60), lambing and the in vivo embryo survival rate was greater (P < 0.01) after the transfer of fresh embryos rather than vitrified embryos derived from X-and Y-spermatozoa (67.6, 64.7 and 41.2% vs 29.6, 25.9 and 14.8% respectively).Twenty-six of the 30 (86.7%) lambs derived from sex-sorted spermatozoa were of the correct sex. These results demonstrate that frozen-thawed ram spermatozoa can be sex-sorted for immediate or future use after re-cryopreservation and, in conjunction with IVF and embryo transfer, can be used to efficiently produce offspring of pre-determined sex.

show abstract

Section: Discussionsupporting

confidence: 77%

Birth of lambs of a pre-determined sex after in vitro production of embryos using frozen–thawed sex-sorted and re-frozen–thawed ram spermatozoa

Hollinshead

Evans

et al. 2004

Reproduction

View full text Add to dashboard Cite

show abstract

“…The fact that post-warm blastocysts with faster developmental kinetics resulted in higher implantation outcomes has been reported in humans [21,26,27] and sheep [28]. The criterion of fast-developing embryos employed in the present study (≥50% increase in diameter at 2-h culture) has been adopted from Shu et al [21].…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo viability of human blastocysts collapsed by laser pulse or osmotic shock prior to vitrification

Iwayama¹,

Hochi

Yamashita³

2010

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose This study was designed to investigate whether artificial shrinkage, induced by a laser pulse or hyperosmotic sucrose solutions, improves in vitro survival and/or implantation of vitrified-warmed human expanded blastocysts. Methods Before Cryotop vitrification, the blastocoelic cavity was collapsed either by a laser pulse or sucrose solutions. Non-treated blastocysts were used as control. Post-warm blastocyst survival and implantation after transfer were examined. Implantation rate outcome was retrospectively analyzed by morphological grading and developmental kinetics of post-warm blastocysts. Results Survival rates in the three groups were high. Implantation rates in the laser-pulse group (59.7%) were comparable with those in the sucrose group (49.3%), and were significantly higher than those in the control group (34.2%). The proportion of blastocysts showing fast development tended to be higher when the blastocysts underwent artificial shrinkage treatment before vitrification. There was no clear correlation between morphology of post-warm blastocysts and implantation rate. Conclusion Artificial shrinkage treatment before vitrification is associated with an increased probability of fast-developing embryos, resulting in higher implantation rates.

show abstract

“…Different from the large-sized blastomeres of cleavage-stage embryos (2-8-cell stage), the human blastocyst contains a large number (approximately 100) of two structurally and functionally differentiated cell populations: ICM and TE (9). These cells are small in size and volume; and blastocyst viability after post-thaw culture is less easy to define by detecting the further cleavage of survived blastomeres, even under inverted microscope (10).…”

mentioning

confidence: 99%

The value of fast blastocoele re-expansion in the selection of a viable thawed blastocyst for transfer

Shu

Watt

Gebhardt

et al. 2009

Fertility and Sterility

View full text Add to dashboard Cite

Overnight incubation improves selection of frozen–thawed blastocysts for transfer: preliminary study using supernumerary embryos

Cited by 26 publications

References 43 publications

Birth of lambs of a pre-determined sex after in vitro production of embryos using frozen–thawed sex-sorted and re-frozen–thawed ram spermatozoa

Birth of lambs of a pre-determined sex after in vitro production of embryos using frozen–thawed sex-sorted and re-frozen–thawed ram spermatozoa

In vitro and in vivo viability of human blastocysts collapsed by laser pulse or osmotic shock prior to vitrification

The value of fast blastocoele re-expansion in the selection of a viable thawed blastocyst for transfer

Contact Info

Product

Resources

About